Dynamics of the actin cytoskeleton in Phytophthora infestans

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

The actin cytoskeleton is conserved among all eukaryotes and plays essential roles during many cellular processes. It forms an internal framework in cells that is both dynamic and well organised. The plethora of functions ranges from facilitating cytoplasmic streaming, muscle contraction, formation of contractile rings, nuclear segregation, endocytosis and facilitating apical cell expansions. Oomycetes are filamentous organisms that resemble Fungi but are not related to Fungi. The two groups show significant structural, biochemical and genetic differences. One prominent lineage within the class of oomycetes is the genus Phytophthora. This genus comprises over 100 species that are all devastating plant pathogens threatening agriculture and natural environments. The potato late blight pathogen Phytophthora infestans was responsible for the Irish potato famine and remains a major threat today. Previously the actin organization has been studied in several oomycetes. Next to the common F-actin filaments and cables, cortical F-actin containing patches or plaques have been observed as in Fungi. However, only a static view was obtained. Here, we use an in vivo actin binding moiety labelled to a fluorescent group to investigate the actin cytoskeleton dynamics in hyphae of P. infestans. Our results provide the first visualisation of the dynamic reorganization of the actin cytoskeleton in oomycetes. In the future, this line will provide insight in the role of the actin cytoskeleton during infection.
Original languageEnglish
Title of host publicationBook of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013
Pages42
Publication statusPublished - 2013
EventOomycete Molecular Genetics Network Meeting 2013 - Blacksburg, United States
Duration: 10 Mar 201312 Mar 2013

Conference

ConferenceOomycete Molecular Genetics Network Meeting 2013
CountryUnited States
CityBlacksburg
Period10/03/1312/03/13

Fingerprint

Phytophthora infestans
microfilaments
Oomycetes
actin
fungi
cytoplasmic streaming
muscle contraction
endocytosis
Phytophthora
plant pathogens
hyphae
eukaryotic cells
cells
agriculture
pathogens
organisms
infection

Cite this

Meijer, H. J. G., Hua, C., Kots, K., Ketelaar, T., & Govers, F. (2013). Dynamics of the actin cytoskeleton in Phytophthora infestans. In Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013 (pp. 42)
Meijer, H.J.G. ; Hua, C. ; Kots, K. ; Ketelaar, T. ; Govers, F. / Dynamics of the actin cytoskeleton in Phytophthora infestans. Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013. 2013. pp. 42
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Meijer, HJG, Hua, C, Kots, K, Ketelaar, T & Govers, F 2013, Dynamics of the actin cytoskeleton in Phytophthora infestans. in Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013. pp. 42, Oomycete Molecular Genetics Network Meeting 2013, Blacksburg, United States, 10/03/13.

Dynamics of the actin cytoskeleton in Phytophthora infestans. / Meijer, H.J.G.; Hua, C.; Kots, K.; Ketelaar, T.; Govers, F.

Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013. 2013. p. 42.

Research output: Chapter in Book/Report/Conference proceedingAbstract

TY - CHAP

T1 - Dynamics of the actin cytoskeleton in Phytophthora infestans

AU - Meijer, H.J.G.

AU - Hua, C.

AU - Kots, K.

AU - Ketelaar, T.

AU - Govers, F.

PY - 2013

Y1 - 2013

N2 - The actin cytoskeleton is conserved among all eukaryotes and plays essential roles during many cellular processes. It forms an internal framework in cells that is both dynamic and well organised. The plethora of functions ranges from facilitating cytoplasmic streaming, muscle contraction, formation of contractile rings, nuclear segregation, endocytosis and facilitating apical cell expansions. Oomycetes are filamentous organisms that resemble Fungi but are not related to Fungi. The two groups show significant structural, biochemical and genetic differences. One prominent lineage within the class of oomycetes is the genus Phytophthora. This genus comprises over 100 species that are all devastating plant pathogens threatening agriculture and natural environments. The potato late blight pathogen Phytophthora infestans was responsible for the Irish potato famine and remains a major threat today. Previously the actin organization has been studied in several oomycetes. Next to the common F-actin filaments and cables, cortical F-actin containing patches or plaques have been observed as in Fungi. However, only a static view was obtained. Here, we use an in vivo actin binding moiety labelled to a fluorescent group to investigate the actin cytoskeleton dynamics in hyphae of P. infestans. Our results provide the first visualisation of the dynamic reorganization of the actin cytoskeleton in oomycetes. In the future, this line will provide insight in the role of the actin cytoskeleton during infection.

AB - The actin cytoskeleton is conserved among all eukaryotes and plays essential roles during many cellular processes. It forms an internal framework in cells that is both dynamic and well organised. The plethora of functions ranges from facilitating cytoplasmic streaming, muscle contraction, formation of contractile rings, nuclear segregation, endocytosis and facilitating apical cell expansions. Oomycetes are filamentous organisms that resemble Fungi but are not related to Fungi. The two groups show significant structural, biochemical and genetic differences. One prominent lineage within the class of oomycetes is the genus Phytophthora. This genus comprises over 100 species that are all devastating plant pathogens threatening agriculture and natural environments. The potato late blight pathogen Phytophthora infestans was responsible for the Irish potato famine and remains a major threat today. Previously the actin organization has been studied in several oomycetes. Next to the common F-actin filaments and cables, cortical F-actin containing patches or plaques have been observed as in Fungi. However, only a static view was obtained. Here, we use an in vivo actin binding moiety labelled to a fluorescent group to investigate the actin cytoskeleton dynamics in hyphae of P. infestans. Our results provide the first visualisation of the dynamic reorganization of the actin cytoskeleton in oomycetes. In the future, this line will provide insight in the role of the actin cytoskeleton during infection.

M3 - Abstract

SP - 42

BT - Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013

ER -

Meijer HJG, Hua C, Kots K, Ketelaar T, Govers F. Dynamics of the actin cytoskeleton in Phytophthora infestans. In Book of Abstracts Oomycete Molecular Genetics Network Meeting, Pacific Grove, California, USA, 10-12 March 2013. 2013. p. 42