Projects per year
Abstract
Purpose
The purpose of this study was to determine the influence of Maillard reaction (MR, glycation) on biochemical and biological properties of the major peanut allergen Ara h 1.
Methods
Three different time/temperature conditions of treatment were applied (37, 60, and 145 °C). The extent of MR was assessed by SDS-PAGE, loss of free amino groups, fluorescence intensity, content of bound sugar and fructosamine. The Caco-2 model system was applied to study effects of hydrolysed and non-hydrolysed Ara h 1 on proliferation and interleukin-8 (IL-8) secretion from Caco-2 cells.
Results
We demonstrated significant differences in the biochemical properties of Ara h 1 glycated at different time/temperature conditions. Glycation of Ara h 1 at 37 °C was shown to cause least biochemical changes, not limiting pepsin hydrolysis. Loss of free amino groups, increase of fluorescence and brown colour of Ara h 1 glycated at 60 and 145 °C indicated advanced and final stages of MR. Non-treated Ara h 1 inhibited Caco-2 cell proliferation and stimulated IL-8 secretion. This effect was less pronounced for glycated Ara h 1. Incubation of Caco-2 cells with non-hydrolysed Ara h 1, glycated at the temperature of 37 and 60 °C, did not stimulate IL-8 secretion.
Conclusion
Each applied time/temperature-treatment combination caused different biochemical changes of Ara h 1, underlining diversity of formed MRPs. MR, independently of temperature/time conditions, reduced the pro-inflammatory properties of native Ara h 1, reflected in stimulation of IL-8 secretion from intestinal epithelial cells.
Original language | English |
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Pages (from-to) | 1927-1938 |
Journal | European Journal of Nutrition |
Volume | 52 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2013 |
Keywords
- reaction-products
- in-vitro
- colorimetric assay
- model system
- fluorescence
- proliferation
- allergens
- digestion
- antioxidant
- prevalence
Fingerprint
Dive into the research topics of 'Effect of Maillard reaction on biochemical properties of peanut 7S globulin (Ara h 1) and its interaction with a human colon cancer cell line (Caco-2)'. Together they form a unique fingerprint.Projects
- 2 Finished
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NETGREEN (KB-14-011-023, KB-14-003-041)
Brouwer, F. (Project Leader)
1/01/13 → 31/12/14
Project: LVVN project
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INFOGEST Digestie alternatieve eiwitten (KB-15-001-002)
Wichers, H. (Project Leader)
1/01/11 → 31/12/15
Project: LVVN project