The relative importance of duration of sward regrowth and fill and fermentation in the rumen on the control of grazing time and intake rate during the first grazing session of the day was studied. Four lactating dairy cows were allowed to graze ryegrass (Lolium perenne) swards, with five different regrowth periods after mowing (6, 9, 16, 22, and 30 d). The cows were allowed to graze until they stopped voluntarily (cessation of grazing activity for at least 15 min). Before and after grazing the rumen contents were evacuated, weighed, sampled, and returned to the animals. Samples of rumen liquid were taken immediately before rumen evacuation and approximately 30, 60, 120, and 240 min after the grazing session was finished. Grazing time and intake rate did not follow a significant trend with period of regrowth. Bite rate did not change significantly with duration of regrowth with cows exhibiting high rates of biting for all the sward conditions. Rumen pools sizes of dry matter, neutral detergent fiber, and volatile fatty acids (VFA), measured after grazing, increased significantly with days of sward regrowth, even though the changes over days were small. Concentration of VFA followed a significant quadratic trend with a maximum concentration observed at approximately 110 min after cessation of grazing. In this study, rumen fill, VFA (either total or major components), ammonia, pH, and osmotic pressure as individual variables were not correlated with grazing time or dry matter intake.