Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in Cerrado soil

I. von der Weid, E. Paiva, A. Nobrega, J.D. van Elsas, L. Seldin

    Research output: Contribution to journalArticleAcademicpeer-review

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    Abstract

    Paenibacillus polymyxa populations present in the rhizosphere of maize (cultivar BR-201) planted in Cerrado soil were investigated in order to assess their diversity at four stages of plant growth. A total of 67 strains were isolated and all strains were identified as P. polymyxa by classical biochemical tests, API 50CH tests and a set of species-specific primers based on the 23S rDNA sequence. To compare the isolated strains, phenotypic characteristics (utilization of different carbohydrates, resistance to antibiotics and production of antimicrobial substances) and genetic approaches (hybridization with a Klebsiella pneumoniae nifKDH probe and BOX- PCR) were used. Fermentation of glycerol, arabinose, xylose and rhamnose varied among the isolates and these data divided the strains into five groups. Fifty strains (75%) showed homology to plasmid pSA30 (containing the nifKDH genes) resulting in five different hybridization patterns. Using BOX- PCR, 18 groups were observed. Phenetic analyses were applied based on the unweighted pair group method with arithmetic means using the phenotypic and genetic data, separately. All P. polymyxa isolates could be divided into two main clusters at approximately 52% and into 18 groups at approximately 89% of similarity, when phenotypic data were used. Also, two main clusters were formed at 65% of similarity when genetic data were used. In this dendrogram, clusters were further split into 10 and 22 groups, at about 88 and 97% of similarity, respectively. Finally, all phenotypic and genetic data, or just the genetic data, were used in a multivariate analysis of variance (MANOVA) in order to address the heterogeneity among P. polymyxa populations during the different stages of maize growth. The resulting data showed that strains isolated 10, 30, 60 and 90 days after maize sowing were statistically different. (C) 2000 Editions scientifiques et medicales Elsevier SAS.
    Original languageEnglish
    Pages (from-to)369-381
    JournalResearch in Microbiology
    Volume151
    Issue number5
    DOIs
    Publication statusPublished - 2000

    Fingerprint

    Rhizosphere
    Zea mays
    Soil
    Genetic Hybridization
    Rhamnose
    Polymerase Chain Reaction
    Arabinose
    Xylose
    Klebsiella pneumoniae
    Microbial Drug Resistance
    Growth
    Ribosomal DNA
    Glycerol
    Population
    Fermentation
    Analysis of Variance
    Plasmids
    Multivariate Analysis
    Carbohydrates
    Paenibacillus polymyxa

    Cite this

    von der Weid, I. ; Paiva, E. ; Nobrega, A. ; van Elsas, J.D. ; Seldin, L. / Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in Cerrado soil. In: Research in Microbiology. 2000 ; Vol. 151, No. 5. pp. 369-381.
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    abstract = "Paenibacillus polymyxa populations present in the rhizosphere of maize (cultivar BR-201) planted in Cerrado soil were investigated in order to assess their diversity at four stages of plant growth. A total of 67 strains were isolated and all strains were identified as P. polymyxa by classical biochemical tests, API 50CH tests and a set of species-specific primers based on the 23S rDNA sequence. To compare the isolated strains, phenotypic characteristics (utilization of different carbohydrates, resistance to antibiotics and production of antimicrobial substances) and genetic approaches (hybridization with a Klebsiella pneumoniae nifKDH probe and BOX- PCR) were used. Fermentation of glycerol, arabinose, xylose and rhamnose varied among the isolates and these data divided the strains into five groups. Fifty strains (75{\%}) showed homology to plasmid pSA30 (containing the nifKDH genes) resulting in five different hybridization patterns. Using BOX- PCR, 18 groups were observed. Phenetic analyses were applied based on the unweighted pair group method with arithmetic means using the phenotypic and genetic data, separately. All P. polymyxa isolates could be divided into two main clusters at approximately 52{\%} and into 18 groups at approximately 89{\%} of similarity, when phenotypic data were used. Also, two main clusters were formed at 65{\%} of similarity when genetic data were used. In this dendrogram, clusters were further split into 10 and 22 groups, at about 88 and 97{\%} of similarity, respectively. Finally, all phenotypic and genetic data, or just the genetic data, were used in a multivariate analysis of variance (MANOVA) in order to address the heterogeneity among P. polymyxa populations during the different stages of maize growth. The resulting data showed that strains isolated 10, 30, 60 and 90 days after maize sowing were statistically different. (C) 2000 Editions scientifiques et medicales Elsevier SAS.",
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    Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in Cerrado soil. / von der Weid, I.; Paiva, E.; Nobrega, A.; van Elsas, J.D.; Seldin, L.

    In: Research in Microbiology, Vol. 151, No. 5, 2000, p. 369-381.

    Research output: Contribution to journalArticleAcademicpeer-review

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