Two groups of organic substances could be separated by hot ethanolic extraction, one coming out of solution after cooling, the other remaining in solution. From the solution a crystalline substance could be obtained, afterwards called 'Gliedine', causing disease symptoms after application to peas and oats. At minute concentrations it stimulated growth of oats. The residue caused grey speck disease in oats.
Fertilizer trials with differently diseased soils showed that only compost or CuSO4 could restore the soil (also in the 2nd year).
As 'Gliedine' slowly evaporated at 100 'C diseased soils were heated 3 h per day for 3 days, resulting in restoration of slightly diseased soils, almost cure of moderately diseased soils, and delay of disease symptoms on very diseased soils.
The curing effect Of CUS04 must very probably be ascribed to the formation of an insoluble compound with 'Gliedine'
|Qualification||Doctor of Philosophy|
|Award date||31 May 1927|
|Place of Publication||Wageningen|
|Publication status||Published - 1927|
- nutrient deficiencies
- plant nutrition