Distinct roles for strigolactones in cyst nematode parasitism of Arabidopsis roots

Carmen M. Escudero Martinez, Nina Guarneri, Hein Overmars, Casper van Schaik, Harro Bouwmeester, Carolien Ruyter-spira, Aska Goverse

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Phytohormones play an essential role in different stages of plant-nematode interactions. Strigolactones (SLs) are a novel class of plant hormones which play an important role in plant development. Furthermore, certain soil-inhabiting organisms exploit this plant molecule as allelochemical. However, whether SLs play a role in plant parasitism by nematodes is as yet unknown. This prompted us to investigate the potential role of SLs in different stages of the nematode life cycle using the beet cyst nematode Heterodera schachtii and Arabidopsis as a model system. We analyzed the effect of SLs on cyst nematode hatching, host attraction and invasion, and the establishment of a feeding relation upon infection of the SL deficient mutant max4-1 and the SL signaling mutant max2-1. In addition, infection assays were performed under phosphate shortage to enhance SL production and in the presence of the synthetic SL analog GR24. From this study, we can conclude that SLs do not contribute to cyst nematode hatching at the levels tested but that they do play a role in host attraction and subsequent invasion in a MAX2 dependent manner. Furthermore, we observed that increased levels of exogenous and endogenous SLs change the root invasion zone. Upon root infection, cyst nematode development was enhanced in both the max2-1 and max4-1 mutants due to the formation of enlarged feeding cells. These data provide evidence for distinct roles of SLs during cyst nematode parasitism of plant roots.
Original languageEnglish
Pages (from-to)129-140
JournalEuropean Journal of Plant Pathology
Volume154
Issue number1
Early online date18 Feb 2019
DOIs
Publication statusPublished - May 2019

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cyst nematodes
parasitism
Arabidopsis
plant hormones
mutants
hatching
Nematoda
infection
Heterodera schachtii
allelochemicals
plant parasitic nematodes
beets
plant development
life cycle (organisms)
phosphates
assays
cells

Cite this

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title = "Distinct roles for strigolactones in cyst nematode parasitism of Arabidopsis roots",
abstract = "Phytohormones play an essential role in different stages of plant-nematode interactions. Strigolactones (SLs) are a novel class of plant hormones which play an important role in plant development. Furthermore, certain soil-inhabiting organisms exploit this plant molecule as allelochemical. However, whether SLs play a role in plant parasitism by nematodes is as yet unknown. This prompted us to investigate the potential role of SLs in different stages of the nematode life cycle using the beet cyst nematode Heterodera schachtii and Arabidopsis as a model system. We analyzed the effect of SLs on cyst nematode hatching, host attraction and invasion, and the establishment of a feeding relation upon infection of the SL deficient mutant max4-1 and the SL signaling mutant max2-1. In addition, infection assays were performed under phosphate shortage to enhance SL production and in the presence of the synthetic SL analog GR24. From this study, we can conclude that SLs do not contribute to cyst nematode hatching at the levels tested but that they do play a role in host attraction and subsequent invasion in a MAX2 dependent manner. Furthermore, we observed that increased levels of exogenous and endogenous SLs change the root invasion zone. Upon root infection, cyst nematode development was enhanced in both the max2-1 and max4-1 mutants due to the formation of enlarged feeding cells. These data provide evidence for distinct roles of SLs during cyst nematode parasitism of plant roots.",
author = "{Escudero Martinez}, {Carmen M.} and Nina Guarneri and Hein Overmars and {van Schaik}, Casper and Harro Bouwmeester and Carolien Ruyter-spira and Aska Goverse",
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Distinct roles for strigolactones in cyst nematode parasitism of Arabidopsis roots. / Escudero Martinez, Carmen M.; Guarneri, Nina; Overmars, Hein; van Schaik, Casper; Bouwmeester, Harro; Ruyter-spira, Carolien; Goverse, Aska.

In: European Journal of Plant Pathology, Vol. 154, No. 1, 05.2019, p. 129-140.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Escudero Martinez, Carmen M.

AU - Guarneri, Nina

AU - Overmars, Hein

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AU - Ruyter-spira, Carolien

AU - Goverse, Aska

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