Diversity of tryptophan halogenases in sponges of the genus Aplysina

Johanna Gutleben, Jasper J. Koehorst, Kyle McPherson, Shirley Pomponi, René H. Wijffels, Hauke Smidt, Detmer Sipkema*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Marine sponges are a prolific source of novel enzymes with promising biotechnological potential. Especially halogenases, which are key enzymes in the biosynthesis of brominated and chlorinated secondary metabolites, possess interesting properties towards the production of pharmaceuticals that are often halogenated. In this study we used a polymerase chain reaction (PCR)-based screening to simultaneously examine and compare the richness and diversity of putative tryptophan halogenase protein sequences and bacterial community structures of six Aplysina species from the Mediterranean and Caribbean seas. At the phylum level, bacterial community composition was similar amongst all investigated species and predominated by Actinobacteria, Chloroflexi, Cyanobacteria, Gemmatimonadetes, and Proteobacteria. We detected four phylogenetically diverse clades of putative tryptophan halogenase protein sequences, which were only distantly related to previously reported halogenases. The Mediterranean species Aplysina aerophoba harbored unique halogenase sequences, of which the most predominant was related to a sponge-associated Psychrobacter-derived sequence. In contrast, the Caribbean species shared numerous novel halogenase sequence variants and exhibited a highly similar bacterial community composition at the operational taxonomic unit (OTU) level. Correlations of relative abundances of halogenases with those of bacterial taxa suggest that prominent sponge symbiotic bacteria, including Chloroflexi and Actinobacteria, are putative producers of the detected enzymes and may thus contribute to the chemical defense of their host.

Original languageEnglish
Article numberfiz108
JournalFEMS microbiology ecology
Volume95
Issue number8
DOIs
Publication statusPublished - 1 Aug 2019

Fingerprint

Porifera
Chloroflexi
sponge
Actinobacteria
Psychrobacter
Enzymes
enzyme
Mediterranean Sea
community composition
Bacterial Structures
Proteobacteria
Cyanobacteria
protein
chemical defense
secondary metabolite
Proteins
polymerase chain reaction
Bacteria
cyanobacterium
relative abundance

Keywords

  • bioactive compounds
  • environmental enzymes
  • Halogenase
  • host-associated microbiome
  • marine sponges
  • phylogenetic diversity

Cite this

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title = "Diversity of tryptophan halogenases in sponges of the genus Aplysina",
abstract = "Marine sponges are a prolific source of novel enzymes with promising biotechnological potential. Especially halogenases, which are key enzymes in the biosynthesis of brominated and chlorinated secondary metabolites, possess interesting properties towards the production of pharmaceuticals that are often halogenated. In this study we used a polymerase chain reaction (PCR)-based screening to simultaneously examine and compare the richness and diversity of putative tryptophan halogenase protein sequences and bacterial community structures of six Aplysina species from the Mediterranean and Caribbean seas. At the phylum level, bacterial community composition was similar amongst all investigated species and predominated by Actinobacteria, Chloroflexi, Cyanobacteria, Gemmatimonadetes, and Proteobacteria. We detected four phylogenetically diverse clades of putative tryptophan halogenase protein sequences, which were only distantly related to previously reported halogenases. The Mediterranean species Aplysina aerophoba harbored unique halogenase sequences, of which the most predominant was related to a sponge-associated Psychrobacter-derived sequence. In contrast, the Caribbean species shared numerous novel halogenase sequence variants and exhibited a highly similar bacterial community composition at the operational taxonomic unit (OTU) level. Correlations of relative abundances of halogenases with those of bacterial taxa suggest that prominent sponge symbiotic bacteria, including Chloroflexi and Actinobacteria, are putative producers of the detected enzymes and may thus contribute to the chemical defense of their host.",
keywords = "bioactive compounds, environmental enzymes, Halogenase, host-associated microbiome, marine sponges, phylogenetic diversity",
author = "Johanna Gutleben and Koehorst, {Jasper J.} and Kyle McPherson and Shirley Pomponi and Wijffels, {Ren{\'e} H.} and Hauke Smidt and Detmer Sipkema",
year = "2019",
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language = "English",
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Diversity of tryptophan halogenases in sponges of the genus Aplysina. / Gutleben, Johanna; Koehorst, Jasper J.; McPherson, Kyle; Pomponi, Shirley; Wijffels, René H.; Smidt, Hauke; Sipkema, Detmer.

In: FEMS microbiology ecology, Vol. 95, No. 8, fiz108, 01.08.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Diversity of tryptophan halogenases in sponges of the genus Aplysina

AU - Gutleben, Johanna

AU - Koehorst, Jasper J.

AU - McPherson, Kyle

AU - Pomponi, Shirley

AU - Wijffels, René H.

AU - Smidt, Hauke

AU - Sipkema, Detmer

PY - 2019/8/1

Y1 - 2019/8/1

N2 - Marine sponges are a prolific source of novel enzymes with promising biotechnological potential. Especially halogenases, which are key enzymes in the biosynthesis of brominated and chlorinated secondary metabolites, possess interesting properties towards the production of pharmaceuticals that are often halogenated. In this study we used a polymerase chain reaction (PCR)-based screening to simultaneously examine and compare the richness and diversity of putative tryptophan halogenase protein sequences and bacterial community structures of six Aplysina species from the Mediterranean and Caribbean seas. At the phylum level, bacterial community composition was similar amongst all investigated species and predominated by Actinobacteria, Chloroflexi, Cyanobacteria, Gemmatimonadetes, and Proteobacteria. We detected four phylogenetically diverse clades of putative tryptophan halogenase protein sequences, which were only distantly related to previously reported halogenases. The Mediterranean species Aplysina aerophoba harbored unique halogenase sequences, of which the most predominant was related to a sponge-associated Psychrobacter-derived sequence. In contrast, the Caribbean species shared numerous novel halogenase sequence variants and exhibited a highly similar bacterial community composition at the operational taxonomic unit (OTU) level. Correlations of relative abundances of halogenases with those of bacterial taxa suggest that prominent sponge symbiotic bacteria, including Chloroflexi and Actinobacteria, are putative producers of the detected enzymes and may thus contribute to the chemical defense of their host.

AB - Marine sponges are a prolific source of novel enzymes with promising biotechnological potential. Especially halogenases, which are key enzymes in the biosynthesis of brominated and chlorinated secondary metabolites, possess interesting properties towards the production of pharmaceuticals that are often halogenated. In this study we used a polymerase chain reaction (PCR)-based screening to simultaneously examine and compare the richness and diversity of putative tryptophan halogenase protein sequences and bacterial community structures of six Aplysina species from the Mediterranean and Caribbean seas. At the phylum level, bacterial community composition was similar amongst all investigated species and predominated by Actinobacteria, Chloroflexi, Cyanobacteria, Gemmatimonadetes, and Proteobacteria. We detected four phylogenetically diverse clades of putative tryptophan halogenase protein sequences, which were only distantly related to previously reported halogenases. The Mediterranean species Aplysina aerophoba harbored unique halogenase sequences, of which the most predominant was related to a sponge-associated Psychrobacter-derived sequence. In contrast, the Caribbean species shared numerous novel halogenase sequence variants and exhibited a highly similar bacterial community composition at the operational taxonomic unit (OTU) level. Correlations of relative abundances of halogenases with those of bacterial taxa suggest that prominent sponge symbiotic bacteria, including Chloroflexi and Actinobacteria, are putative producers of the detected enzymes and may thus contribute to the chemical defense of their host.

KW - bioactive compounds

KW - environmental enzymes

KW - Halogenase

KW - host-associated microbiome

KW - marine sponges

KW - phylogenetic diversity

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