Double kill : the DNase and RNase activity of CRISPR-Cas type III systems

Yifan Zhu

Research output: Thesisinternal PhD, WU

Abstract

Since the discovery of CRISPR-Cas immune systems astonishing progress has been made on revealing their mechanistic foundations. Due to the immense potential as genome engineering tools, research has mainly focused on a subset of Cas nucleases that target DNA. In addition, however, distinct types of RNA-targeting CRISPR-Cas systems have been identified. The focus of my PhD thesis is on the interference and recognition mechanisms of the RNA targeting type III systems, their biological relevance and their potential for applications. The DNA substrate cleavage mechanism is studied and discussed as well.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • de Vos, Willem, Promotor
  • van der Oost, John, Promotor
  • Staals, Raymond, Co-promotor
Award date3 May 2019
Place of PublicationWageningen
Publisher
Print ISBNs9789463434362
DOIs
Publication statusPublished - 2019

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