Effect of diets fortified with tomatoes or onions with variable quercetin-glycoside content on azoxymethane-induced aberrant crypt foci in the colon of rats

A.P. Femia, G. Caderni, M. Ianni, M. Salvadori, E.G.W.M. Schijlen, G. Collins, A.G. Bovy, P. Dolara

    Research output: Contribution to journalArticleAcademicpeer-review

    25 Citations (Scopus)

    Abstract

    Background: Onion and tomato are vegetables widely consumed by humans and epidemiological studies show an inverse association between vegetable consumption and colon cancer risk; however, the effect on colon cancer of diets containing high levels of vegetables like onion and tomato are not clear. Aims of the study: To investigate whether tomatoes and onions,with low or high quercetin-glycoside content, could reduce azoxymethane (AOM)-induced Aberrant Crypt Foci (ACF), preneoplastic lesions in the colon of rats. Methods: Male Fisher 344 rats were fed the following diets: a) high fat (HF) diet (control diet); b) HF diet containing 20 % (w/w) tomatoes with a low quercetin-glycoside content (final concentration in the diet: 5 mg/kg of quercetin aglycone equivalents); c) HF diet containing 20% (w/w) high quercetin-glycoside tomatoes (100 mg/kg final concentration of quercetin aglycone equivalents); d) HF diet containing 20 % (w/w) low quercetin-glycoside onions (14 mg/kg of quercetin aglycone equivalents in the diet); e) HF diet containing 20 % (w/w) high quercetin-glycoside onions (360 mg/kg quercetin aglycone equivalents in the diet). After 2 wks of feeding, all rats were treated twice, 1 wk apart, with AOM (12 mg/kg, s. c.). The dietary treatments continued until sacrifice, 7 wks after the first injection with AOM. Results: ACF induction did not vary in animals fed low or high quercetin-glycoside tomatoes relative to controls. On the contrary, rats fed 20% (w/w) onion-based diets, with low or high quercetin-glycoside content, showed an increase in number, multiplicity and large ACF compared to the control group (number of ACF/colon 145 ± 15 (SE), 255 ± 11 and 218 ± 16 in controls, low and high-quercetin-glycoside groups, respectively; p <0.01). Proliferative activity of the colon did not vary between animals fed control and high quercetin-glycoside tomato diet. The height of the crypts in normal mucosa of rats fed high quercetinglycoside onions was significantly increased compared to control rats (cells/emicrypt 38.4 ± 1.2 (SE) and 41.3 ± 0.6 in controls and high quercetin-glycoside onions group, p <0.05). Conclusions: None of the diets supplemented with onion or tomato with variable quercetin-glycoside content demonstrated a potential chemopreventive effect on ACF-induction by AOM in rats
    Original languageEnglish
    Pages (from-to)346-352
    JournalEuropean Journal of Nutrition
    Volume42
    Issue number6
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    Aberrant Crypt Foci
    Azoxymethane
    Onions
    Quercetin
    Lycopersicon esculentum
    Glycosides
    Colon
    Diet
    High Fat Diet
    Vegetables
    Colonic Neoplasms

    Keywords

    • chemopreventive agents
    • f344 rats
    • cancer
    • carcinogenesis
    • consumption
    • acid
    • chalcone
    • rectum
    • fruit
    • rutin

    Cite this

    @article{038faccf4d63464287652e30e156d00d,
    title = "Effect of diets fortified with tomatoes or onions with variable quercetin-glycoside content on azoxymethane-induced aberrant crypt foci in the colon of rats",
    abstract = "Background: Onion and tomato are vegetables widely consumed by humans and epidemiological studies show an inverse association between vegetable consumption and colon cancer risk; however, the effect on colon cancer of diets containing high levels of vegetables like onion and tomato are not clear. Aims of the study: To investigate whether tomatoes and onions,with low or high quercetin-glycoside content, could reduce azoxymethane (AOM)-induced Aberrant Crypt Foci (ACF), preneoplastic lesions in the colon of rats. Methods: Male Fisher 344 rats were fed the following diets: a) high fat (HF) diet (control diet); b) HF diet containing 20 {\%} (w/w) tomatoes with a low quercetin-glycoside content (final concentration in the diet: 5 mg/kg of quercetin aglycone equivalents); c) HF diet containing 20{\%} (w/w) high quercetin-glycoside tomatoes (100 mg/kg final concentration of quercetin aglycone equivalents); d) HF diet containing 20 {\%} (w/w) low quercetin-glycoside onions (14 mg/kg of quercetin aglycone equivalents in the diet); e) HF diet containing 20 {\%} (w/w) high quercetin-glycoside onions (360 mg/kg quercetin aglycone equivalents in the diet). After 2 wks of feeding, all rats were treated twice, 1 wk apart, with AOM (12 mg/kg, s. c.). The dietary treatments continued until sacrifice, 7 wks after the first injection with AOM. Results: ACF induction did not vary in animals fed low or high quercetin-glycoside tomatoes relative to controls. On the contrary, rats fed 20{\%} (w/w) onion-based diets, with low or high quercetin-glycoside content, showed an increase in number, multiplicity and large ACF compared to the control group (number of ACF/colon 145 ± 15 (SE), 255 ± 11 and 218 ± 16 in controls, low and high-quercetin-glycoside groups, respectively; p <0.01). Proliferative activity of the colon did not vary between animals fed control and high quercetin-glycoside tomato diet. The height of the crypts in normal mucosa of rats fed high quercetinglycoside onions was significantly increased compared to control rats (cells/emicrypt 38.4 ± 1.2 (SE) and 41.3 ± 0.6 in controls and high quercetin-glycoside onions group, p <0.05). Conclusions: None of the diets supplemented with onion or tomato with variable quercetin-glycoside content demonstrated a potential chemopreventive effect on ACF-induction by AOM in rats",
    keywords = "chemopreventive agents, f344 rats, cancer, carcinogenesis, consumption, acid, chalcone, rectum, fruit, rutin",
    author = "A.P. Femia and G. Caderni and M. Ianni and M. Salvadori and E.G.W.M. Schijlen and G. Collins and A.G. Bovy and P. Dolara",
    year = "2003",
    doi = "10.1007/s00394-003-0431-5",
    language = "English",
    volume = "42",
    pages = "346--352",
    journal = "European Journal of Nutrition",
    issn = "1436-6207",
    publisher = "Springer Verlag",
    number = "6",

    }

    Effect of diets fortified with tomatoes or onions with variable quercetin-glycoside content on azoxymethane-induced aberrant crypt foci in the colon of rats. / Femia, A.P.; Caderni, G.; Ianni, M.; Salvadori, M.; Schijlen, E.G.W.M.; Collins, G.; Bovy, A.G.; Dolara, P.

    In: European Journal of Nutrition, Vol. 42, No. 6, 2003, p. 346-352.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Effect of diets fortified with tomatoes or onions with variable quercetin-glycoside content on azoxymethane-induced aberrant crypt foci in the colon of rats

    AU - Femia, A.P.

    AU - Caderni, G.

    AU - Ianni, M.

    AU - Salvadori, M.

    AU - Schijlen, E.G.W.M.

    AU - Collins, G.

    AU - Bovy, A.G.

    AU - Dolara, P.

    PY - 2003

    Y1 - 2003

    N2 - Background: Onion and tomato are vegetables widely consumed by humans and epidemiological studies show an inverse association between vegetable consumption and colon cancer risk; however, the effect on colon cancer of diets containing high levels of vegetables like onion and tomato are not clear. Aims of the study: To investigate whether tomatoes and onions,with low or high quercetin-glycoside content, could reduce azoxymethane (AOM)-induced Aberrant Crypt Foci (ACF), preneoplastic lesions in the colon of rats. Methods: Male Fisher 344 rats were fed the following diets: a) high fat (HF) diet (control diet); b) HF diet containing 20 % (w/w) tomatoes with a low quercetin-glycoside content (final concentration in the diet: 5 mg/kg of quercetin aglycone equivalents); c) HF diet containing 20% (w/w) high quercetin-glycoside tomatoes (100 mg/kg final concentration of quercetin aglycone equivalents); d) HF diet containing 20 % (w/w) low quercetin-glycoside onions (14 mg/kg of quercetin aglycone equivalents in the diet); e) HF diet containing 20 % (w/w) high quercetin-glycoside onions (360 mg/kg quercetin aglycone equivalents in the diet). After 2 wks of feeding, all rats were treated twice, 1 wk apart, with AOM (12 mg/kg, s. c.). The dietary treatments continued until sacrifice, 7 wks after the first injection with AOM. Results: ACF induction did not vary in animals fed low or high quercetin-glycoside tomatoes relative to controls. On the contrary, rats fed 20% (w/w) onion-based diets, with low or high quercetin-glycoside content, showed an increase in number, multiplicity and large ACF compared to the control group (number of ACF/colon 145 ± 15 (SE), 255 ± 11 and 218 ± 16 in controls, low and high-quercetin-glycoside groups, respectively; p <0.01). Proliferative activity of the colon did not vary between animals fed control and high quercetin-glycoside tomato diet. The height of the crypts in normal mucosa of rats fed high quercetinglycoside onions was significantly increased compared to control rats (cells/emicrypt 38.4 ± 1.2 (SE) and 41.3 ± 0.6 in controls and high quercetin-glycoside onions group, p <0.05). Conclusions: None of the diets supplemented with onion or tomato with variable quercetin-glycoside content demonstrated a potential chemopreventive effect on ACF-induction by AOM in rats

    AB - Background: Onion and tomato are vegetables widely consumed by humans and epidemiological studies show an inverse association between vegetable consumption and colon cancer risk; however, the effect on colon cancer of diets containing high levels of vegetables like onion and tomato are not clear. Aims of the study: To investigate whether tomatoes and onions,with low or high quercetin-glycoside content, could reduce azoxymethane (AOM)-induced Aberrant Crypt Foci (ACF), preneoplastic lesions in the colon of rats. Methods: Male Fisher 344 rats were fed the following diets: a) high fat (HF) diet (control diet); b) HF diet containing 20 % (w/w) tomatoes with a low quercetin-glycoside content (final concentration in the diet: 5 mg/kg of quercetin aglycone equivalents); c) HF diet containing 20% (w/w) high quercetin-glycoside tomatoes (100 mg/kg final concentration of quercetin aglycone equivalents); d) HF diet containing 20 % (w/w) low quercetin-glycoside onions (14 mg/kg of quercetin aglycone equivalents in the diet); e) HF diet containing 20 % (w/w) high quercetin-glycoside onions (360 mg/kg quercetin aglycone equivalents in the diet). After 2 wks of feeding, all rats were treated twice, 1 wk apart, with AOM (12 mg/kg, s. c.). The dietary treatments continued until sacrifice, 7 wks after the first injection with AOM. Results: ACF induction did not vary in animals fed low or high quercetin-glycoside tomatoes relative to controls. On the contrary, rats fed 20% (w/w) onion-based diets, with low or high quercetin-glycoside content, showed an increase in number, multiplicity and large ACF compared to the control group (number of ACF/colon 145 ± 15 (SE), 255 ± 11 and 218 ± 16 in controls, low and high-quercetin-glycoside groups, respectively; p <0.01). Proliferative activity of the colon did not vary between animals fed control and high quercetin-glycoside tomato diet. The height of the crypts in normal mucosa of rats fed high quercetinglycoside onions was significantly increased compared to control rats (cells/emicrypt 38.4 ± 1.2 (SE) and 41.3 ± 0.6 in controls and high quercetin-glycoside onions group, p <0.05). Conclusions: None of the diets supplemented with onion or tomato with variable quercetin-glycoside content demonstrated a potential chemopreventive effect on ACF-induction by AOM in rats

    KW - chemopreventive agents

    KW - f344 rats

    KW - cancer

    KW - carcinogenesis

    KW - consumption

    KW - acid

    KW - chalcone

    KW - rectum

    KW - fruit

    KW - rutin

    U2 - 10.1007/s00394-003-0431-5

    DO - 10.1007/s00394-003-0431-5

    M3 - Article

    VL - 42

    SP - 346

    EP - 352

    JO - European Journal of Nutrition

    JF - European Journal of Nutrition

    SN - 1436-6207

    IS - 6

    ER -