DNA-guided DNA interference by a prokaryotic Argonaute

J. van der Oost (Inventor), D.C. Swarts (Inventor)

Research output: PatentOther research output

Abstract

This disclosure provides for compositions and methods for the use of designed nucleic acid-targeting nucleic acids, Argonautes, and complexes thereof. In one aspect, the disclosure provides for a composition comprising: a complex comprising: an Argonaute and a designed nucleic acid-targeting nucleic acid; and a target nucleic acid, wherein the designed nucleic acid-targeting nucleic acid is hybridized to the target nucleic acid. In some embodiments, the target nucleic acid is double-stranded. In some embodiments, the Argonaute comprises at least 30% amino acid identity to a prokaryotic Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to a bacterial Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an archaeal Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an Argonaute from a mesophile. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an Argonaute from a thermophile.
Original languageEnglish
Patent numberWO2014189628
Priority date11/04/13
Publication statusPublished - 27 Nov 2014

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Nucleic Acids
DNA
Amino Acids
Chemical analysis

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title = "DNA-guided DNA interference by a prokaryotic Argonaute",
abstract = "This disclosure provides for compositions and methods for the use of designed nucleic acid-targeting nucleic acids, Argonautes, and complexes thereof. In one aspect, the disclosure provides for a composition comprising: a complex comprising: an Argonaute and a designed nucleic acid-targeting nucleic acid; and a target nucleic acid, wherein the designed nucleic acid-targeting nucleic acid is hybridized to the target nucleic acid. In some embodiments, the target nucleic acid is double-stranded. In some embodiments, the Argonaute comprises at least 30{\%} amino acid identity to a prokaryotic Argonaute. In some embodiments, the Argonaute comprises at least 30{\%} amino acid identity to a bacterial Argonaute. In some embodiments, the Argonaute comprises at least 30{\%} amino acid identity to an archaeal Argonaute. In some embodiments, the Argonaute comprises at least 30{\%} amino acid identity to an Argonaute from a mesophile. In some embodiments, the Argonaute comprises at least 30{\%} amino acid identity to an Argonaute from a thermophile.",
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DNA-guided DNA interference by a prokaryotic Argonaute. / van der Oost, J. (Inventor); Swarts, D.C. (Inventor).

Patent No.: WO2014189628.

Research output: PatentOther research output

TY - PAT

T1 - DNA-guided DNA interference by a prokaryotic Argonaute

AU - van der Oost, J.

AU - Swarts, D.C.

PY - 2014/11/27

Y1 - 2014/11/27

N2 - This disclosure provides for compositions and methods for the use of designed nucleic acid-targeting nucleic acids, Argonautes, and complexes thereof. In one aspect, the disclosure provides for a composition comprising: a complex comprising: an Argonaute and a designed nucleic acid-targeting nucleic acid; and a target nucleic acid, wherein the designed nucleic acid-targeting nucleic acid is hybridized to the target nucleic acid. In some embodiments, the target nucleic acid is double-stranded. In some embodiments, the Argonaute comprises at least 30% amino acid identity to a prokaryotic Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to a bacterial Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an archaeal Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an Argonaute from a mesophile. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an Argonaute from a thermophile.

AB - This disclosure provides for compositions and methods for the use of designed nucleic acid-targeting nucleic acids, Argonautes, and complexes thereof. In one aspect, the disclosure provides for a composition comprising: a complex comprising: an Argonaute and a designed nucleic acid-targeting nucleic acid; and a target nucleic acid, wherein the designed nucleic acid-targeting nucleic acid is hybridized to the target nucleic acid. In some embodiments, the target nucleic acid is double-stranded. In some embodiments, the Argonaute comprises at least 30% amino acid identity to a prokaryotic Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to a bacterial Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an archaeal Argonaute. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an Argonaute from a mesophile. In some embodiments, the Argonaute comprises at least 30% amino acid identity to an Argonaute from a thermophile.

M3 - Patent

M1 - WO2014189628

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