Double kill : the DNase and RNase activity of CRISPR-Cas type III systems

Yifan Zhu

doi:10.18174/471572

Double kill : the DNase and RNase activity of CRISPR-Cas type III systems

Yifan Zhu

Research output: Thesis › internal PhD, WU

Abstract

Since the discovery of CRISPR-Cas immune systems astonishing progress has been made on revealing their mechanistic foundations. Due to the immense potential as genome engineering tools, research has mainly focused on a subset of Cas nucleases that target DNA. In addition, however, distinct types of RNA-targeting CRISPR-Cas systems have been identified. The focus of my PhD thesis is on the interference and recognition mechanisms of the RNA targeting type III systems, their biological relevance and their potential for applications. The DNA substrate cleavage mechanism is studied and discussed as well.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	Wageningen University
Supervisors/Advisors	de Vos, Willem, Promotor van der Oost, John, Promotor Staals, Raymond, Co-promotor
Award date	3 May 2019
Place of Publication	Wageningen
Publisher	Wageningen University
Print ISBNs	9789463434362
DOIs	https://doi.org/10.18174/471572
Publication status	Published - 2019

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1 Finished

Microbial Host-virus interaction in human GI tract
Zhu, Y., Staals, R., de Vos, W. & van der Oost, J.
1/04/14 → 3/05/19
Project: PhD

Cite this

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title = "Double kill : the DNase and RNase activity of CRISPR-Cas type III systems",

abstract = "Since the discovery of CRISPR-Cas immune systems astonishing progress has been made on revealing their mechanistic foundations. Due to the immense potential as genome engineering tools, research has mainly focused on a subset of Cas nucleases that target DNA. In addition, however, distinct types of RNA-targeting CRISPR-Cas systems have been identified. The focus of my PhD thesis is on the interference and recognition mechanisms of the RNA targeting type III systems, their biological relevance and their potential for applications. The DNA substrate cleavage mechanism is studied and discussed as well.",

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N2 - Since the discovery of CRISPR-Cas immune systems astonishing progress has been made on revealing their mechanistic foundations. Due to the immense potential as genome engineering tools, research has mainly focused on a subset of Cas nucleases that target DNA. In addition, however, distinct types of RNA-targeting CRISPR-Cas systems have been identified. The focus of my PhD thesis is on the interference and recognition mechanisms of the RNA targeting type III systems, their biological relevance and their potential for applications. The DNA substrate cleavage mechanism is studied and discussed as well.

AB - Since the discovery of CRISPR-Cas immune systems astonishing progress has been made on revealing their mechanistic foundations. Due to the immense potential as genome engineering tools, research has mainly focused on a subset of Cas nucleases that target DNA. In addition, however, distinct types of RNA-targeting CRISPR-Cas systems have been identified. The focus of my PhD thesis is on the interference and recognition mechanisms of the RNA targeting type III systems, their biological relevance and their potential for applications. The DNA substrate cleavage mechanism is studied and discussed as well.

UR - https://edepot.wur.nl/471572

U2 - 10.18174/471572

DO - 10.18174/471572

M3 - internal PhD, WU

SN - 9789463434362

PB - Wageningen University

CY - Wageningen

ER -

Double kill : the DNase and RNase activity of CRISPR-Cas type III systems

Abstract

Access to Document

Microbial Host-virus interaction in human GI tract

Cite this