Diversity in secondary metabolites including mycotoxins from strains of aspergillus section nigri isolated from raw cashew nuts from benin, west africa

Yendouban Lamboni, Kristian F. Nielsen, Anita R. Linnemann, Yué Ksel Gezgin, Kerstin Hell, Rob Nout, Eddy J. Smid, Manuele Tamo, M.A.J.S. van Boekel, Jakob Blñsbjerg Hoof, Jens Christian Frisvad

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Abstract

In a previous study, raw cashew kernels were assayed for the fungal contamination focusin on strains belonging to the genus Aspergillus and on aflatoxins producers. These sample showed high contamination with Aspergillus section Nigri species and absence o aflatoxins. To investigate the diversity of secondary metabolites, including mycotoxins, th species of A. section Nigri may produce and thus threaten to contaminate the raw cashe kernels, 150 strains were isolated from cashew samples and assayed for their productio of secondary metabolites using liquid chromatography high resolution mass spectrometr (LC-HRMS). Seven species of black Aspergilli were isolated based on morphological an chemical identification: A.Tubingensis (44%), A. niger (32%), A. brasiliensis (10%), A. carbonariu (8.7%), A. luchuensis (2.7%), A. aculeatus (2%) and A. aculeatinus (0.7%). Fro these, 45 metabolites and their isomers were identified. Aurasperone and pyranonigrin A produced by all species excluding A. aculeatus and A. aculeatinus, were most prevalen and were encountered in 146 (97.3%) and 145 (95.7%) isolates, respectively. Three mycotoxin groups were detected: fumonisins (B2 and B4) (2.7%) ochratoxin A (13.3%), an secalonic acids (2%), indicating that these mycotoxins could occur in raw cashew nuts Thirty strains of black Aspergilli were randomly sampled for verification of species identit based on sequences of β-Tubulin and calmodulin genes. Among them, 27 isolates wer positive to the primers used and 11 were identified as A. niger, 7 as A.Tubingensis, 6 as A carbonarius, 2 as A. luchuensis and 1 as A. welwitschiae confirming the species names a based on morphology and chemical features. These strains clustered in 5 clades in A. sectio Nigri. Chemical profile clustering also showed also 5 groups confirming the speciespecific metabolites production.

Original languageEnglish
Article numbere0164310
JournalPLoS ONE
Volume11
Issue number10
DOIs
Publication statusPublished - 2016

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Anacardium
Benin
cashew nuts
Nuts
Western Africa
Mycotoxins
Aspergillus
Metabolites
mycotoxins
secondary metabolites
Aflatoxins
aflatoxins
fumonisin B4
Contamination
fumonisin B2
metabolites
Liquid chromatography
ochratoxin A
calmodulin
Calmodulin

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Lamboni, Yendouban ; Nielsen, Kristian F. ; Linnemann, Anita R. ; Gezgin, Yué Ksel ; Hell, Kerstin ; Nout, Rob ; Smid, Eddy J. ; Tamo, Manuele ; van Boekel, M.A.J.S. ; Hoof, Jakob Blñsbjerg ; Frisvad, Jens Christian. / Diversity in secondary metabolites including mycotoxins from strains of aspergillus section nigri isolated from raw cashew nuts from benin, west africa. In: PLoS ONE. 2016 ; Vol. 11, No. 10.
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abstract = "In a previous study, raw cashew kernels were assayed for the fungal contamination focusin on strains belonging to the genus Aspergillus and on aflatoxins producers. These sample showed high contamination with Aspergillus section Nigri species and absence o aflatoxins. To investigate the diversity of secondary metabolites, including mycotoxins, th species of A. section Nigri may produce and thus threaten to contaminate the raw cashe kernels, 150 strains were isolated from cashew samples and assayed for their productio of secondary metabolites using liquid chromatography high resolution mass spectrometr (LC-HRMS). Seven species of black Aspergilli were isolated based on morphological an chemical identification: A.Tubingensis (44{\%}), A. niger (32{\%}), A. brasiliensis (10{\%}), A. carbonariu (8.7{\%}), A. luchuensis (2.7{\%}), A. aculeatus (2{\%}) and A. aculeatinus (0.7{\%}). Fro these, 45 metabolites and their isomers were identified. Aurasperone and pyranonigrin A produced by all species excluding A. aculeatus and A. aculeatinus, were most prevalen and were encountered in 146 (97.3{\%}) and 145 (95.7{\%}) isolates, respectively. Three mycotoxin groups were detected: fumonisins (B2 and B4) (2.7{\%}) ochratoxin A (13.3{\%}), an secalonic acids (2{\%}), indicating that these mycotoxins could occur in raw cashew nuts Thirty strains of black Aspergilli were randomly sampled for verification of species identit based on sequences of β-Tubulin and calmodulin genes. Among them, 27 isolates wer positive to the primers used and 11 were identified as A. niger, 7 as A.Tubingensis, 6 as A carbonarius, 2 as A. luchuensis and 1 as A. welwitschiae confirming the species names a based on morphology and chemical features. These strains clustered in 5 clades in A. sectio Nigri. Chemical profile clustering also showed also 5 groups confirming the speciespecific metabolites production.",
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Diversity in secondary metabolites including mycotoxins from strains of aspergillus section nigri isolated from raw cashew nuts from benin, west africa. / Lamboni, Yendouban; Nielsen, Kristian F.; Linnemann, Anita R.; Gezgin, Yué Ksel; Hell, Kerstin; Nout, Rob; Smid, Eddy J.; Tamo, Manuele; van Boekel, M.A.J.S.; Hoof, Jakob Blñsbjerg; Frisvad, Jens Christian.

In: PLoS ONE, Vol. 11, No. 10, e0164310, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Diversity in secondary metabolites including mycotoxins from strains of aspergillus section nigri isolated from raw cashew nuts from benin, west africa

AU - Lamboni, Yendouban

AU - Nielsen, Kristian F.

AU - Linnemann, Anita R.

AU - Gezgin, Yué Ksel

AU - Hell, Kerstin

AU - Nout, Rob

AU - Smid, Eddy J.

AU - Tamo, Manuele

AU - van Boekel, M.A.J.S.

AU - Hoof, Jakob Blñsbjerg

AU - Frisvad, Jens Christian

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AB - In a previous study, raw cashew kernels were assayed for the fungal contamination focusin on strains belonging to the genus Aspergillus and on aflatoxins producers. These sample showed high contamination with Aspergillus section Nigri species and absence o aflatoxins. To investigate the diversity of secondary metabolites, including mycotoxins, th species of A. section Nigri may produce and thus threaten to contaminate the raw cashe kernels, 150 strains were isolated from cashew samples and assayed for their productio of secondary metabolites using liquid chromatography high resolution mass spectrometr (LC-HRMS). Seven species of black Aspergilli were isolated based on morphological an chemical identification: A.Tubingensis (44%), A. niger (32%), A. brasiliensis (10%), A. carbonariu (8.7%), A. luchuensis (2.7%), A. aculeatus (2%) and A. aculeatinus (0.7%). Fro these, 45 metabolites and their isomers were identified. Aurasperone and pyranonigrin A produced by all species excluding A. aculeatus and A. aculeatinus, were most prevalen and were encountered in 146 (97.3%) and 145 (95.7%) isolates, respectively. Three mycotoxin groups were detected: fumonisins (B2 and B4) (2.7%) ochratoxin A (13.3%), an secalonic acids (2%), indicating that these mycotoxins could occur in raw cashew nuts Thirty strains of black Aspergilli were randomly sampled for verification of species identit based on sequences of β-Tubulin and calmodulin genes. Among them, 27 isolates wer positive to the primers used and 11 were identified as A. niger, 7 as A.Tubingensis, 6 as A carbonarius, 2 as A. luchuensis and 1 as A. welwitschiae confirming the species names a based on morphology and chemical features. These strains clustered in 5 clades in A. sectio Nigri. Chemical profile clustering also showed also 5 groups confirming the speciespecific metabolites production.

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