DORNRÖSCHEN is a direct target of the auxin response factor MONOPTEROS in the Arabidopsis embryo

M. Cole, J. Chandler, D. Weijers, B. Jacobs, P. Comelli, W. Werr

Research output: Contribution to journalArticleAcademicpeer-review

106 Citations (Scopus)

Abstract

DORNRÖSCHEN (DRN), which encodes a member of the AP2-type transcription factor family, contributes to auxin transport and perception in the Arabidopsis embryo. Live imaging performed with transcriptional or translational GFP fusions shows DRN to be activated in the apical cell after the first zygotic division, to act cell-autonomously and to be expressed in single cells extending laterally from the apical shoot stem-cell zone at the position of incipient leaf primordia. Here, we show that the Auxin response factor (ARF) MONOPTEROS (MP) directly controls DRN transcription in the tips of the embryonic cotyledons, which depends on the presence of canonical Auxin response elements (AuxREs), potential ARF-binding sites flanking the DRN transcription unit. Chromatin immunoprecipitation experiments show that MP binds in vivo to two AuxRE-spanning fragments in the DRN promoter, and that MP is required for expression of DRN in cotyledon tips. Hence, DRN represents a direct target of MP and functions downstream of MP in cotyledon development
Original languageEnglish
Pages (from-to)1643-1651
JournalDevelopment
Volume136
DOIs
Publication statusPublished - 2009

Keywords

  • cup-shaped-cotyledon
  • gene family-members
  • axis formation
  • vascular development
  • transcription factor
  • plant development
  • aux/iaa proteins
  • leaf formation
  • root-formation
  • cell fate

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