Effect of growth conditions on the efficiency of cell disruption of Neochloris oleoabundans

C. Safi*, G. Olivieri, N. Engelen-Smit, W. Spekking, R. Veloo, L.A.M. van den Broek, L. Sijtsma

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The impact of four different growth conditions on the cell disruption efficiency of Neochloris oleoabundans was investigated. A mechanical and biological cell disruption methods were evaluated separately and combined. It has been established that microalgae grown in marine water under nitrogen deprivation were the most resistant against cell disruption methods and released the lowest amount of proteins. The release of lipids, however, followed the “hindered molecule diffusion phenomenon” because it did not follow the same release pattern as proteins. The enzymatic treatment was efficient enough to release the majority of the proteins without combining it with high-pressure homogenization. Regarding energy input, Neochloris oleoabundans grown in marine water under nitrogen deprivation required the highest energy input to release proteins (Ep = 13.76 kWh.kg−1) and to break the cells by high-pressure homogenization (Ex – HPH = 1.14 kWh.kg−1) or by the combination of enzymes and High-pressure homogenization (Ex – ENZ = 2.79 kWh.kg−1).

Original languageEnglish
Article number122699
JournalBioresource Technology
Volume300
DOIs
Publication statusPublished - 1 Mar 2020

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Proteins
protein
Nitrogen
Water
nitrogen
Lipids
energy
Enzymes
lipid
enzyme
water
Molecules
effect
method

Keywords

  • Cell disintegration
  • Enzymes
  • High-pressure homogenization
  • Nitrogen deplete
  • Nitrogen replete

Cite this

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title = "Effect of growth conditions on the efficiency of cell disruption of Neochloris oleoabundans",
abstract = "The impact of four different growth conditions on the cell disruption efficiency of Neochloris oleoabundans was investigated. A mechanical and biological cell disruption methods were evaluated separately and combined. It has been established that microalgae grown in marine water under nitrogen deprivation were the most resistant against cell disruption methods and released the lowest amount of proteins. The release of lipids, however, followed the “hindered molecule diffusion phenomenon” because it did not follow the same release pattern as proteins. The enzymatic treatment was efficient enough to release the majority of the proteins without combining it with high-pressure homogenization. Regarding energy input, Neochloris oleoabundans grown in marine water under nitrogen deprivation required the highest energy input to release proteins (Ep = 13.76 kWh.kg−1) and to break the cells by high-pressure homogenization (Ex – HPH = 1.14 kWh.kg−1) or by the combination of enzymes and High-pressure homogenization (Ex – ENZ = 2.79 kWh.kg−1).",
keywords = "Cell disintegration, Enzymes, High-pressure homogenization, Nitrogen deplete, Nitrogen replete",
author = "C. Safi and G. Olivieri and N. Engelen-Smit and W. Spekking and R. Veloo and {van den Broek}, L.A.M. and L. Sijtsma",
year = "2020",
month = "3",
day = "1",
doi = "10.1016/j.biortech.2019.122699",
language = "English",
volume = "300",
journal = "Bioresource Technology",
issn = "0960-8524",
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Effect of growth conditions on the efficiency of cell disruption of Neochloris oleoabundans. / Safi, C.; Olivieri, G.; Engelen-Smit, N.; Spekking, W.; Veloo, R.; van den Broek, L.A.M.; Sijtsma, L.

In: Bioresource Technology, Vol. 300, 122699, 01.03.2020.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effect of growth conditions on the efficiency of cell disruption of Neochloris oleoabundans

AU - Safi, C.

AU - Olivieri, G.

AU - Engelen-Smit, N.

AU - Spekking, W.

AU - Veloo, R.

AU - van den Broek, L.A.M.

AU - Sijtsma, L.

PY - 2020/3/1

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N2 - The impact of four different growth conditions on the cell disruption efficiency of Neochloris oleoabundans was investigated. A mechanical and biological cell disruption methods were evaluated separately and combined. It has been established that microalgae grown in marine water under nitrogen deprivation were the most resistant against cell disruption methods and released the lowest amount of proteins. The release of lipids, however, followed the “hindered molecule diffusion phenomenon” because it did not follow the same release pattern as proteins. The enzymatic treatment was efficient enough to release the majority of the proteins without combining it with high-pressure homogenization. Regarding energy input, Neochloris oleoabundans grown in marine water under nitrogen deprivation required the highest energy input to release proteins (Ep = 13.76 kWh.kg−1) and to break the cells by high-pressure homogenization (Ex – HPH = 1.14 kWh.kg−1) or by the combination of enzymes and High-pressure homogenization (Ex – ENZ = 2.79 kWh.kg−1).

AB - The impact of four different growth conditions on the cell disruption efficiency of Neochloris oleoabundans was investigated. A mechanical and biological cell disruption methods were evaluated separately and combined. It has been established that microalgae grown in marine water under nitrogen deprivation were the most resistant against cell disruption methods and released the lowest amount of proteins. The release of lipids, however, followed the “hindered molecule diffusion phenomenon” because it did not follow the same release pattern as proteins. The enzymatic treatment was efficient enough to release the majority of the proteins without combining it with high-pressure homogenization. Regarding energy input, Neochloris oleoabundans grown in marine water under nitrogen deprivation required the highest energy input to release proteins (Ep = 13.76 kWh.kg−1) and to break the cells by high-pressure homogenization (Ex – HPH = 1.14 kWh.kg−1) or by the combination of enzymes and High-pressure homogenization (Ex – ENZ = 2.79 kWh.kg−1).

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KW - High-pressure homogenization

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KW - Nitrogen replete

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