Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger

F. Mela, K. Fritsche, W. de Boer, J.A. van Veen, L.H. de Graaff, M. van den Berg, J.H.J. Leveau

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60 Citations (Scopus)

Abstract

Interactions between bacteria and fungi cover a wide range of incentives, mechanisms and outcomes. The genus Collimonas consists of soil bacteria that are known for their antifungal activity and ability to grow at the expense of living fungi. In non-contact confrontation assays with the fungus Aspergillus niger, Collimonas fungivorans showed accumulation of biomass concomitant with inhibition of hyphal spread. Through microarray analysis of bacterial and fungal mRNA from the confrontation arena, we gained new insights into the mechanisms underlying the fungistatic effect and mycophagous phenotype of collimonads. Collimonas responded to the fungus by activating genes for the utilization of fungal-derived compounds and for production of a putative antifungal compound. In A. niger, differentially expressed genes included those involved in lipid and cell wall metabolism and cell defense, which correlated well with the hyphal deformations that were observed microscopically. Transcriptional profiles revealed distress in both partners: downregulation of ribosomal proteins and upregulation of mobile genetic elements in the bacteria and expression of endoplasmic reticulum stress and conidia-related genes in the fungus. Both partners experienced nitrogen shortage in each other's presence. Overall, our results indicate that the Collimonas/Aspergillus interaction is a complex interplay between trophism, antibiosis and competition for nutrients
Original languageEnglish
Pages (from-to)1494-1504
JournalISME Journal
Volume5
Issue number9
DOIs
Publication statusPublished - 2011

Keywords

  • probe level data
  • gene-expression
  • corynebacterium-glutamicum
  • bacillus-subtilis
  • ribosomal-proteins
  • neurospora-crassa
  • fungal hyphae
  • fusaric acid
  • citric-acid
  • growth

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  • Datasets

    Listening in on a bacterial/fungal dialog: transcriptional profiling of an antagonistic interaction

    Mela, F. (Creator), Leveau, J. H. J. (Creator), van Veen, J. A. (Creator), Fritsche, K. (Creator), de Graaff, L. H. (Creator), van den Berg, M. (Creator), de Boer, W. (Creator), Wageningen UR, 3 May 2014

    Dataset

    Cite this

    Mela, F., Fritsche, K., de Boer, W., van Veen, J. A., de Graaff, L. H., van den Berg, M., & Leveau, J. H. J. (2011). Dual transcriptional profiling of a bacterial/fungal confrontation: Collimonas fungivorans versus Aspergillus niger. ISME Journal, 5(9), 1494-1504. https://doi.org/10.1038/ismej.2011.29