Ectopic expression of LLAG1, an AGAMOUS homologue from lily (Lilium longiflorum Thunb.) causes floral homeotic modifications in Arabidopsis.

V.A. Benedito, P.B. Visser, J.M. van Tuyl, G.C. Angenent, S.C. de Vries, F.A. Krens

Research output: Contribution to journalArticleAcademicpeer-review

41 Citations (Scopus)

Abstract

The ABC model for floral development was proposed more than 10 years ago and since then many studies have been performed on model species, such as Arabidopsis thaliana, Antirrhinum majus, and many other species in order to confirm this hypothesis. This led to additional information on flower development and to more complex molecular models. AGAMOUS (AG) is the only C type gene in Arabidopsis and it is responsible for stamen and carpel development as well as floral determinacy. LLAG1, an AG homologue from lily (Lilium longiflorum Thunb.) was isolated by screening a cDNA library derived from developing floral buds. The deduced amino acid sequence revealed the MIKC structure and a high homology in the MADS-box among AG and other orthologues. Phylogenetic analysis indicated a close relationship between LLAG1 and AG orthologues from monocot species. Spatial expression data showed LLAG1 transcripts exclusively in stamens and carpels, constituting the C domain of the ABC model. Functional analysis was carried out in Arabidopsis by overexpression of LLAG1 driven by the CaMV35S promoter. Transformed plants showed homeotic changes in the two outer floral whorls with some plants presenting the second whorl completely converted into stamens. Altogether, these data strongly indicated the functional homology between LLAG1 and AG.
Original languageEnglish
Pages (from-to)1391-1399
JournalJournal of Experimental Botany
Volume55
Issue number401
DOIs
Publication statusPublished - 2004

Fingerprint

Lilium
Lilium longiflorum
stamens
Arabidopsis
carpels
flowering
Antirrhinum
Antirrhinum majus
molecular models
Molecular Models
Liliopsida
Gene Library
cDNA libraries
Amino Acid Sequence
amino acid sequences
Arabidopsis thaliana
buds
promoter regions
screening
phylogeny

Keywords

  • mads-box genes
  • controlling flower development
  • protein-protein interactions
  • transcription factors
  • organ identity
  • gerbera-hybrida
  • plant biology
  • wild-type
  • petunia
  • transformation

Cite this

@article{ccfc2c6ff2774d42bb90795a9f2e0c69,
title = "Ectopic expression of LLAG1, an AGAMOUS homologue from lily (Lilium longiflorum Thunb.) causes floral homeotic modifications in Arabidopsis.",
abstract = "The ABC model for floral development was proposed more than 10 years ago and since then many studies have been performed on model species, such as Arabidopsis thaliana, Antirrhinum majus, and many other species in order to confirm this hypothesis. This led to additional information on flower development and to more complex molecular models. AGAMOUS (AG) is the only C type gene in Arabidopsis and it is responsible for stamen and carpel development as well as floral determinacy. LLAG1, an AG homologue from lily (Lilium longiflorum Thunb.) was isolated by screening a cDNA library derived from developing floral buds. The deduced amino acid sequence revealed the MIKC structure and a high homology in the MADS-box among AG and other orthologues. Phylogenetic analysis indicated a close relationship between LLAG1 and AG orthologues from monocot species. Spatial expression data showed LLAG1 transcripts exclusively in stamens and carpels, constituting the C domain of the ABC model. Functional analysis was carried out in Arabidopsis by overexpression of LLAG1 driven by the CaMV35S promoter. Transformed plants showed homeotic changes in the two outer floral whorls with some plants presenting the second whorl completely converted into stamens. Altogether, these data strongly indicated the functional homology between LLAG1 and AG.",
keywords = "mads-box genes, controlling flower development, protein-protein interactions, transcription factors, organ identity, gerbera-hybrida, plant biology, wild-type, petunia, transformation",
author = "V.A. Benedito and P.B. Visser and {van Tuyl}, J.M. and G.C. Angenent and {de Vries}, S.C. and F.A. Krens",
year = "2004",
doi = "10.1093/jxb/erh156",
language = "English",
volume = "55",
pages = "1391--1399",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "401",

}

Ectopic expression of LLAG1, an AGAMOUS homologue from lily (Lilium longiflorum Thunb.) causes floral homeotic modifications in Arabidopsis. / Benedito, V.A.; Visser, P.B.; van Tuyl, J.M.; Angenent, G.C.; de Vries, S.C.; Krens, F.A.

In: Journal of Experimental Botany, Vol. 55, No. 401, 2004, p. 1391-1399.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Ectopic expression of LLAG1, an AGAMOUS homologue from lily (Lilium longiflorum Thunb.) causes floral homeotic modifications in Arabidopsis.

AU - Benedito, V.A.

AU - Visser, P.B.

AU - van Tuyl, J.M.

AU - Angenent, G.C.

AU - de Vries, S.C.

AU - Krens, F.A.

PY - 2004

Y1 - 2004

N2 - The ABC model for floral development was proposed more than 10 years ago and since then many studies have been performed on model species, such as Arabidopsis thaliana, Antirrhinum majus, and many other species in order to confirm this hypothesis. This led to additional information on flower development and to more complex molecular models. AGAMOUS (AG) is the only C type gene in Arabidopsis and it is responsible for stamen and carpel development as well as floral determinacy. LLAG1, an AG homologue from lily (Lilium longiflorum Thunb.) was isolated by screening a cDNA library derived from developing floral buds. The deduced amino acid sequence revealed the MIKC structure and a high homology in the MADS-box among AG and other orthologues. Phylogenetic analysis indicated a close relationship between LLAG1 and AG orthologues from monocot species. Spatial expression data showed LLAG1 transcripts exclusively in stamens and carpels, constituting the C domain of the ABC model. Functional analysis was carried out in Arabidopsis by overexpression of LLAG1 driven by the CaMV35S promoter. Transformed plants showed homeotic changes in the two outer floral whorls with some plants presenting the second whorl completely converted into stamens. Altogether, these data strongly indicated the functional homology between LLAG1 and AG.

AB - The ABC model for floral development was proposed more than 10 years ago and since then many studies have been performed on model species, such as Arabidopsis thaliana, Antirrhinum majus, and many other species in order to confirm this hypothesis. This led to additional information on flower development and to more complex molecular models. AGAMOUS (AG) is the only C type gene in Arabidopsis and it is responsible for stamen and carpel development as well as floral determinacy. LLAG1, an AG homologue from lily (Lilium longiflorum Thunb.) was isolated by screening a cDNA library derived from developing floral buds. The deduced amino acid sequence revealed the MIKC structure and a high homology in the MADS-box among AG and other orthologues. Phylogenetic analysis indicated a close relationship between LLAG1 and AG orthologues from monocot species. Spatial expression data showed LLAG1 transcripts exclusively in stamens and carpels, constituting the C domain of the ABC model. Functional analysis was carried out in Arabidopsis by overexpression of LLAG1 driven by the CaMV35S promoter. Transformed plants showed homeotic changes in the two outer floral whorls with some plants presenting the second whorl completely converted into stamens. Altogether, these data strongly indicated the functional homology between LLAG1 and AG.

KW - mads-box genes

KW - controlling flower development

KW - protein-protein interactions

KW - transcription factors

KW - organ identity

KW - gerbera-hybrida

KW - plant biology

KW - wild-type

KW - petunia

KW - transformation

U2 - 10.1093/jxb/erh156

DO - 10.1093/jxb/erh156

M3 - Article

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EP - 1399

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

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ER -