TY - JOUR
T1 - DL-propargylglycine reduces blood pressure and renal injury but increases kidney weight in angiotensin-II infused rats
AU - Oosterhuis, Nynke R.
AU - Frenay, Anne Roos S.
AU - Wesseling, Sebas
AU - Snijder, Pauline M.
AU - Slaats, Gisela G.
AU - Yazdani, Saleh
AU - Fernandez, Bernadette O.
AU - Feelisch, Martin
AU - Giles, Rachel H.
AU - Verhaar, Marianne C.
AU - Joles, Jaap A.
AU - Van Goor, Harry
PY - 2015
Y1 - 2015
N2 - Hydrogen sulfide (H2S), carbon monoxide (CO) and nitric oxide (NO) share signaling and vasorelaxant properties and are involved in proliferation and apoptosis. Inhibiting NO production or availability induces hypertension and proteinuria, which is prevented by concomitant blockade of the H2S producing enzyme cystathionine γ-lyase (CSE) by d,l-propargylglycine (PAG). We hypothesized that blocking H2S production ameliorates Angiotensin II (AngII)-induced hypertension and renal injury in a rodent model. Effects of concomitant administration of PAG or saline were therefore studied in healthy (CON) and AngII hypertensive rats. In CON rats, PAG did not affect systolic blood pressure (SBP), but slightly increased proteinuria. In AngII rats PAG reduced SBP, proteinuria and plasma creatinine (180 ± 12 vs. 211 ± 19 mmHg; 66 ± 35 vs. 346 ± 92 mg/24 h; 24 ± 6 vs. 47 ± 15 μmol/L, respectively; p <0.01). Unexpectedly, kidney to body weight ratio was increased in all groups by PAG (p <0.05). Renal injury induced by AngII was reduced by PAG (p <0.001). HO-1 gene expression was increased by PAG alone (p <0.05). PAG increased inner cortical tubular cell proliferation after 1 week and decreased outer cortical tubular nucleus number/field after 4 weeks. In vitro proximal tubular cell size increased after exposure to PAG. In summary, blocking H2S production with PAG reduced SBP and renal injury in AngII infused rats. Independent of the cardiovascular and renal effects, PAG increased HO-1 gene expression and kidney weight. PAG alone increased tubular cell size and proliferation in-vivo and in-vitro. Our results are indicative of a complex interplay of gasotransmitter signaling/action of mutually compensatory nature in the kidney.
AB - Hydrogen sulfide (H2S), carbon monoxide (CO) and nitric oxide (NO) share signaling and vasorelaxant properties and are involved in proliferation and apoptosis. Inhibiting NO production or availability induces hypertension and proteinuria, which is prevented by concomitant blockade of the H2S producing enzyme cystathionine γ-lyase (CSE) by d,l-propargylglycine (PAG). We hypothesized that blocking H2S production ameliorates Angiotensin II (AngII)-induced hypertension and renal injury in a rodent model. Effects of concomitant administration of PAG or saline were therefore studied in healthy (CON) and AngII hypertensive rats. In CON rats, PAG did not affect systolic blood pressure (SBP), but slightly increased proteinuria. In AngII rats PAG reduced SBP, proteinuria and plasma creatinine (180 ± 12 vs. 211 ± 19 mmHg; 66 ± 35 vs. 346 ± 92 mg/24 h; 24 ± 6 vs. 47 ± 15 μmol/L, respectively; p <0.01). Unexpectedly, kidney to body weight ratio was increased in all groups by PAG (p <0.05). Renal injury induced by AngII was reduced by PAG (p <0.001). HO-1 gene expression was increased by PAG alone (p <0.05). PAG increased inner cortical tubular cell proliferation after 1 week and decreased outer cortical tubular nucleus number/field after 4 weeks. In vitro proximal tubular cell size increased after exposure to PAG. In summary, blocking H2S production with PAG reduced SBP and renal injury in AngII infused rats. Independent of the cardiovascular and renal effects, PAG increased HO-1 gene expression and kidney weight. PAG alone increased tubular cell size and proliferation in-vivo and in-vitro. Our results are indicative of a complex interplay of gasotransmitter signaling/action of mutually compensatory nature in the kidney.
KW - Angiotensin-II
KW - DL-Propargylglycine
KW - Hydrogen sulfide
KW - Hypertension
KW - Kidney weight
KW - Proteinuria
U2 - 10.1016/j.niox.2015.07.001
DO - 10.1016/j.niox.2015.07.001
M3 - Article
AN - SCOPUS:84938369656
VL - 49
SP - 56
EP - 66
JO - Nitric oxide : biology and chemistry
JF - Nitric oxide : biology and chemistry
SN - 1089-8603
ER -
