Effect of methanogenic substrates on anaerobic oxidation of methane and sulfate reduction by an anaerobic methanotrophic enrichment

R.J.W. Meulepas, C.G. Jagersma, A.F. Khadem, C.J.N. Buisman, A.J.M. Stams, P.N.L. Lens

Research output: Contribution to journalArticleAcademicpeer-review

33 Citations (Scopus)

Abstract

Anaerobic oxidation of methane (AOM) coupled to sulfate reduction (SR) is assumed to be a syntrophic process, in which methanotrophic archaea produce an interspecies electron carrier (IEC), which is subsequently utilized by sulfate-reducing bacteria. In this paper, six methanogenic substrates are tested as candidate-IECs by assessing their effect on AOM and SR by an anaerobic methanotrophic enrichment. The presence of acetate, formate or hydrogen enhanced SR, but did not inhibit AOM, nor did these substrates trigger methanogenesis. Carbon monoxide also enhanced SR but slightly inhibited AOM. Methanol did not enhance SR nor did it inhibit AOM, and methanethiol inhibited both SR and AOM completely. Subsequently, it was calculated at which candidate-IEC concentrations no more Gibbs free energy can be conserved from their production from methane at the applied conditions. These concentrations were at least 1,000 times lower can the final candidate-IEC concentration in the bulk liquid. Therefore, the tested candidate-IECs could not have been produced from methane during the incubations. Hence, acetate, formate, methanol, carbon monoxide, and hydrogen can be excluded as sole IEC in AOM coupled to SR. Methanethiol did inhibit AOM and can therefore not be excluded as IEC by this study
Original languageEnglish
Pages (from-to)1499-1506
JournalApplied Microbiology and Biotechnology
Volume87
Issue number4
DOIs
Publication statusPublished - 2010

Keywords

  • anoxic marine sediment
  • microbial communities
  • electron-transfer
  • reducing bacteria
  • carbon-monoxide
  • archaea
  • energy
  • requirements
  • consumption
  • consortium

Fingerprint Dive into the research topics of 'Effect of methanogenic substrates on anaerobic oxidation of methane and sulfate reduction by an anaerobic methanotrophic enrichment'. Together they form a unique fingerprint.

Cite this