Dynamic modelling of limitations on improving leaf CO2 assimilation under fluctuating irradiance

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Abstract

A dynamic model of leaf CO2 assimilation was developed as an extension of the canonical steady-state model, by adding the effects of energy-dependent non-photochemical quenching (qE), chloroplast movement, photoinhibition, regulation of enzyme activity in the Calvin cycle, metabolite concentrations, and dynamic CO2 diffusion. The model was calibrated and tested successfully using published measurements of gas exchange and chlorophyll fluorescence on Arabidopsis thaliana ecotype Col-0 and several photosynthetic mutants and transformants affecting the regulation of Rubisco activity (rca-2 and rwt43), non-photochemical quenching (npq4-1 and npq1-2), and sucrose synthesis (spsa1). The potential improvements on CO2 assimilation under fluctuating irradiance that can be achieved by removing the kinetic limitations on the regulation of enzyme activities, electron transport, and stomatal conductance were calculated in silico for different scenarios. The model predicted that the rates of activation of enzymes in the Calvin cycle and stomatal opening were the most limiting (up to 17% improvement) and that effects varied with the frequency of fluctuations. On the other hand, relaxation of qE and chloroplast movement had a strong effect on average low-irradiance CO2 assimilation (up to 10% improvement). Strong synergies among processes were found, such that removing all kinetic limitations simultaneously resulted in improvements of up to 32%.
LanguageEnglish
Pages589-604
JournalPlant Cell and Environment
Volume41
Issue number3
Early online date2018
DOIs
Publication statusPublished - 9 Feb 2018

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Photosynthesis
Chloroplasts
assimilation (physiology)
Calvin cycle
Ecotype
Ribulose-Bisphosphate Carboxylase
Enzyme Activation
Enzymes
Chlorophyll
chloroplasts
Electron Transport
Arabidopsis
Computer Simulation
enzyme activity
Sucrose
kinetics
leaves
enzyme activation
Fluorescence
Gases

Keywords

  • Arabidopsis
  • Lightflecks
  • Photosynthesis
  • Rubisco
  • Rubisco activase
  • Stomatal conductance
  • Sunflecks

Cite this

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title = "Dynamic modelling of limitations on improving leaf CO2 assimilation under fluctuating irradiance",
abstract = "A dynamic model of leaf CO2 assimilation was developed as an extension of the canonical steady-state model, by adding the effects of energy-dependent non-photochemical quenching (qE), chloroplast movement, photoinhibition, regulation of enzyme activity in the Calvin cycle, metabolite concentrations, and dynamic CO2 diffusion. The model was calibrated and tested successfully using published measurements of gas exchange and chlorophyll fluorescence on Arabidopsis thaliana ecotype Col-0 and several photosynthetic mutants and transformants affecting the regulation of Rubisco activity (rca-2 and rwt43), non-photochemical quenching (npq4-1 and npq1-2), and sucrose synthesis (spsa1). The potential improvements on CO2 assimilation under fluctuating irradiance that can be achieved by removing the kinetic limitations on the regulation of enzyme activities, electron transport, and stomatal conductance were calculated in silico for different scenarios. The model predicted that the rates of activation of enzymes in the Calvin cycle and stomatal opening were the most limiting (up to 17{\%} improvement) and that effects varied with the frequency of fluctuations. On the other hand, relaxation of qE and chloroplast movement had a strong effect on average low-irradiance CO2 assimilation (up to 10{\%} improvement). Strong synergies among processes were found, such that removing all kinetic limitations simultaneously resulted in improvements of up to 32{\%}.",
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author = "Alejandro Morales and Elias Kaiser and Xinyou Yin and Jeremy Harbinson and Jaap Molenaar and Driever, {Steven M.} and Struik, {Paul C.}",
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T1 - Dynamic modelling of limitations on improving leaf CO2 assimilation under fluctuating irradiance

AU - Morales, Alejandro

AU - Kaiser, Elias

AU - Yin, Xinyou

AU - Harbinson, Jeremy

AU - Molenaar, Jaap

AU - Driever, Steven M.

AU - Struik, Paul C.

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N2 - A dynamic model of leaf CO2 assimilation was developed as an extension of the canonical steady-state model, by adding the effects of energy-dependent non-photochemical quenching (qE), chloroplast movement, photoinhibition, regulation of enzyme activity in the Calvin cycle, metabolite concentrations, and dynamic CO2 diffusion. The model was calibrated and tested successfully using published measurements of gas exchange and chlorophyll fluorescence on Arabidopsis thaliana ecotype Col-0 and several photosynthetic mutants and transformants affecting the regulation of Rubisco activity (rca-2 and rwt43), non-photochemical quenching (npq4-1 and npq1-2), and sucrose synthesis (spsa1). The potential improvements on CO2 assimilation under fluctuating irradiance that can be achieved by removing the kinetic limitations on the regulation of enzyme activities, electron transport, and stomatal conductance were calculated in silico for different scenarios. The model predicted that the rates of activation of enzymes in the Calvin cycle and stomatal opening were the most limiting (up to 17% improvement) and that effects varied with the frequency of fluctuations. On the other hand, relaxation of qE and chloroplast movement had a strong effect on average low-irradiance CO2 assimilation (up to 10% improvement). Strong synergies among processes were found, such that removing all kinetic limitations simultaneously resulted in improvements of up to 32%.

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KW - Arabidopsis

KW - Lightflecks

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KW - Rubisco activase

KW - Stomatal conductance

KW - Sunflecks

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