DNA photolyases of Chrysodeixis chalcites nucleopolyhedrovirus are targeted to the nucleus and interact with chromosomes and mitotic spindle structures

F. Xu, J.M. Vlak, A.P.M. Eker, M.M. van Oers

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Cyclobutane pyrimidine dimer (CPD) photolyases convert UV-induced CPDs in DNA into monomers using visible light as the energy source. Two phr genes encoding class II CPD photolyases PHR1 and PHR2 have been identified in Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV). Transient expression assays in insect cells showed that PHR1–EGFP fusion protein was localized in the nucleus. Early after transfection, PHR2–EGFP was distributed over the cytoplasm and nucleus but, over time, it became localized predominantly in the nucleus. Immunofluorescence analysis with anti-PHR2 antiserum showed that, early after transfection, non-fused PHR2 was already present mainly in the nucleus, suggesting that the fusion of PHR2 to EGFP hindered its nuclear import. Both PHR–EGFP fusion proteins strongly colocalized with chromosomes and spindle, aster and midbody structures during host-cell mitosis. When PHR2–EGFP-transfected cells were superinfected with Autographa californica multiple-nucleocapsid NPV (AcMNPV), the protein colocalized with virogenic stroma, the replication factories of baculovirus DNA. The collective data support the supposition that the PHR2 protein plays a role in baculovirus DNA repair
Original languageEnglish
Pages (from-to)907-914
JournalJournal of General Virology
Volume91
Issue number4
DOIs
Publication statusPublished - 2010

Fingerprint

Deoxyribodipyrimidine Photo-Lyase
Nucleopolyhedrovirus
Spindle Apparatus
Pyrimidine Dimers
Chromosomes
Baculoviridae
Transfection
Nucleocapsid Proteins
MHC Class II Genes
Proteins
Cell Nucleus Active Transport
DNA
Mitosis
DNA Repair
Fluorescent Antibody Technique
Insects
Immune Sera
Cytoplasm
Light

Keywords

  • cyclobutane pyrimidine dimers
  • induced apoptosis
  • rna-polymerase
  • baculovirus
  • repair
  • identification
  • photorepair
  • irradiation
  • sequence
  • genome

Cite this

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title = "DNA photolyases of Chrysodeixis chalcites nucleopolyhedrovirus are targeted to the nucleus and interact with chromosomes and mitotic spindle structures",
abstract = "Cyclobutane pyrimidine dimer (CPD) photolyases convert UV-induced CPDs in DNA into monomers using visible light as the energy source. Two phr genes encoding class II CPD photolyases PHR1 and PHR2 have been identified in Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV). Transient expression assays in insect cells showed that PHR1–EGFP fusion protein was localized in the nucleus. Early after transfection, PHR2–EGFP was distributed over the cytoplasm and nucleus but, over time, it became localized predominantly in the nucleus. Immunofluorescence analysis with anti-PHR2 antiserum showed that, early after transfection, non-fused PHR2 was already present mainly in the nucleus, suggesting that the fusion of PHR2 to EGFP hindered its nuclear import. Both PHR–EGFP fusion proteins strongly colocalized with chromosomes and spindle, aster and midbody structures during host-cell mitosis. When PHR2–EGFP-transfected cells were superinfected with Autographa californica multiple-nucleocapsid NPV (AcMNPV), the protein colocalized with virogenic stroma, the replication factories of baculovirus DNA. The collective data support the supposition that the PHR2 protein plays a role in baculovirus DNA repair",
keywords = "cyclobutane pyrimidine dimers, induced apoptosis, rna-polymerase, baculovirus, repair, identification, photorepair, irradiation, sequence, genome",
author = "F. Xu and J.M. Vlak and A.P.M. Eker and {van Oers}, M.M.",
year = "2010",
doi = "10.1099/vir.0.018044-0",
language = "English",
volume = "91",
pages = "907--914",
journal = "Journal of General Virology",
issn = "0022-1317",
publisher = "Microbiology Society",
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}

DNA photolyases of Chrysodeixis chalcites nucleopolyhedrovirus are targeted to the nucleus and interact with chromosomes and mitotic spindle structures. / Xu, F.; Vlak, J.M.; Eker, A.P.M.; van Oers, M.M.

In: Journal of General Virology, Vol. 91, No. 4, 2010, p. 907-914.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - DNA photolyases of Chrysodeixis chalcites nucleopolyhedrovirus are targeted to the nucleus and interact with chromosomes and mitotic spindle structures

AU - Xu, F.

AU - Vlak, J.M.

AU - Eker, A.P.M.

AU - van Oers, M.M.

PY - 2010

Y1 - 2010

N2 - Cyclobutane pyrimidine dimer (CPD) photolyases convert UV-induced CPDs in DNA into monomers using visible light as the energy source. Two phr genes encoding class II CPD photolyases PHR1 and PHR2 have been identified in Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV). Transient expression assays in insect cells showed that PHR1–EGFP fusion protein was localized in the nucleus. Early after transfection, PHR2–EGFP was distributed over the cytoplasm and nucleus but, over time, it became localized predominantly in the nucleus. Immunofluorescence analysis with anti-PHR2 antiserum showed that, early after transfection, non-fused PHR2 was already present mainly in the nucleus, suggesting that the fusion of PHR2 to EGFP hindered its nuclear import. Both PHR–EGFP fusion proteins strongly colocalized with chromosomes and spindle, aster and midbody structures during host-cell mitosis. When PHR2–EGFP-transfected cells were superinfected with Autographa californica multiple-nucleocapsid NPV (AcMNPV), the protein colocalized with virogenic stroma, the replication factories of baculovirus DNA. The collective data support the supposition that the PHR2 protein plays a role in baculovirus DNA repair

AB - Cyclobutane pyrimidine dimer (CPD) photolyases convert UV-induced CPDs in DNA into monomers using visible light as the energy source. Two phr genes encoding class II CPD photolyases PHR1 and PHR2 have been identified in Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV). Transient expression assays in insect cells showed that PHR1–EGFP fusion protein was localized in the nucleus. Early after transfection, PHR2–EGFP was distributed over the cytoplasm and nucleus but, over time, it became localized predominantly in the nucleus. Immunofluorescence analysis with anti-PHR2 antiserum showed that, early after transfection, non-fused PHR2 was already present mainly in the nucleus, suggesting that the fusion of PHR2 to EGFP hindered its nuclear import. Both PHR–EGFP fusion proteins strongly colocalized with chromosomes and spindle, aster and midbody structures during host-cell mitosis. When PHR2–EGFP-transfected cells were superinfected with Autographa californica multiple-nucleocapsid NPV (AcMNPV), the protein colocalized with virogenic stroma, the replication factories of baculovirus DNA. The collective data support the supposition that the PHR2 protein plays a role in baculovirus DNA repair

KW - cyclobutane pyrimidine dimers

KW - induced apoptosis

KW - rna-polymerase

KW - baculovirus

KW - repair

KW - identification

KW - photorepair

KW - irradiation

KW - sequence

KW - genome

U2 - 10.1099/vir.0.018044-0

DO - 10.1099/vir.0.018044-0

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JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

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