Draft genome sequence of the nitrogen-fixing Rhizobium sullae type strain IS123T focusing on the key genes for symbiosis with its host Hedysarum coronarium L.

Gaurav Sablok*, Riccardo Rosselli, Torsten Seeman, Robin van Velzen, Elisa Polone, Alessio Giacomini, Nicola La Porta, Rene Geurts, Rosella Muresu, Andrea Squartini

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

The prominent feature of rhizobia is their molecular dialogue with plant hosts. Such interaction is enabled by the presence of a series of symbiotic genes encoding for the synthesis and export of signals triggering organogenetic and physiological responses in the plant. The genome of the Rhizobium sullae type strain IS123T nodulating the legume Hedysarum coronarium, was sequenced and resulted in 317 scaffolds for a total assembled size of 7,889,576 bp. Its features were compared with those of genomes from rhizobia representing an increasing gradient of taxonomical distance, from a conspecific isolate (Rhizobium sullae WSM1592), to two congeneric cases (Rhizobium leguminosarum bv. viciae and Rhizobium etli) and up to different genera within the legume-nodulating taxa. The host plant is of agricultural importance, but, unlike the majority of other domesticated plant species, it is able to survive quite well in the wild. Data showed that that the type strain of R. sullae, isolated from a wild host specimen, is endowed with a richer array of symbiotic genes in comparison to other strains, species or genera of rhizobia that were rescued from domesticated plant ecotypes. The analysis revealed that the bacterium by itself is incapable of surviving in the extreme conditions that its host plant can tolerate. When exposed to drought or alkaline condition, the bacterium depends on its host to survive. Data are consistent with the view of the plant phenotype as the primary factor enabling symbiotic nitrogen fixing bacteria to survive in otherwise limiting environments.

Original languageEnglish
Article number1348
JournalFrontiers in Microbiology
Volume8
Issue numberJUL
DOIs
Publication statusPublished - 2017

Fingerprint

Rhizobium
Symbiosis
Nitrogen
Genome
Genes
Fabaceae
Rhizobium etli
Rhizobium leguminosarum
Ecotype
Bacteria
Droughts
Causality
Phenotype

Keywords

  • Hedysarum coronarium
  • Host-specific symbiotic adaptation
  • Nitrogen fixation
  • Nod genes
  • Rhizobium sullae
  • Root-nodule bacteria
  • Sulla coronaria
  • Type strain

Cite this

Sablok, Gaurav ; Rosselli, Riccardo ; Seeman, Torsten ; van Velzen, Robin ; Polone, Elisa ; Giacomini, Alessio ; La Porta, Nicola ; Geurts, Rene ; Muresu, Rosella ; Squartini, Andrea. / Draft genome sequence of the nitrogen-fixing Rhizobium sullae type strain IS123T focusing on the key genes for symbiosis with its host Hedysarum coronarium L. In: Frontiers in Microbiology. 2017 ; Vol. 8, No. JUL.
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title = "Draft genome sequence of the nitrogen-fixing Rhizobium sullae type strain IS123T focusing on the key genes for symbiosis with its host Hedysarum coronarium L.",
abstract = "The prominent feature of rhizobia is their molecular dialogue with plant hosts. Such interaction is enabled by the presence of a series of symbiotic genes encoding for the synthesis and export of signals triggering organogenetic and physiological responses in the plant. The genome of the Rhizobium sullae type strain IS123T nodulating the legume Hedysarum coronarium, was sequenced and resulted in 317 scaffolds for a total assembled size of 7,889,576 bp. Its features were compared with those of genomes from rhizobia representing an increasing gradient of taxonomical distance, from a conspecific isolate (Rhizobium sullae WSM1592), to two congeneric cases (Rhizobium leguminosarum bv. viciae and Rhizobium etli) and up to different genera within the legume-nodulating taxa. The host plant is of agricultural importance, but, unlike the majority of other domesticated plant species, it is able to survive quite well in the wild. Data showed that that the type strain of R. sullae, isolated from a wild host specimen, is endowed with a richer array of symbiotic genes in comparison to other strains, species or genera of rhizobia that were rescued from domesticated plant ecotypes. The analysis revealed that the bacterium by itself is incapable of surviving in the extreme conditions that its host plant can tolerate. When exposed to drought or alkaline condition, the bacterium depends on its host to survive. Data are consistent with the view of the plant phenotype as the primary factor enabling symbiotic nitrogen fixing bacteria to survive in otherwise limiting environments.",
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author = "Gaurav Sablok and Riccardo Rosselli and Torsten Seeman and {van Velzen}, Robin and Elisa Polone and Alessio Giacomini and {La Porta}, Nicola and Rene Geurts and Rosella Muresu and Andrea Squartini",
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doi = "10.3389/fmicb.2017.01348",
language = "English",
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Draft genome sequence of the nitrogen-fixing Rhizobium sullae type strain IS123T focusing on the key genes for symbiosis with its host Hedysarum coronarium L. / Sablok, Gaurav; Rosselli, Riccardo; Seeman, Torsten; van Velzen, Robin; Polone, Elisa; Giacomini, Alessio; La Porta, Nicola; Geurts, Rene; Muresu, Rosella; Squartini, Andrea.

In: Frontiers in Microbiology, Vol. 8, No. JUL, 1348, 2017.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Sablok, Gaurav

AU - Rosselli, Riccardo

AU - Seeman, Torsten

AU - van Velzen, Robin

AU - Polone, Elisa

AU - Giacomini, Alessio

AU - La Porta, Nicola

AU - Geurts, Rene

AU - Muresu, Rosella

AU - Squartini, Andrea

PY - 2017

Y1 - 2017

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AB - The prominent feature of rhizobia is their molecular dialogue with plant hosts. Such interaction is enabled by the presence of a series of symbiotic genes encoding for the synthesis and export of signals triggering organogenetic and physiological responses in the plant. The genome of the Rhizobium sullae type strain IS123T nodulating the legume Hedysarum coronarium, was sequenced and resulted in 317 scaffolds for a total assembled size of 7,889,576 bp. Its features were compared with those of genomes from rhizobia representing an increasing gradient of taxonomical distance, from a conspecific isolate (Rhizobium sullae WSM1592), to two congeneric cases (Rhizobium leguminosarum bv. viciae and Rhizobium etli) and up to different genera within the legume-nodulating taxa. The host plant is of agricultural importance, but, unlike the majority of other domesticated plant species, it is able to survive quite well in the wild. Data showed that that the type strain of R. sullae, isolated from a wild host specimen, is endowed with a richer array of symbiotic genes in comparison to other strains, species or genera of rhizobia that were rescued from domesticated plant ecotypes. The analysis revealed that the bacterium by itself is incapable of surviving in the extreme conditions that its host plant can tolerate. When exposed to drought or alkaline condition, the bacterium depends on its host to survive. Data are consistent with the view of the plant phenotype as the primary factor enabling symbiotic nitrogen fixing bacteria to survive in otherwise limiting environments.

KW - Hedysarum coronarium

KW - Host-specific symbiotic adaptation

KW - Nitrogen fixation

KW - Nod genes

KW - Rhizobium sullae

KW - Root-nodule bacteria

KW - Sulla coronaria

KW - Type strain

U2 - 10.3389/fmicb.2017.01348

DO - 10.3389/fmicb.2017.01348

M3 - Article

VL - 8

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

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