Dual biosensor immunoassay-directed identification of fluoroquinolones in chicken muscle by liquid chromatography electrospray time-of-flight mass spectrometry

G.R. Marchesini, W. Haasnoot, P. Delahaut, H. Gercek, M.W.F. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

41 Citations (Scopus)

Abstract

Fluoroquinolones (FQs) are synthetic antibiotics of broad-spectrum antibacterial activity widely used to treat infections in farmed fish, turkeys, pigs, calves and poultry. Monitoring these substances residues is therefore regulated by law. For the detection of FQs, we studied the feasibility of coupling the simultaneous screening of several FQs, using a dual surface plasmon resonance (SPR) biosensor immunoassay (BIA), in parallel, with an analytical chemical methodology for their identification. Six FQs were simultaneously screened at or below their maximum residue level (MRL) in chicken muscle using a multi-FQ BIA for norfloxacin, ciprofloxacin, enrofloxacin, difloxacin and sarafloxacin, and a specific BIA for flumequine. The two BIAs were serially coupled in a multi-channel SPR biosensor featuring a dual BIA in a competitive inhibition format. The samples non-compliant during the screening with the dual BIA were further concentrated and fractionated with gradient liquid chromatography (LC). The effluent was splitted toward two 96-well fraction collectors resulting in two identical 96-well plates. One was re-screened with the dual BIA to identify the immunoactive fractions and direct the identification efforts toward the relevant fractions in the second well-plate with high resolution LC-electrospray time-of-flight mass spectrometry (ESI-TOFMS). The system not only allows the possibility to screen and identify known FQs, but also to discover unknown chemicals of similar structure which show activity in the dual BIA.
LanguageEnglish
Pages259-268
JournalAnalytica Chimica Acta
Volume586
Issue number1-2
DOIs
Publication statusPublished - 2007

Fingerprint

immunoassay
Fluoroquinolones
Liquid chromatography
Biosensing Techniques
Immunoassay
Biosensors
Liquid Chromatography
Mass spectrometry
Muscle
liquid chromatography
Chickens
Mass Spectrometry
muscle
mass spectrometry
Muscles
Surface Plasmon Resonance
flumequine
Surface plasmon resonance
difloxacin
sarafloxacin

Keywords

  • residues
  • quinolones
  • food
  • antibiotics
  • antibodies
  • ofloxacin
  • products
  • tissues
  • urine
  • elisa

Cite this

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Dual biosensor immunoassay-directed identification of fluoroquinolones in chicken muscle by liquid chromatography electrospray time-of-flight mass spectrometry. / Marchesini, G.R.; Haasnoot, W.; Delahaut, P.; Gercek, H.; Nielen, M.W.F.

In: Analytica Chimica Acta, Vol. 586, No. 1-2, 2007, p. 259-268.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Dual biosensor immunoassay-directed identification of fluoroquinolones in chicken muscle by liquid chromatography electrospray time-of-flight mass spectrometry

AU - Marchesini, G.R.

AU - Haasnoot, W.

AU - Delahaut, P.

AU - Gercek, H.

AU - Nielen, M.W.F.

PY - 2007

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KW - residues

KW - quinolones

KW - food

KW - antibiotics

KW - antibodies

KW - ofloxacin

KW - products

KW - tissues

KW - urine

KW - elisa

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DO - 10.1016/j.aca.2006.11.013

M3 - Article

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SP - 259

EP - 268

JO - Analytica Chimica Acta

T2 - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

IS - 1-2

ER -