Does abscisic acis affect strigolactone biosynthesis?

J.A. Lopez-Raez, W. Kohlen, T. Charnikhova, P.P.J. Mulder, A.K. Undas, J. Sergeant, F. Verstappen, T.D.H. Bugg, A.J. Thompson, C.P. Ruyter-Spira, H.J. Bouwmeester

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117 Citations (Scopus)

Abstract

Strigolactones are considered a novel class of plant hormones that, in addition to their endogenous signalling function, are exuded into the rhizosphere acting as a signal to stimulate hyphal branching of arbuscular mycorrhizal (AM) fungi and germination of root parasitic plant seeds. Considering the importance of the strigolactones and their biosynthetic origin (from carotenoids), we investigated the relationship with the plant hormone abscisic acid (ABA). Strigolactone production and ABA content in the presence of specific inhibitors of oxidative carotenoid cleavage enzymes and in several tomato ABA-deficient mutants were analysed by LC-MS/MS. In addition, the expression of two genes involved in strigolactone biosynthesis was studied. The carotenoid cleavage dioxygenase (CCD) inhibitor D2 reduced strigolactone but not ABA content of roots. However, in abamineSG-treated plants, an inhibitor of 9-cis-epoxycarotenoid dioxygenase (NCED), and the ABA mutants notabilis, sitiens and flacca, ABA and strigolactones were greatly reduced. The reduction in strigolactone production correlated with the downregulation of LeCCD7 and LeCCD8 genes in all three mutants. The results show a correlation between ABA levels and strigolactone production, and suggest a role for ABA in the regulation of strigolactone biosynthesis.
Original languageEnglish
Pages (from-to)343-354
Number of pages12
JournalNew Phytologist
Volume187
Issue number2
DOIs
Publication statusPublished - 2010

Keywords

  • arbuscular mycorrhizal fungi
  • parasitic plants
  • germination stimulants
  • arabidopsis-thaliana
  • phosphorus stress
  • mutant notabilis
  • orobanche spp.
  • tomato plants
  • water-stress
  • wild-type

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    Lopez-Raez, J. A., Kohlen, W., Charnikhova, T., Mulder, P. P. J., Undas, A. K., Sergeant, J., Verstappen, F., Bugg, T. D. H., Thompson, A. J., Ruyter-Spira, C. P., & Bouwmeester, H. J. (2010). Does abscisic acis affect strigolactone biosynthesis? New Phytologist, 187(2), 343-354. https://doi.org/10.1111/j.1469-8137.2010.03291.x