TY - JOUR
T1 - Effect of dietary administration of probiotics on growth and intestine functionality of juvenile Senegalese sole (Solea senegalensis, Kaup 1858)
AU - Saenz de Rodriganez, M.A.
AU - Diaz-Rosales, P.
AU - Chabrillon, M.
AU - Smidt, H.
AU - Arijo, S.
AU - Leon-Rubio, J.M.
AU - Alarcon, F.J.
AU - Balebona, M.C.
AU - Morinigo, M.A.
AU - Cara, J.B.
AU - Moyano, F.J.
PY - 2009
Y1 - 2009
N2 - The effects of the dietary administration of two bacterial probiotic strains (Ppd11 and Pdp13) from the Alteromonadaceae family for 60 days, were assessed by measuring growth and feed efficiency, activities of leucine aminopeptidase and alkaline phosphatase and structural changes in the intestine of juvenile Senegalese sole. In addition, the profile of intestinal microbiota was studied by Denaturing Gradient Gel Electrophoresis. Growth and nutrient utilization were significantly higher in fish receiving probiotics than in those fed the control diet. No differences were observed in proximal composition between treatments, though higher lipid muscle content was measured in fish receiving Pdp13. Those fish also exhibited higher activities of AP when compared to Ppd11 and control groups. The profile of intestinal microbiota clearly separated those fish receiving probiotics from those of the control group. Microscopical examination revealed accumulation of lipid droplets in the enterocytes of fish receiving the control diet, but not in those fed on probiotics. Interactions between those structural changes and growth performance are discussed
AB - The effects of the dietary administration of two bacterial probiotic strains (Ppd11 and Pdp13) from the Alteromonadaceae family for 60 days, were assessed by measuring growth and feed efficiency, activities of leucine aminopeptidase and alkaline phosphatase and structural changes in the intestine of juvenile Senegalese sole. In addition, the profile of intestinal microbiota was studied by Denaturing Gradient Gel Electrophoresis. Growth and nutrient utilization were significantly higher in fish receiving probiotics than in those fed the control diet. No differences were observed in proximal composition between treatments, though higher lipid muscle content was measured in fish receiving Pdp13. Those fish also exhibited higher activities of AP when compared to Ppd11 and control groups. The profile of intestinal microbiota clearly separated those fish receiving probiotics from those of the control group. Microscopical examination revealed accumulation of lipid droplets in the enterocytes of fish receiving the control diet, but not in those fed on probiotics. Interactions between those structural changes and growth performance are discussed
KW - trout oncorhynchus-mykiss
KW - gel-electrophoresis analysis
KW - bass dicentrarchus-labrax
KW - damselae subsp-piscicida
KW - innate immune-responses
KW - sparus-aurata l.
KW - rainbow-trout
KW - scophthalmus-maximus
KW - lipid sources
KW - aquaculture
U2 - 10.1111/j.1365-2095.2008.00581.x
DO - 10.1111/j.1365-2095.2008.00581.x
M3 - Article
SN - 1353-5773
VL - 15
SP - 177
EP - 185
JO - Aquaculture Nutrition
JF - Aquaculture Nutrition
IS - 2
ER -