Discrepancy between Species Borders at Morphological and Molecular Levels in the Genus Cochliopodium (Amoebozoa, Himatismenida), with the Description of Cochliopodium plurinucleolum n. sp.

Stefan Geisen*, Alexander Kudryavtsev, Michael Bonkowski, Alexey Smirnov

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

28 Citations (Scopus)

Abstract

Amoebae of the genus Cochliopodium are characterized by a tectum that is a layer of scales covering the dorsal surface of the cell. A combination of scale structure, morphological features and, nowadays, molecular information allows species discrimination. Here we describe a soil species Cochliopodium plurinucleolum n. sp. that besides strong genetic divergence from all currently described species of Cochliopodium differs morphologically by the presence of several peripheral nucleoli in the nucleus. Further, we unambiguously show that the Golgi attachment associated with a dictyosome in Cochliopodium is a cytoplasmic microtubule organizing center (MTOC). Last, we provide detailed morphological and molecular information on the sister clade of C. plurinucleolum, containing C. minus, C. minutoidum, C. pentatrifurcatum and C. megatetrastylus. These species share nearly identical sequences of both, small subunit ribosomal RNA and partial Cox1 genes, and nearly identical structure of the scales. Scales of C. pentatrifurcatum differ, however, strongly from scales of the others while sequences of C. pentatrifurcatum and C. minus are nearly identical. These discrepancies urge for future sampling efforts to disentangle species characteristics within Cochliopdium and to investigate morphological and molecular patterns that allow reliable species differentiation.

Original languageEnglish
Pages (from-to)364-383
JournalProtist
Volume165
Issue number3
DOIs
Publication statusPublished - May 2014
Externally publishedYes

Keywords

  • Amoebozoa
  • Cochliopodium
  • MTOC
  • Phylogeny
  • Scales
  • Ultrastructure

Fingerprint Dive into the research topics of 'Discrepancy between Species Borders at Morphological and Molecular Levels in the Genus Cochliopodium (Amoebozoa, Himatismenida), with the Description of Cochliopodium plurinucleolum n. sp.'. Together they form a unique fingerprint.

  • Cite this