Effect of H7N1 vaccination on highly pathogenic avian influenza H7N7 virus transmission in turkeys

M.E.H. Bos, M. Nielen, G. Koch, A. Stegeman, M.C.M. de Jong

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

This study describes the results of a transmission experiment with highly pathogenic avian influenza (HPAI) H7N7 virus in 12-week-old turkeys. Cloacal and tracheal swabs as well as serum samples were taken to monitor the infection both in inoculated and in susceptible contact turkeys, which were all either unvaccinated, vaccinated once or vaccinated twice with H7N1. Swabs were tested by real-time RT-PCR and serum samples with hemagglutination inhibition test (HI). Unvaccinated contact birds had a mean infectious period of 6.2 days, and an estimated transmission rate parameter of 1.26 per infectious bird per day. However, no virus shedding was found in inoculated vaccinated turkeys and thus we concluded that vaccination with H7N1 protected against challenge with HPAI H7N7 virus.
Original languageEnglish
Pages (from-to)6322-6328
JournalVaccine
Volume26
Issue number50
DOIs
Publication statusPublished - 2008

Fingerprint

H7N7 Subtype Influenza A Virus
Influenza in Birds
virus transmission
avian influenza
Orthomyxoviridae
Influenza A virus
Vaccination
vaccination
Birds
Hemagglutination Inhibition Tests
Virus Shedding
hemagglutination inhibition test
viral shedding
birds
Serum
Real-Time Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction
sampling
Infection
infection

Keywords

  • infection
  • chickens
  • susceptibility
  • patterns
  • efficacy
  • h5

Cite this

Bos, M.E.H. ; Nielen, M. ; Koch, G. ; Stegeman, A. ; de Jong, M.C.M. / Effect of H7N1 vaccination on highly pathogenic avian influenza H7N7 virus transmission in turkeys. In: Vaccine. 2008 ; Vol. 26, No. 50. pp. 6322-6328.
@article{6e7ee24bdca1466bb684c85513e3a66b,
title = "Effect of H7N1 vaccination on highly pathogenic avian influenza H7N7 virus transmission in turkeys",
abstract = "This study describes the results of a transmission experiment with highly pathogenic avian influenza (HPAI) H7N7 virus in 12-week-old turkeys. Cloacal and tracheal swabs as well as serum samples were taken to monitor the infection both in inoculated and in susceptible contact turkeys, which were all either unvaccinated, vaccinated once or vaccinated twice with H7N1. Swabs were tested by real-time RT-PCR and serum samples with hemagglutination inhibition test (HI). Unvaccinated contact birds had a mean infectious period of 6.2 days, and an estimated transmission rate parameter of 1.26 per infectious bird per day. However, no virus shedding was found in inoculated vaccinated turkeys and thus we concluded that vaccination with H7N1 protected against challenge with HPAI H7N7 virus.",
keywords = "infection, chickens, susceptibility, patterns, efficacy, h5",
author = "M.E.H. Bos and M. Nielen and G. Koch and A. Stegeman and {de Jong}, M.C.M.",
year = "2008",
doi = "10.1016/j.vaccine.2008.09.022",
language = "English",
volume = "26",
pages = "6322--6328",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier",
number = "50",

}

Effect of H7N1 vaccination on highly pathogenic avian influenza H7N7 virus transmission in turkeys. / Bos, M.E.H.; Nielen, M.; Koch, G.; Stegeman, A.; de Jong, M.C.M.

In: Vaccine, Vol. 26, No. 50, 2008, p. 6322-6328.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effect of H7N1 vaccination on highly pathogenic avian influenza H7N7 virus transmission in turkeys

AU - Bos, M.E.H.

AU - Nielen, M.

AU - Koch, G.

AU - Stegeman, A.

AU - de Jong, M.C.M.

PY - 2008

Y1 - 2008

N2 - This study describes the results of a transmission experiment with highly pathogenic avian influenza (HPAI) H7N7 virus in 12-week-old turkeys. Cloacal and tracheal swabs as well as serum samples were taken to monitor the infection both in inoculated and in susceptible contact turkeys, which were all either unvaccinated, vaccinated once or vaccinated twice with H7N1. Swabs were tested by real-time RT-PCR and serum samples with hemagglutination inhibition test (HI). Unvaccinated contact birds had a mean infectious period of 6.2 days, and an estimated transmission rate parameter of 1.26 per infectious bird per day. However, no virus shedding was found in inoculated vaccinated turkeys and thus we concluded that vaccination with H7N1 protected against challenge with HPAI H7N7 virus.

AB - This study describes the results of a transmission experiment with highly pathogenic avian influenza (HPAI) H7N7 virus in 12-week-old turkeys. Cloacal and tracheal swabs as well as serum samples were taken to monitor the infection both in inoculated and in susceptible contact turkeys, which were all either unvaccinated, vaccinated once or vaccinated twice with H7N1. Swabs were tested by real-time RT-PCR and serum samples with hemagglutination inhibition test (HI). Unvaccinated contact birds had a mean infectious period of 6.2 days, and an estimated transmission rate parameter of 1.26 per infectious bird per day. However, no virus shedding was found in inoculated vaccinated turkeys and thus we concluded that vaccination with H7N1 protected against challenge with HPAI H7N7 virus.

KW - infection

KW - chickens

KW - susceptibility

KW - patterns

KW - efficacy

KW - h5

U2 - 10.1016/j.vaccine.2008.09.022

DO - 10.1016/j.vaccine.2008.09.022

M3 - Article

VL - 26

SP - 6322

EP - 6328

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 50

ER -