The invention relates to the field of genetic engineering, and to the modification of nucleic acids and organismal genomes in particular. Specifically, the invention concerns the chemical modification of nucleic acids for the improved transformation of cells. Accordingly, the invention provides for modified nucleic acids, methods of modifying nucleic acids, methods of transforming cells and methods for the sequence-directed site-specific genetic modification of cells. The invention additionally encompasses cells transformed with modified nucleic acids and expression constructs for delivery and expression of modified nucleic acids. The nucleic acids, expression constructs, cells and methods of the invention find application in many areas of biotechnology, including, for example, genome editing.
|Publication status||Published - 6 Apr 2017|