Effect of fertilizers on galanthamine and metabolite profiles in narcissus bulbs by 1H NMR

A. Lubbe, Y.H. Choi, P.J.M. Vreeburg, R. Verpoorte

    Research output: Contribution to journalArticleAcademicpeer-review

    24 Citations (Scopus)

    Abstract

    Narcissus bulbs contain the biologically active alkaloid galanthamine, and Narcissus is being developed as a natural source of the molecule for the pharmaceutical industry. The effect of fertilizer on galanthamine production was investigated in a field study using a 1H nuclear magnetic resonance (NMR) metabolite profiling approach. Galanthamine was quantitated and major metabolites in the bulbs were identified. The application of standard fertilization levels of nitrogen and potassium caused a significant increase in galanthamine as compared to a control. Multivariate data analysis of the 1H NMR data revealed that applying double the standard level of nitrogen fertilizer resulted in production of more amino acids and citric acid cycle intermediates, but not more galanthamine. The results indicated that standard levels of fertilizer currently applied in The Netherlands are sufficient for optimal galanthamine accumulation in the bulbs. This study shows how 1H NMR-based metabolic profiling can provide insight into the response of plant metabolism to agricultural practices.
    Original languageEnglish
    Pages (from-to)3155-3161
    JournalJournal of Agricultural and Food Chemistry
    Volume59
    Issue number7
    DOIs
    Publication statusPublished - 2011

    Fingerprint

    Narcissus
    Galantamine
    Fertilizers
    Metabolites
    bulbs
    nuclear magnetic resonance spectroscopy
    Magnetic Resonance Spectroscopy
    Nuclear magnetic resonance
    fertilizers
    metabolites
    tricarboxylic acid cycle
    metabolomics
    multivariate analysis
    plant response
    alkaloids
    Netherlands
    nitrogen fertilizers
    Nitrogen
    potassium
    Nitrogen fertilizers

    Keywords

    • amaryllidaceae alkaloids
    • nitrogen-fertilizer
    • gas-chromatography
    • nmr
    • growth
    • metabolomics
    • leaves

    Cite this

    Lubbe, A. ; Choi, Y.H. ; Vreeburg, P.J.M. ; Verpoorte, R. / Effect of fertilizers on galanthamine and metabolite profiles in narcissus bulbs by 1H NMR. In: Journal of Agricultural and Food Chemistry. 2011 ; Vol. 59, No. 7. pp. 3155-3161.
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    abstract = "Narcissus bulbs contain the biologically active alkaloid galanthamine, and Narcissus is being developed as a natural source of the molecule for the pharmaceutical industry. The effect of fertilizer on galanthamine production was investigated in a field study using a 1H nuclear magnetic resonance (NMR) metabolite profiling approach. Galanthamine was quantitated and major metabolites in the bulbs were identified. The application of standard fertilization levels of nitrogen and potassium caused a significant increase in galanthamine as compared to a control. Multivariate data analysis of the 1H NMR data revealed that applying double the standard level of nitrogen fertilizer resulted in production of more amino acids and citric acid cycle intermediates, but not more galanthamine. The results indicated that standard levels of fertilizer currently applied in The Netherlands are sufficient for optimal galanthamine accumulation in the bulbs. This study shows how 1H NMR-based metabolic profiling can provide insight into the response of plant metabolism to agricultural practices.",
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    Effect of fertilizers on galanthamine and metabolite profiles in narcissus bulbs by 1H NMR. / Lubbe, A.; Choi, Y.H.; Vreeburg, P.J.M.; Verpoorte, R.

    In: Journal of Agricultural and Food Chemistry, Vol. 59, No. 7, 2011, p. 3155-3161.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

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    AU - Lubbe, A.

    AU - Choi, Y.H.

    AU - Vreeburg, P.J.M.

    AU - Verpoorte, R.

    PY - 2011

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    AB - Narcissus bulbs contain the biologically active alkaloid galanthamine, and Narcissus is being developed as a natural source of the molecule for the pharmaceutical industry. The effect of fertilizer on galanthamine production was investigated in a field study using a 1H nuclear magnetic resonance (NMR) metabolite profiling approach. Galanthamine was quantitated and major metabolites in the bulbs were identified. The application of standard fertilization levels of nitrogen and potassium caused a significant increase in galanthamine as compared to a control. Multivariate data analysis of the 1H NMR data revealed that applying double the standard level of nitrogen fertilizer resulted in production of more amino acids and citric acid cycle intermediates, but not more galanthamine. The results indicated that standard levels of fertilizer currently applied in The Netherlands are sufficient for optimal galanthamine accumulation in the bulbs. This study shows how 1H NMR-based metabolic profiling can provide insight into the response of plant metabolism to agricultural practices.

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