Distribution and mechanism of Type I-E CRISPR-Cas systems

R.H.J. Staals, S.J.J. Brouns

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Citations (Scopus)


Although the CRISPR type I system encompasses six different subtypes (I-A to I-F), only three subtypes have been studied in detail to date. This review includes an analysis of the distribution of CRISPR-Cas systems among the different bacterial and archaeal lineages, and will focus on our mechanistic understanding of the type I-E system of Escherichia coli. We will cover the overall organization of this system, starting with a detailed description of a typical type I-E gene cluster and its associated CRISPR array. In addition, we will describe recent insights on the three different stages in CRISPR-Cas type I-mediated defense: adaptation, expression and crRNA maturation, and interference. A comparison will be presented of the physical and functional characteristics of CRISPR effector complexes from the various subtypes
Original languageEnglish
Title of host publicationCRISPR-Cas Systems
EditorsR. Barrangou, R., J. van de Oost
Place of PublicationBerlin-Heidelberg
PublisherSpringer Verlag
ISBN (Print)9783642346569
Publication statusPublished - 2013

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    Staals, R. H. J., & Brouns, S. J. J. (2013). Distribution and mechanism of Type I-E CRISPR-Cas systems. In R. Barrangou, R., & J. van de Oost (Eds.), CRISPR-Cas Systems (pp. 145-169). Berlin-Heidelberg: Springer Verlag. https://doi.org/10.1007/978-3-642-34657-6_6