Diversity of STs, plasmids and ESBL genes among Escherichia coli from humans, animals and food in Germany, the Netherlands and the UK

Michaela J. Day, Irene Rodríguez, Alieda van Essen-Zandbergen, Cindy Dierikx, Kristina Kadlec, Anne Kathrin Schink, Guanghui Wu, Marie A. Chattaway, Vivienne DoNascimento, John Wain, Reiner Helmuth, Beatriz Guerra, Stefan Schwarz, John Threlfall, Martin J. Woodward, Nick Coldham, Dik Mevius, Neil Woodford

Research output: Contribution to journalArticleAcademicpeer-review

46 Citations (Scopus)

Abstract

Objectives: This study aimed to compare ESBL-producing Escherichia coli causing infections in humans with infecting or commensal isolates from animals and isolates from food of animal origin in terms of the strain types, the ESBL gene present and the plasmids that carry the respective ESBL genes. Methods: A collection of 353 ESBL-positive E. coli isolates from the UK, the Netherlands and Germany were studied by MLST and ESBL genes were identified. Characterization of ESBL gene-carrying plasmids was performed using PCR-based replicon typing. Moreover, IncI1-Iγ and IncN plasmids were characterized by plasmid MLST. Results: The ESBL-producing E. coli represented 158 different STs with ST131, ST10 and ST88 being the most common. Overall, blaCTX-M-1 was the most frequently detected ESBL gene, followed by blaCTX-M-15, which was the most common ESBL gene in the human isolates. The most common plasmid replicon type overall was IncI1-Ig followed by multiple IncF replicons. Conclusions: ESBL genes were present in a wide variety of E. coli STs. IncI1-Iγ plasmids that carried the blaCTX-M-1 gene were widely disseminated amongst STs in isolates from animals and humans, whereas other plasmids and STs appeared to be more restricted to isolates from specific hosts.

Original languageEnglish
Article numberdkv485
Pages (from-to)1178-1182
JournalJournal of Antimicrobial Chemotherapy
Volume71
Issue number5
DOIs
Publication statusPublished - 2016

Fingerprint

Netherlands
Germany
Plasmids
Escherichia coli
Food
Replicon
Genes
Escherichia coli Infections
Polymerase Chain Reaction

Cite this

Day, M. J., Rodríguez, I., van Essen-Zandbergen, A., Dierikx, C., Kadlec, K., Schink, A. K., ... Woodford, N. (2016). Diversity of STs, plasmids and ESBL genes among Escherichia coli from humans, animals and food in Germany, the Netherlands and the UK. Journal of Antimicrobial Chemotherapy, 71(5), 1178-1182. [dkv485]. https://doi.org/10.1093/jac/dkv485
Day, Michaela J. ; Rodríguez, Irene ; van Essen-Zandbergen, Alieda ; Dierikx, Cindy ; Kadlec, Kristina ; Schink, Anne Kathrin ; Wu, Guanghui ; Chattaway, Marie A. ; DoNascimento, Vivienne ; Wain, John ; Helmuth, Reiner ; Guerra, Beatriz ; Schwarz, Stefan ; Threlfall, John ; Woodward, Martin J. ; Coldham, Nick ; Mevius, Dik ; Woodford, Neil. / Diversity of STs, plasmids and ESBL genes among Escherichia coli from humans, animals and food in Germany, the Netherlands and the UK. In: Journal of Antimicrobial Chemotherapy. 2016 ; Vol. 71, No. 5. pp. 1178-1182.
@article{ea81c86cb6fd4a67b3e42216242e9dd1,
title = "Diversity of STs, plasmids and ESBL genes among Escherichia coli from humans, animals and food in Germany, the Netherlands and the UK",
abstract = "Objectives: This study aimed to compare ESBL-producing Escherichia coli causing infections in humans with infecting or commensal isolates from animals and isolates from food of animal origin in terms of the strain types, the ESBL gene present and the plasmids that carry the respective ESBL genes. Methods: A collection of 353 ESBL-positive E. coli isolates from the UK, the Netherlands and Germany were studied by MLST and ESBL genes were identified. Characterization of ESBL gene-carrying plasmids was performed using PCR-based replicon typing. Moreover, IncI1-Iγ and IncN plasmids were characterized by plasmid MLST. Results: The ESBL-producing E. coli represented 158 different STs with ST131, ST10 and ST88 being the most common. Overall, blaCTX-M-1 was the most frequently detected ESBL gene, followed by blaCTX-M-15, which was the most common ESBL gene in the human isolates. The most common plasmid replicon type overall was IncI1-Ig followed by multiple IncF replicons. Conclusions: ESBL genes were present in a wide variety of E. coli STs. IncI1-Iγ plasmids that carried the blaCTX-M-1 gene were widely disseminated amongst STs in isolates from animals and humans, whereas other plasmids and STs appeared to be more restricted to isolates from specific hosts.",
author = "Day, {Michaela J.} and Irene Rodr{\'i}guez and {van Essen-Zandbergen}, Alieda and Cindy Dierikx and Kristina Kadlec and Schink, {Anne Kathrin} and Guanghui Wu and Chattaway, {Marie A.} and Vivienne DoNascimento and John Wain and Reiner Helmuth and Beatriz Guerra and Stefan Schwarz and John Threlfall and Woodward, {Martin J.} and Nick Coldham and Dik Mevius and Neil Woodford",
year = "2016",
doi = "10.1093/jac/dkv485",
language = "English",
volume = "71",
pages = "1178--1182",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "5",

}

Day, MJ, Rodríguez, I, van Essen-Zandbergen, A, Dierikx, C, Kadlec, K, Schink, AK, Wu, G, Chattaway, MA, DoNascimento, V, Wain, J, Helmuth, R, Guerra, B, Schwarz, S, Threlfall, J, Woodward, MJ, Coldham, N, Mevius, D & Woodford, N 2016, 'Diversity of STs, plasmids and ESBL genes among Escherichia coli from humans, animals and food in Germany, the Netherlands and the UK' Journal of Antimicrobial Chemotherapy, vol. 71, no. 5, dkv485, pp. 1178-1182. https://doi.org/10.1093/jac/dkv485

Diversity of STs, plasmids and ESBL genes among Escherichia coli from humans, animals and food in Germany, the Netherlands and the UK. / Day, Michaela J.; Rodríguez, Irene; van Essen-Zandbergen, Alieda; Dierikx, Cindy; Kadlec, Kristina; Schink, Anne Kathrin; Wu, Guanghui; Chattaway, Marie A.; DoNascimento, Vivienne; Wain, John; Helmuth, Reiner; Guerra, Beatriz; Schwarz, Stefan; Threlfall, John; Woodward, Martin J.; Coldham, Nick; Mevius, Dik; Woodford, Neil.

In: Journal of Antimicrobial Chemotherapy, Vol. 71, No. 5, dkv485, 2016, p. 1178-1182.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Diversity of STs, plasmids and ESBL genes among Escherichia coli from humans, animals and food in Germany, the Netherlands and the UK

AU - Day, Michaela J.

AU - Rodríguez, Irene

AU - van Essen-Zandbergen, Alieda

AU - Dierikx, Cindy

AU - Kadlec, Kristina

AU - Schink, Anne Kathrin

AU - Wu, Guanghui

AU - Chattaway, Marie A.

AU - DoNascimento, Vivienne

AU - Wain, John

AU - Helmuth, Reiner

AU - Guerra, Beatriz

AU - Schwarz, Stefan

AU - Threlfall, John

AU - Woodward, Martin J.

AU - Coldham, Nick

AU - Mevius, Dik

AU - Woodford, Neil

PY - 2016

Y1 - 2016

N2 - Objectives: This study aimed to compare ESBL-producing Escherichia coli causing infections in humans with infecting or commensal isolates from animals and isolates from food of animal origin in terms of the strain types, the ESBL gene present and the plasmids that carry the respective ESBL genes. Methods: A collection of 353 ESBL-positive E. coli isolates from the UK, the Netherlands and Germany were studied by MLST and ESBL genes were identified. Characterization of ESBL gene-carrying plasmids was performed using PCR-based replicon typing. Moreover, IncI1-Iγ and IncN plasmids were characterized by plasmid MLST. Results: The ESBL-producing E. coli represented 158 different STs with ST131, ST10 and ST88 being the most common. Overall, blaCTX-M-1 was the most frequently detected ESBL gene, followed by blaCTX-M-15, which was the most common ESBL gene in the human isolates. The most common plasmid replicon type overall was IncI1-Ig followed by multiple IncF replicons. Conclusions: ESBL genes were present in a wide variety of E. coli STs. IncI1-Iγ plasmids that carried the blaCTX-M-1 gene were widely disseminated amongst STs in isolates from animals and humans, whereas other plasmids and STs appeared to be more restricted to isolates from specific hosts.

AB - Objectives: This study aimed to compare ESBL-producing Escherichia coli causing infections in humans with infecting or commensal isolates from animals and isolates from food of animal origin in terms of the strain types, the ESBL gene present and the plasmids that carry the respective ESBL genes. Methods: A collection of 353 ESBL-positive E. coli isolates from the UK, the Netherlands and Germany were studied by MLST and ESBL genes were identified. Characterization of ESBL gene-carrying plasmids was performed using PCR-based replicon typing. Moreover, IncI1-Iγ and IncN plasmids were characterized by plasmid MLST. Results: The ESBL-producing E. coli represented 158 different STs with ST131, ST10 and ST88 being the most common. Overall, blaCTX-M-1 was the most frequently detected ESBL gene, followed by blaCTX-M-15, which was the most common ESBL gene in the human isolates. The most common plasmid replicon type overall was IncI1-Ig followed by multiple IncF replicons. Conclusions: ESBL genes were present in a wide variety of E. coli STs. IncI1-Iγ plasmids that carried the blaCTX-M-1 gene were widely disseminated amongst STs in isolates from animals and humans, whereas other plasmids and STs appeared to be more restricted to isolates from specific hosts.

U2 - 10.1093/jac/dkv485

DO - 10.1093/jac/dkv485

M3 - Article

VL - 71

SP - 1178

EP - 1182

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 5

M1 - dkv485

ER -