Abstract
A series of in vitro experiments were completed to evaluate the potential of enzyme extracts, obtained from the white-rot fungi Trametes versicolor (TV1, TV2), Bjerkandera adusta (BA) and Fomes fomentarius (FF), to increase degradation of cell wall components of wheat straw. The studies were conducted as a completely randomized design and analysed using one-way ANOVA. Enzyme activities of the extracts, previously obtained from a liquid culture medium, were characterized in terms of laccase and peroxidase for ligninolytic activity. Carboxymethyl cellulase (CMCase) and avicell digesting cellulase (Avicelase) were used for cellulolytic enzyme assays. Wheat straw samples were incubated with enzyme extracts in a citrate buffer (pH 5.0) in a forced air oven at 25 °C for 6 days. In vitro NDF digestibility (IVNDFD), and the rate and extent of NDF fermentation, without and after incubation with the white-rot enzyme extracts, were determined using a gravimetric microbiological method and a gas production technique, respectively. Results from cell wall chemical composition showed that TV2 and BA enzyme extracts decreased NDF concentration (P
| Original language | English |
|---|---|
| Pages (from-to) | 326-338 |
| Journal | Animal Feed Science and Technology |
| Volume | 141 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | Published - 2008 |
Keywords
- volatile fatty-acids
- rumen microorganisms
- manganese peroxidase
- trametes-versicolor
- lignin degradation
- phenolic-acids
- rice straw
- fermentation
- fiber
- polysaccharides