Effect of cell wall characteristics on algae nutrient digestibility in Nile tilapia (Oreochromis niloticus) and African catfish (Clarus gariepinus)

Emma Teuling, Johan W. Schrama*, Harry Gruppen, Peter A. Wierenga

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

This study aimed to assess the effect of cell wall hardness and fish species on digestibility of unicellular sources. The gross composition, and the composition and cell wall hardness of the sources were determined for four sources. These were 3 microalgae species (Chlorella vulgaris, Scenedesmus dimorphus and Nannochloropsis gaditana) and a cyanobacterium (Arthrospira maxima). Apparent digestibility coefficients (ADCs) of their nutrients were determined in Nile tilapia and African catfish, at a 30% diet inclusion level. It was hypothesized that herbivores can access and thus digest unicellular proteins better than omnivores, and that the differences in protein digestion between the fish species increase with the robustness of the cell walls. Differences in cell wall hardness were quantified as the cells' resistance to mechanical shear. A. maxima was least resistant to shear: the time needed to disrupt 50% of the cells was 2 min compared to 24–33 min for the other sources. Differences were also measured in nutrient digestibility between the sources in both fish species. Contrary to the basal diet, which was digested differently between the fish species, there was no fish species effect on nutrient ADCs of the unicellular sources. A. maxima had the highest protein ADCs in both fish species (81.4–82.5%), followed by C. vulgaris (80.7–80.9%), N. gaditana (72.4–74.7%) and S. dimorphus (67.0–68.3%). Ingredient fat ADCs ranged between 65.1 and 89.1%. Unicellular non-starch polysaccharides (NSP), comprising the unicellular cell wall fraction, was not inert in both fish species (ADC > 46.0%), which was attributed to fermentation. The digestibility data suggest that the differences in nutrient accessibility of unicellular sources are dominant over the differences in digestive systems between herbivorous and omnivorous fish. Nevertheless, nutrient digestibility of the unicellular sources did not relate to the mechanical cell wall hardness.

Original languageEnglish
Pages (from-to)490-500
JournalAquaculture
Volume479
DOIs
Publication statusPublished - 1 Oct 2017

Fingerprint

algae
digestibility
Oreochromis niloticus
catfish
alga
cell walls
nutrient
Spirulina maxima
nutrients
fish
hardness
Chlorella vulgaris
shears
protein
Nannochloropsis
diet
Scenedesmus
effect
digestive system
omnivores

Keywords

  • Arthrospira (spirulina) maxima
  • Chlorella vulgaris
  • Nannochloropsis gaditana
  • Non-starch polysaccharides
  • Nutritional value
  • Scenedesmus dimorphus

Cite this

@article{9da264c5a423412db519c651df7b3732,
title = "Effect of cell wall characteristics on algae nutrient digestibility in Nile tilapia (Oreochromis niloticus) and African catfish (Clarus gariepinus)",
abstract = "This study aimed to assess the effect of cell wall hardness and fish species on digestibility of unicellular sources. The gross composition, and the composition and cell wall hardness of the sources were determined for four sources. These were 3 microalgae species (Chlorella vulgaris, Scenedesmus dimorphus and Nannochloropsis gaditana) and a cyanobacterium (Arthrospira maxima). Apparent digestibility coefficients (ADCs) of their nutrients were determined in Nile tilapia and African catfish, at a 30{\%} diet inclusion level. It was hypothesized that herbivores can access and thus digest unicellular proteins better than omnivores, and that the differences in protein digestion between the fish species increase with the robustness of the cell walls. Differences in cell wall hardness were quantified as the cells' resistance to mechanical shear. A. maxima was least resistant to shear: the time needed to disrupt 50{\%} of the cells was 2 min compared to 24–33 min for the other sources. Differences were also measured in nutrient digestibility between the sources in both fish species. Contrary to the basal diet, which was digested differently between the fish species, there was no fish species effect on nutrient ADCs of the unicellular sources. A. maxima had the highest protein ADCs in both fish species (81.4–82.5{\%}), followed by C. vulgaris (80.7–80.9{\%}), N. gaditana (72.4–74.7{\%}) and S. dimorphus (67.0–68.3{\%}). Ingredient fat ADCs ranged between 65.1 and 89.1{\%}. Unicellular non-starch polysaccharides (NSP), comprising the unicellular cell wall fraction, was not inert in both fish species (ADC > 46.0{\%}), which was attributed to fermentation. The digestibility data suggest that the differences in nutrient accessibility of unicellular sources are dominant over the differences in digestive systems between herbivorous and omnivorous fish. Nevertheless, nutrient digestibility of the unicellular sources did not relate to the mechanical cell wall hardness.",
keywords = "Arthrospira (spirulina) maxima, Chlorella vulgaris, Nannochloropsis gaditana, Non-starch polysaccharides, Nutritional value, Scenedesmus dimorphus",
author = "Emma Teuling and Schrama, {Johan W.} and Harry Gruppen and Wierenga, {Peter A.}",
year = "2017",
month = "10",
day = "1",
doi = "10.1016/j.aquaculture.2017.06.025",
language = "English",
volume = "479",
pages = "490--500",
journal = "Aquaculture",
issn = "0044-8486",
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Effect of cell wall characteristics on algae nutrient digestibility in Nile tilapia (Oreochromis niloticus) and African catfish (Clarus gariepinus). / Teuling, Emma; Schrama, Johan W.; Gruppen, Harry; Wierenga, Peter A.

In: Aquaculture, Vol. 479, 01.10.2017, p. 490-500.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effect of cell wall characteristics on algae nutrient digestibility in Nile tilapia (Oreochromis niloticus) and African catfish (Clarus gariepinus)

AU - Teuling, Emma

AU - Schrama, Johan W.

AU - Gruppen, Harry

AU - Wierenga, Peter A.

PY - 2017/10/1

Y1 - 2017/10/1

N2 - This study aimed to assess the effect of cell wall hardness and fish species on digestibility of unicellular sources. The gross composition, and the composition and cell wall hardness of the sources were determined for four sources. These were 3 microalgae species (Chlorella vulgaris, Scenedesmus dimorphus and Nannochloropsis gaditana) and a cyanobacterium (Arthrospira maxima). Apparent digestibility coefficients (ADCs) of their nutrients were determined in Nile tilapia and African catfish, at a 30% diet inclusion level. It was hypothesized that herbivores can access and thus digest unicellular proteins better than omnivores, and that the differences in protein digestion between the fish species increase with the robustness of the cell walls. Differences in cell wall hardness were quantified as the cells' resistance to mechanical shear. A. maxima was least resistant to shear: the time needed to disrupt 50% of the cells was 2 min compared to 24–33 min for the other sources. Differences were also measured in nutrient digestibility between the sources in both fish species. Contrary to the basal diet, which was digested differently between the fish species, there was no fish species effect on nutrient ADCs of the unicellular sources. A. maxima had the highest protein ADCs in both fish species (81.4–82.5%), followed by C. vulgaris (80.7–80.9%), N. gaditana (72.4–74.7%) and S. dimorphus (67.0–68.3%). Ingredient fat ADCs ranged between 65.1 and 89.1%. Unicellular non-starch polysaccharides (NSP), comprising the unicellular cell wall fraction, was not inert in both fish species (ADC > 46.0%), which was attributed to fermentation. The digestibility data suggest that the differences in nutrient accessibility of unicellular sources are dominant over the differences in digestive systems between herbivorous and omnivorous fish. Nevertheless, nutrient digestibility of the unicellular sources did not relate to the mechanical cell wall hardness.

AB - This study aimed to assess the effect of cell wall hardness and fish species on digestibility of unicellular sources. The gross composition, and the composition and cell wall hardness of the sources were determined for four sources. These were 3 microalgae species (Chlorella vulgaris, Scenedesmus dimorphus and Nannochloropsis gaditana) and a cyanobacterium (Arthrospira maxima). Apparent digestibility coefficients (ADCs) of their nutrients were determined in Nile tilapia and African catfish, at a 30% diet inclusion level. It was hypothesized that herbivores can access and thus digest unicellular proteins better than omnivores, and that the differences in protein digestion between the fish species increase with the robustness of the cell walls. Differences in cell wall hardness were quantified as the cells' resistance to mechanical shear. A. maxima was least resistant to shear: the time needed to disrupt 50% of the cells was 2 min compared to 24–33 min for the other sources. Differences were also measured in nutrient digestibility between the sources in both fish species. Contrary to the basal diet, which was digested differently between the fish species, there was no fish species effect on nutrient ADCs of the unicellular sources. A. maxima had the highest protein ADCs in both fish species (81.4–82.5%), followed by C. vulgaris (80.7–80.9%), N. gaditana (72.4–74.7%) and S. dimorphus (67.0–68.3%). Ingredient fat ADCs ranged between 65.1 and 89.1%. Unicellular non-starch polysaccharides (NSP), comprising the unicellular cell wall fraction, was not inert in both fish species (ADC > 46.0%), which was attributed to fermentation. The digestibility data suggest that the differences in nutrient accessibility of unicellular sources are dominant over the differences in digestive systems between herbivorous and omnivorous fish. Nevertheless, nutrient digestibility of the unicellular sources did not relate to the mechanical cell wall hardness.

KW - Arthrospira (spirulina) maxima

KW - Chlorella vulgaris

KW - Nannochloropsis gaditana

KW - Non-starch polysaccharides

KW - Nutritional value

KW - Scenedesmus dimorphus

U2 - 10.1016/j.aquaculture.2017.06.025

DO - 10.1016/j.aquaculture.2017.06.025

M3 - Article

VL - 479

SP - 490

EP - 500

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

ER -