Abstract
The prominent feature of rhizobia is their molecular dialogue with plant hosts. Such interaction is enabled by the presence of a series of symbiotic genes encoding for the synthesis and export of signals triggering organogenetic and physiological responses in the plant. The genome of the Rhizobium sullae type strain IS123T nodulating the legume Hedysarum coronarium, was sequenced and resulted in 317 scaffolds for a total assembled size of 7,889,576 bp. Its features were compared with those of genomes from rhizobia representing an increasing gradient of taxonomical distance, from a conspecific isolate (Rhizobium sullae WSM1592), to two congeneric cases (Rhizobium leguminosarum bv. viciae and Rhizobium etli) and up to different genera within the legume-nodulating taxa. The host plant is of agricultural importance, but, unlike the majority of other domesticated plant species, it is able to survive quite well in the wild. Data showed that that the type strain of R. sullae, isolated from a wild host specimen, is endowed with a richer array of symbiotic genes in comparison to other strains, species or genera of rhizobia that were rescued from domesticated plant ecotypes. The analysis revealed that the bacterium by itself is incapable of surviving in the extreme conditions that its host plant can tolerate. When exposed to drought or alkaline condition, the bacterium depends on its host to survive. Data are consistent with the view of the plant phenotype as the primary factor enabling symbiotic nitrogen fixing bacteria to survive in otherwise limiting environments.
| Original language | English |
|---|---|
| Article number | 1348 |
| Journal | Frontiers in Microbiology |
| Volume | 8 |
| Issue number | JUL |
| DOIs | |
| Publication status | Published - 2017 |
Fingerprint
Keywords
- Hedysarum coronarium
- Host-specific symbiotic adaptation
- Nitrogen fixation
- Nod genes
- Rhizobium sullae
- Root-nodule bacteria
- Sulla coronaria
- Type strain
Cite this
}
Draft genome sequence of the nitrogen-fixing Rhizobium sullae type strain IS123T focusing on the key genes for symbiosis with its host Hedysarum coronarium L. / Sablok, Gaurav; Rosselli, Riccardo; Seeman, Torsten; van Velzen, Robin; Polone, Elisa; Giacomini, Alessio; La Porta, Nicola; Geurts, Rene; Muresu, Rosella; Squartini, Andrea.
In: Frontiers in Microbiology, Vol. 8, No. JUL, 1348, 2017.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Draft genome sequence of the nitrogen-fixing Rhizobium sullae type strain IS123T focusing on the key genes for symbiosis with its host Hedysarum coronarium L.
AU - Sablok, Gaurav
AU - Rosselli, Riccardo
AU - Seeman, Torsten
AU - van Velzen, Robin
AU - Polone, Elisa
AU - Giacomini, Alessio
AU - La Porta, Nicola
AU - Geurts, Rene
AU - Muresu, Rosella
AU - Squartini, Andrea
PY - 2017
Y1 - 2017
N2 - The prominent feature of rhizobia is their molecular dialogue with plant hosts. Such interaction is enabled by the presence of a series of symbiotic genes encoding for the synthesis and export of signals triggering organogenetic and physiological responses in the plant. The genome of the Rhizobium sullae type strain IS123T nodulating the legume Hedysarum coronarium, was sequenced and resulted in 317 scaffolds for a total assembled size of 7,889,576 bp. Its features were compared with those of genomes from rhizobia representing an increasing gradient of taxonomical distance, from a conspecific isolate (Rhizobium sullae WSM1592), to two congeneric cases (Rhizobium leguminosarum bv. viciae and Rhizobium etli) and up to different genera within the legume-nodulating taxa. The host plant is of agricultural importance, but, unlike the majority of other domesticated plant species, it is able to survive quite well in the wild. Data showed that that the type strain of R. sullae, isolated from a wild host specimen, is endowed with a richer array of symbiotic genes in comparison to other strains, species or genera of rhizobia that were rescued from domesticated plant ecotypes. The analysis revealed that the bacterium by itself is incapable of surviving in the extreme conditions that its host plant can tolerate. When exposed to drought or alkaline condition, the bacterium depends on its host to survive. Data are consistent with the view of the plant phenotype as the primary factor enabling symbiotic nitrogen fixing bacteria to survive in otherwise limiting environments.
AB - The prominent feature of rhizobia is their molecular dialogue with plant hosts. Such interaction is enabled by the presence of a series of symbiotic genes encoding for the synthesis and export of signals triggering organogenetic and physiological responses in the plant. The genome of the Rhizobium sullae type strain IS123T nodulating the legume Hedysarum coronarium, was sequenced and resulted in 317 scaffolds for a total assembled size of 7,889,576 bp. Its features were compared with those of genomes from rhizobia representing an increasing gradient of taxonomical distance, from a conspecific isolate (Rhizobium sullae WSM1592), to two congeneric cases (Rhizobium leguminosarum bv. viciae and Rhizobium etli) and up to different genera within the legume-nodulating taxa. The host plant is of agricultural importance, but, unlike the majority of other domesticated plant species, it is able to survive quite well in the wild. Data showed that that the type strain of R. sullae, isolated from a wild host specimen, is endowed with a richer array of symbiotic genes in comparison to other strains, species or genera of rhizobia that were rescued from domesticated plant ecotypes. The analysis revealed that the bacterium by itself is incapable of surviving in the extreme conditions that its host plant can tolerate. When exposed to drought or alkaline condition, the bacterium depends on its host to survive. Data are consistent with the view of the plant phenotype as the primary factor enabling symbiotic nitrogen fixing bacteria to survive in otherwise limiting environments.
KW - Hedysarum coronarium
KW - Host-specific symbiotic adaptation
KW - Nitrogen fixation
KW - Nod genes
KW - Rhizobium sullae
KW - Root-nodule bacteria
KW - Sulla coronaria
KW - Type strain
U2 - 10.3389/fmicb.2017.01348
DO - 10.3389/fmicb.2017.01348
M3 - Article
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
SN - 1664-302X
IS - JUL
M1 - 1348
ER -