Dothideomycete Plant Pathogens Require Specific Virulence Factors for Colonization and Host Plants Have Developed Specific R Genes for Defence

P.J.G.M. de Wit, C.H. Mesarich, B. Ökmen, A. van der Burgt, Y. Iida, E. Battaglia, H.G. Beenen, S.A. Griffiths, J.A.R. Collemare, R.E. Bradshaw

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

The tomato pathogen Cladosporium fulvum is a Dothideomycete that is most related to the hemi-biotrophic fungal pine pathogen Dothistroma septosporum. We study genomic adaptations in these and related fungi that can explain host plant-specificity. The C. fulvum genomic sequence allowed us to identify and study its complete effector catalogue including three core effectors (Avr4, Ecp2 and Ecp6). The genome of D. septosporum harbors the highest number of homologs of C. fulvum effectors, but some are pseudogenized. Both C. fulvum and D. septosporum contain a large number of genes involved in the production of secondary metabolites. C. fulvum seems to adapt its host plant tomato by down-regulation or pseudogenization of genes involved in the production of toxic secondary metabolites. One example is dothiostromin, a toxin produced by D. septosporum during colonization of pine needle. C. fulvum contains all genes required for the production of this toxin, but a few of these genes are pseudogenized. Apart from new effectors (Avr5) we have also identified new pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) from C. fulvum. Both represent cell-wall degrading enzymes produced by C. fulvum during infection of tomato. We also found that C. fulvum has adapted to grow on tomato by secreting the enzyme a-tomatinase that hydrolyses the antifungal tomato saponin a-tomatine into the non-toxic compounds lycotetraose and tomatidine.
Original languageEnglish
Title of host publicationBook of Abstracts Joint Meeting American Phytopathological Society and Canadian Phytopathological Society
Pages44-S
Publication statusPublished - 2014
Event2014 APS-CPS Joint Meeting, Minneapolis, Minnesota, USA -
Duration: 9 Aug 201413 Aug 2014

Conference

Conference2014 APS-CPS Joint Meeting, Minneapolis, Minnesota, USA
Period9/08/1413/08/14

Fingerprint

Dothideomycetes
Passalora fulva
plant pathogens
virulence
host plants
tomatoes
genes
secondary metabolites
pathogens
toxins
Dothistroma
genomics
conifer needles
enzymes
saponins
Pinus
hydrolysis
cell walls

Cite this

de Wit, P. J. G. M., Mesarich, C. H., Ökmen, B., van der Burgt, A., Iida, Y., Battaglia, E., ... Bradshaw, R. E. (2014). Dothideomycete Plant Pathogens Require Specific Virulence Factors for Colonization and Host Plants Have Developed Specific R Genes for Defence. In Book of Abstracts Joint Meeting American Phytopathological Society and Canadian Phytopathological Society (pp. 44-S)
de Wit, P.J.G.M. ; Mesarich, C.H. ; Ökmen, B. ; van der Burgt, A. ; Iida, Y. ; Battaglia, E. ; Beenen, H.G. ; Griffiths, S.A. ; Collemare, J.A.R. ; Bradshaw, R.E. / Dothideomycete Plant Pathogens Require Specific Virulence Factors for Colonization and Host Plants Have Developed Specific R Genes for Defence. Book of Abstracts Joint Meeting American Phytopathological Society and Canadian Phytopathological Society. 2014. pp. 44-S
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title = "Dothideomycete Plant Pathogens Require Specific Virulence Factors for Colonization and Host Plants Have Developed Specific R Genes for Defence",
abstract = "The tomato pathogen Cladosporium fulvum is a Dothideomycete that is most related to the hemi-biotrophic fungal pine pathogen Dothistroma septosporum. We study genomic adaptations in these and related fungi that can explain host plant-specificity. The C. fulvum genomic sequence allowed us to identify and study its complete effector catalogue including three core effectors (Avr4, Ecp2 and Ecp6). The genome of D. septosporum harbors the highest number of homologs of C. fulvum effectors, but some are pseudogenized. Both C. fulvum and D. septosporum contain a large number of genes involved in the production of secondary metabolites. C. fulvum seems to adapt its host plant tomato by down-regulation or pseudogenization of genes involved in the production of toxic secondary metabolites. One example is dothiostromin, a toxin produced by D. septosporum during colonization of pine needle. C. fulvum contains all genes required for the production of this toxin, but a few of these genes are pseudogenized. Apart from new effectors (Avr5) we have also identified new pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) from C. fulvum. Both represent cell-wall degrading enzymes produced by C. fulvum during infection of tomato. We also found that C. fulvum has adapted to grow on tomato by secreting the enzyme a-tomatinase that hydrolyses the antifungal tomato saponin a-tomatine into the non-toxic compounds lycotetraose and tomatidine.",
author = "{de Wit}, P.J.G.M. and C.H. Mesarich and B. {\"O}kmen and {van der Burgt}, A. and Y. Iida and E. Battaglia and H.G. Beenen and S.A. Griffiths and J.A.R. Collemare and R.E. Bradshaw",
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de Wit, PJGM, Mesarich, CH, Ökmen, B, van der Burgt, A, Iida, Y, Battaglia, E, Beenen, HG, Griffiths, SA, Collemare, JAR & Bradshaw, RE 2014, Dothideomycete Plant Pathogens Require Specific Virulence Factors for Colonization and Host Plants Have Developed Specific R Genes for Defence. in Book of Abstracts Joint Meeting American Phytopathological Society and Canadian Phytopathological Society. pp. 44-S, 2014 APS-CPS Joint Meeting, Minneapolis, Minnesota, USA, 9/08/14.

Dothideomycete Plant Pathogens Require Specific Virulence Factors for Colonization and Host Plants Have Developed Specific R Genes for Defence. / de Wit, P.J.G.M.; Mesarich, C.H.; Ökmen, B.; van der Burgt, A.; Iida, Y.; Battaglia, E.; Beenen, H.G.; Griffiths, S.A.; Collemare, J.A.R.; Bradshaw, R.E.

Book of Abstracts Joint Meeting American Phytopathological Society and Canadian Phytopathological Society. 2014. p. 44-S.

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

TY - CHAP

T1 - Dothideomycete Plant Pathogens Require Specific Virulence Factors for Colonization and Host Plants Have Developed Specific R Genes for Defence

AU - de Wit, P.J.G.M.

AU - Mesarich, C.H.

AU - Ökmen, B.

AU - van der Burgt, A.

AU - Iida, Y.

AU - Battaglia, E.

AU - Beenen, H.G.

AU - Griffiths, S.A.

AU - Collemare, J.A.R.

AU - Bradshaw, R.E.

PY - 2014

Y1 - 2014

N2 - The tomato pathogen Cladosporium fulvum is a Dothideomycete that is most related to the hemi-biotrophic fungal pine pathogen Dothistroma septosporum. We study genomic adaptations in these and related fungi that can explain host plant-specificity. The C. fulvum genomic sequence allowed us to identify and study its complete effector catalogue including three core effectors (Avr4, Ecp2 and Ecp6). The genome of D. septosporum harbors the highest number of homologs of C. fulvum effectors, but some are pseudogenized. Both C. fulvum and D. septosporum contain a large number of genes involved in the production of secondary metabolites. C. fulvum seems to adapt its host plant tomato by down-regulation or pseudogenization of genes involved in the production of toxic secondary metabolites. One example is dothiostromin, a toxin produced by D. septosporum during colonization of pine needle. C. fulvum contains all genes required for the production of this toxin, but a few of these genes are pseudogenized. Apart from new effectors (Avr5) we have also identified new pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) from C. fulvum. Both represent cell-wall degrading enzymes produced by C. fulvum during infection of tomato. We also found that C. fulvum has adapted to grow on tomato by secreting the enzyme a-tomatinase that hydrolyses the antifungal tomato saponin a-tomatine into the non-toxic compounds lycotetraose and tomatidine.

AB - The tomato pathogen Cladosporium fulvum is a Dothideomycete that is most related to the hemi-biotrophic fungal pine pathogen Dothistroma septosporum. We study genomic adaptations in these and related fungi that can explain host plant-specificity. The C. fulvum genomic sequence allowed us to identify and study its complete effector catalogue including three core effectors (Avr4, Ecp2 and Ecp6). The genome of D. septosporum harbors the highest number of homologs of C. fulvum effectors, but some are pseudogenized. Both C. fulvum and D. septosporum contain a large number of genes involved in the production of secondary metabolites. C. fulvum seems to adapt its host plant tomato by down-regulation or pseudogenization of genes involved in the production of toxic secondary metabolites. One example is dothiostromin, a toxin produced by D. septosporum during colonization of pine needle. C. fulvum contains all genes required for the production of this toxin, but a few of these genes are pseudogenized. Apart from new effectors (Avr5) we have also identified new pathogen-associated molecular patterns (PAMPs) and damage-associated molecular patterns (DAMPs) from C. fulvum. Both represent cell-wall degrading enzymes produced by C. fulvum during infection of tomato. We also found that C. fulvum has adapted to grow on tomato by secreting the enzyme a-tomatinase that hydrolyses the antifungal tomato saponin a-tomatine into the non-toxic compounds lycotetraose and tomatidine.

M3 - Abstract

SP - 44-S

BT - Book of Abstracts Joint Meeting American Phytopathological Society and Canadian Phytopathological Society

ER -

de Wit PJGM, Mesarich CH, Ökmen B, van der Burgt A, Iida Y, Battaglia E et al. Dothideomycete Plant Pathogens Require Specific Virulence Factors for Colonization and Host Plants Have Developed Specific R Genes for Defence. In Book of Abstracts Joint Meeting American Phytopathological Society and Canadian Phytopathological Society. 2014. p. 44-S