Distribution and mechanism of Type I-E CRISPR-Cas systems

R.H.J. Staals; S.J.J. Brouns

doi:10.1007/978-3-642-34657-6_6

Distribution and mechanism of Type I-E CRISPR-Cas systems

R.H.J. Staals
, S.J.J. Brouns

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

13 Citations (Scopus)

Abstract

Although the CRISPR type I system encompasses six different subtypes (I-A to I-F), only three subtypes have been studied in detail to date. This review includes an analysis of the distribution of CRISPR-Cas systems among the different bacterial and archaeal lineages, and will focus on our mechanistic understanding of the type I-E system of Escherichia coli. We will cover the overall organization of this system, starting with a detailed description of a typical type I-E gene cluster and its associated CRISPR array. In addition, we will describe recent insights on the three different stages in CRISPR-Cas type I-mediated defense: adaptation, expression and crRNA maturation, and interference. A comparison will be presented of the physical and functional characteristics of CRISPR effector complexes from the various subtypes

Original language	English
Title of host publication	CRISPR-Cas Systems
Editors	R. Barrangou, R., J. van de Oost
Place of Publication	Berlin-Heidelberg
Publisher	Springer
Pages	145-169
ISBN (Print)	9783642346569
DOIs	https://doi.org/10.1007/978-3-642-34657-6_6
Publication status	Published - 2013

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10.1007/978-3-642-34657-6_6

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abstract = "Although the CRISPR type I system encompasses six different subtypes (I-A to I-F), only three subtypes have been studied in detail to date. This review includes an analysis of the distribution of CRISPR-Cas systems among the different bacterial and archaeal lineages, and will focus on our mechanistic understanding of the type I-E system of Escherichia coli. We will cover the overall organization of this system, starting with a detailed description of a typical type I-E gene cluster and its associated CRISPR array. In addition, we will describe recent insights on the three different stages in CRISPR-Cas type I-mediated defense: adaptation, expression and crRNA maturation, and interference. A comparison will be presented of the physical and functional characteristics of CRISPR effector complexes from the various subtypes",

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AU - Staals, R.H.J.

AU - Brouns, S.J.J.

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AB - Although the CRISPR type I system encompasses six different subtypes (I-A to I-F), only three subtypes have been studied in detail to date. This review includes an analysis of the distribution of CRISPR-Cas systems among the different bacterial and archaeal lineages, and will focus on our mechanistic understanding of the type I-E system of Escherichia coli. We will cover the overall organization of this system, starting with a detailed description of a typical type I-E gene cluster and its associated CRISPR array. In addition, we will describe recent insights on the three different stages in CRISPR-Cas type I-mediated defense: adaptation, expression and crRNA maturation, and interference. A comparison will be presented of the physical and functional characteristics of CRISPR effector complexes from the various subtypes

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DO - 10.1007/978-3-642-34657-6_6

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BT - CRISPR-Cas Systems

A2 - Barrangou, R., R.

A2 - van de Oost, J.

PB - Springer

CY - Berlin-Heidelberg

ER -

Distribution and mechanism of Type I-E CRISPR-Cas systems

Abstract

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