Dynamics of archaeal community in soil with application of composted tannery sludge

Ana Roberta Lima Miranda, Lucas William Mendes, Leandro Nascimento Lemos, Jadson Emanuel Lopes Antunes, Marineide Rodrigues Amorim, Vania Maria Maciel Melo, Wanderley Jose de Melo, Paul J. van den Brink, Ademir Sergio Ferreira Araujo

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Application of composted tannery sludge (CTS) could promote a shift in the structure of soil microbial communities. Although the effect of CTS on bacterial community has been studied, it is unclear how the composition and diversity of archaeal community respond to CTS amendment and which environmental factors drive the community over time. Here, we hypothesize that the Archaea structure and composition respond to CTS amendment over the time. CTS had been previously applied annually along 6 years and this assessment occurred for 180 days following the application in the 7 th year by using different rates (0, 2.5, 5, 10 and 20 ton ha −1 ). We used amplicon 16S rRNA sequencing to assess the changes in the structure of the archaeal community. Thaumarchaeota and Euryarchaeota were the most abundant phyla found in soils with application of CTS, with Thaumarchaeota dominating the sequences in all samples with relative abundances of >98%. We observed a decreasing trend on the archaeal diversity over the time with increasing CTS application rate, together with an increase in the community similarity. The redundancy analyses (RDA) explained 43% of the total variation in operational taxonomic units and identified Na, pH, Cr and P as the main drivers of the archaeal community over time after application of highest CTS rates. CTS application changes the structure of Archaea community, with significant increase of Thaumarchaeota and Aenigmarchaeota groups, which can be further explored for its biotechnological use in contaminated soils.

LanguageEnglish
Article number7347
JournalScientific Reports
Volume9
DOIs
Publication statusPublished - 14 May 2019

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sludge
soil
microbial community
relative abundance
environmental factor
rate

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Miranda, A. R. L., Mendes, L. W., Lemos, L. N., Antunes, J. E. L., Amorim, M. R., Melo, V. M. M., ... Araujo, A. S. F. (2019). Dynamics of archaeal community in soil with application of composted tannery sludge. Scientific Reports, 9, [7347]. https://doi.org/10.1038/s41598-019-43478-y
Miranda, Ana Roberta Lima ; Mendes, Lucas William ; Lemos, Leandro Nascimento ; Antunes, Jadson Emanuel Lopes ; Amorim, Marineide Rodrigues ; Melo, Vania Maria Maciel ; de Melo, Wanderley Jose ; van den Brink, Paul J. ; Araujo, Ademir Sergio Ferreira. / Dynamics of archaeal community in soil with application of composted tannery sludge. In: Scientific Reports. 2019 ; Vol. 9.
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title = "Dynamics of archaeal community in soil with application of composted tannery sludge",
abstract = "Application of composted tannery sludge (CTS) could promote a shift in the structure of soil microbial communities. Although the effect of CTS on bacterial community has been studied, it is unclear how the composition and diversity of archaeal community respond to CTS amendment and which environmental factors drive the community over time. Here, we hypothesize that the Archaea structure and composition respond to CTS amendment over the time. CTS had been previously applied annually along 6 years and this assessment occurred for 180 days following the application in the 7 th year by using different rates (0, 2.5, 5, 10 and 20 ton ha −1 ). We used amplicon 16S rRNA sequencing to assess the changes in the structure of the archaeal community. Thaumarchaeota and Euryarchaeota were the most abundant phyla found in soils with application of CTS, with Thaumarchaeota dominating the sequences in all samples with relative abundances of >98{\%}. We observed a decreasing trend on the archaeal diversity over the time with increasing CTS application rate, together with an increase in the community similarity. The redundancy analyses (RDA) explained 43{\%} of the total variation in operational taxonomic units and identified Na, pH, Cr and P as the main drivers of the archaeal community over time after application of highest CTS rates. CTS application changes the structure of Archaea community, with significant increase of Thaumarchaeota and Aenigmarchaeota groups, which can be further explored for its biotechnological use in contaminated soils.",
author = "Miranda, {Ana Roberta Lima} and Mendes, {Lucas William} and Lemos, {Leandro Nascimento} and Antunes, {Jadson Emanuel Lopes} and Amorim, {Marineide Rodrigues} and Melo, {Vania Maria Maciel} and {de Melo}, {Wanderley Jose} and {van den Brink}, {Paul J.} and Araujo, {Ademir Sergio Ferreira}",
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Miranda, ARL, Mendes, LW, Lemos, LN, Antunes, JEL, Amorim, MR, Melo, VMM, de Melo, WJ, van den Brink, PJ & Araujo, ASF 2019, 'Dynamics of archaeal community in soil with application of composted tannery sludge', Scientific Reports, vol. 9, 7347. https://doi.org/10.1038/s41598-019-43478-y

Dynamics of archaeal community in soil with application of composted tannery sludge. / Miranda, Ana Roberta Lima; Mendes, Lucas William; Lemos, Leandro Nascimento; Antunes, Jadson Emanuel Lopes; Amorim, Marineide Rodrigues; Melo, Vania Maria Maciel; de Melo, Wanderley Jose; van den Brink, Paul J.; Araujo, Ademir Sergio Ferreira.

In: Scientific Reports, Vol. 9, 7347, 14.05.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Dynamics of archaeal community in soil with application of composted tannery sludge

AU - Miranda, Ana Roberta Lima

AU - Mendes, Lucas William

AU - Lemos, Leandro Nascimento

AU - Antunes, Jadson Emanuel Lopes

AU - Amorim, Marineide Rodrigues

AU - Melo, Vania Maria Maciel

AU - de Melo, Wanderley Jose

AU - van den Brink, Paul J.

AU - Araujo, Ademir Sergio Ferreira

PY - 2019/5/14

Y1 - 2019/5/14

N2 - Application of composted tannery sludge (CTS) could promote a shift in the structure of soil microbial communities. Although the effect of CTS on bacterial community has been studied, it is unclear how the composition and diversity of archaeal community respond to CTS amendment and which environmental factors drive the community over time. Here, we hypothesize that the Archaea structure and composition respond to CTS amendment over the time. CTS had been previously applied annually along 6 years and this assessment occurred for 180 days following the application in the 7 th year by using different rates (0, 2.5, 5, 10 and 20 ton ha −1 ). We used amplicon 16S rRNA sequencing to assess the changes in the structure of the archaeal community. Thaumarchaeota and Euryarchaeota were the most abundant phyla found in soils with application of CTS, with Thaumarchaeota dominating the sequences in all samples with relative abundances of >98%. We observed a decreasing trend on the archaeal diversity over the time with increasing CTS application rate, together with an increase in the community similarity. The redundancy analyses (RDA) explained 43% of the total variation in operational taxonomic units and identified Na, pH, Cr and P as the main drivers of the archaeal community over time after application of highest CTS rates. CTS application changes the structure of Archaea community, with significant increase of Thaumarchaeota and Aenigmarchaeota groups, which can be further explored for its biotechnological use in contaminated soils.

AB - Application of composted tannery sludge (CTS) could promote a shift in the structure of soil microbial communities. Although the effect of CTS on bacterial community has been studied, it is unclear how the composition and diversity of archaeal community respond to CTS amendment and which environmental factors drive the community over time. Here, we hypothesize that the Archaea structure and composition respond to CTS amendment over the time. CTS had been previously applied annually along 6 years and this assessment occurred for 180 days following the application in the 7 th year by using different rates (0, 2.5, 5, 10 and 20 ton ha −1 ). We used amplicon 16S rRNA sequencing to assess the changes in the structure of the archaeal community. Thaumarchaeota and Euryarchaeota were the most abundant phyla found in soils with application of CTS, with Thaumarchaeota dominating the sequences in all samples with relative abundances of >98%. We observed a decreasing trend on the archaeal diversity over the time with increasing CTS application rate, together with an increase in the community similarity. The redundancy analyses (RDA) explained 43% of the total variation in operational taxonomic units and identified Na, pH, Cr and P as the main drivers of the archaeal community over time after application of highest CTS rates. CTS application changes the structure of Archaea community, with significant increase of Thaumarchaeota and Aenigmarchaeota groups, which can be further explored for its biotechnological use in contaminated soils.

U2 - 10.1038/s41598-019-43478-y

DO - 10.1038/s41598-019-43478-y

M3 - Article

VL - 9

JO - Scientific Reports

T2 - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 7347

ER -

Miranda ARL, Mendes LW, Lemos LN, Antunes JEL, Amorim MR, Melo VMM et al. Dynamics of archaeal community in soil with application of composted tannery sludge. Scientific Reports. 2019 May 14;9. 7347. https://doi.org/10.1038/s41598-019-43478-y