Ditr P79_Characterisation of PLETHORA during gall formation of the root-knot nematode Meloidogyne incognita

Research output: Contribution to conferencePosterAcademic

Abstract

The parasitic root-knot nematode Meloidogyne incognita manipulates developmental processes in plants through formation of large tumour-like structures, designated as galls, in the roots of their hosts. The transcriptional profile of these galls show significant overlap with that of the formation of lateral roots (Cabrera et al., 2014). Key regulators in the formation of lateral roots are the PLETHORA (PLT) transcription factors PLT3, PLT5 and PLT7. They are induced by the sustained presence of the phytohormone auxin. These genes work together to regulate positioning and outgrowth of lateral root primordia (Hofhuis et al., 2013). The auxin efflux proteins PINFORMED (PIN) are thought to play an important role in this process and PLTs are known to regulate them in plants (Prasad et al., 2011). During the initial development of nematode-induced galls an accumulation of auxin is observed (Karczmarek et al., 2004). Additionally PIN proteins are known to be involved in gall formation (Kyndt et al., 2016). However, how the PIN proteins are regulated upon root-knot nematode infection is unknown. Hence, we studied the possibility that the PLT-PIN module is used during the formation of feeding sites of M. incognita. Mutant lines of the PLT genes showed a decrease in the number of infections and in number of reproducing females as compared to the wild type lines. Additionally, in several mutant lines galls developed that were significantly increased in size. Moreover, the auxin exporter PIN3 in the mutant line of the three PLT genes was no longer localised at the plasma membranes of cells within the feeding site in contrast to the wild type lines. We conclude that the three PLT genes are involved in feeding site initiation and development and could potentially achieve this through regulation of PIN.
Original languageEnglish
Publication statusPublished - 11 Sep 2018
EventESN Conference 2018 - Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
Duration: 9 Sep 201813 Sep 2018

Conference

ConferenceESN Conference 2018
CountryBelgium
CityGhent
Period9/09/1813/09/18

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Meloidogyne incognita
root-knot nematodes
galls
auxins
mutants
genes
root galls
root primordia
proteins
nematode infections
plant hormones
plasma membrane
transcription factors
Nematoda
neoplasms
infection
cells

Cite this

@conference{5e9cbf8dadc64827bfbcd1510a589a7e,
title = "Ditr P79_Characterisation of PLETHORA during gall formation of the root-knot nematode Meloidogyne incognita",
abstract = "The parasitic root-knot nematode Meloidogyne incognita manipulates developmental processes in plants through formation of large tumour-like structures, designated as galls, in the roots of their hosts. The transcriptional profile of these galls show significant overlap with that of the formation of lateral roots (Cabrera et al., 2014). Key regulators in the formation of lateral roots are the PLETHORA (PLT) transcription factors PLT3, PLT5 and PLT7. They are induced by the sustained presence of the phytohormone auxin. These genes work together to regulate positioning and outgrowth of lateral root primordia (Hofhuis et al., 2013). The auxin efflux proteins PINFORMED (PIN) are thought to play an important role in this process and PLTs are known to regulate them in plants (Prasad et al., 2011). During the initial development of nematode-induced galls an accumulation of auxin is observed (Karczmarek et al., 2004). Additionally PIN proteins are known to be involved in gall formation (Kyndt et al., 2016). However, how the PIN proteins are regulated upon root-knot nematode infection is unknown. Hence, we studied the possibility that the PLT-PIN module is used during the formation of feeding sites of M. incognita. Mutant lines of the PLT genes showed a decrease in the number of infections and in number of reproducing females as compared to the wild type lines. Additionally, in several mutant lines galls developed that were significantly increased in size. Moreover, the auxin exporter PIN3 in the mutant line of the three PLT genes was no longer localised at the plasma membranes of cells within the feeding site in contrast to the wild type lines. We conclude that the three PLT genes are involved in feeding site initiation and development and could potentially achieve this through regulation of PIN.",
author = "Matthijs Oosterbeek and H.A. Overmars and B.J.G. Scheres and J. Bakker and A. Goverse",
year = "2018",
month = "9",
day = "11",
language = "English",
note = "ESN Conference 2018 ; Conference date: 09-09-2018 Through 13-09-2018",

}

Ditr P79_Characterisation of PLETHORA during gall formation of the root-knot nematode Meloidogyne incognita. / Oosterbeek, Matthijs; Overmars, H.A.; Scheres, B.J.G.; Bakker, J.; Goverse, A.

2018. Poster session presented at ESN Conference 2018, Ghent, Belgium.

Research output: Contribution to conferencePosterAcademic

TY - CONF

T1 - Ditr P79_Characterisation of PLETHORA during gall formation of the root-knot nematode Meloidogyne incognita

AU - Oosterbeek, Matthijs

AU - Overmars, H.A.

AU - Scheres, B.J.G.

AU - Bakker, J.

AU - Goverse, A.

PY - 2018/9/11

Y1 - 2018/9/11

N2 - The parasitic root-knot nematode Meloidogyne incognita manipulates developmental processes in plants through formation of large tumour-like structures, designated as galls, in the roots of their hosts. The transcriptional profile of these galls show significant overlap with that of the formation of lateral roots (Cabrera et al., 2014). Key regulators in the formation of lateral roots are the PLETHORA (PLT) transcription factors PLT3, PLT5 and PLT7. They are induced by the sustained presence of the phytohormone auxin. These genes work together to regulate positioning and outgrowth of lateral root primordia (Hofhuis et al., 2013). The auxin efflux proteins PINFORMED (PIN) are thought to play an important role in this process and PLTs are known to regulate them in plants (Prasad et al., 2011). During the initial development of nematode-induced galls an accumulation of auxin is observed (Karczmarek et al., 2004). Additionally PIN proteins are known to be involved in gall formation (Kyndt et al., 2016). However, how the PIN proteins are regulated upon root-knot nematode infection is unknown. Hence, we studied the possibility that the PLT-PIN module is used during the formation of feeding sites of M. incognita. Mutant lines of the PLT genes showed a decrease in the number of infections and in number of reproducing females as compared to the wild type lines. Additionally, in several mutant lines galls developed that were significantly increased in size. Moreover, the auxin exporter PIN3 in the mutant line of the three PLT genes was no longer localised at the plasma membranes of cells within the feeding site in contrast to the wild type lines. We conclude that the three PLT genes are involved in feeding site initiation and development and could potentially achieve this through regulation of PIN.

AB - The parasitic root-knot nematode Meloidogyne incognita manipulates developmental processes in plants through formation of large tumour-like structures, designated as galls, in the roots of their hosts. The transcriptional profile of these galls show significant overlap with that of the formation of lateral roots (Cabrera et al., 2014). Key regulators in the formation of lateral roots are the PLETHORA (PLT) transcription factors PLT3, PLT5 and PLT7. They are induced by the sustained presence of the phytohormone auxin. These genes work together to regulate positioning and outgrowth of lateral root primordia (Hofhuis et al., 2013). The auxin efflux proteins PINFORMED (PIN) are thought to play an important role in this process and PLTs are known to regulate them in plants (Prasad et al., 2011). During the initial development of nematode-induced galls an accumulation of auxin is observed (Karczmarek et al., 2004). Additionally PIN proteins are known to be involved in gall formation (Kyndt et al., 2016). However, how the PIN proteins are regulated upon root-knot nematode infection is unknown. Hence, we studied the possibility that the PLT-PIN module is used during the formation of feeding sites of M. incognita. Mutant lines of the PLT genes showed a decrease in the number of infections and in number of reproducing females as compared to the wild type lines. Additionally, in several mutant lines galls developed that were significantly increased in size. Moreover, the auxin exporter PIN3 in the mutant line of the three PLT genes was no longer localised at the plasma membranes of cells within the feeding site in contrast to the wild type lines. We conclude that the three PLT genes are involved in feeding site initiation and development and could potentially achieve this through regulation of PIN.

M3 - Poster

ER -