Discovery of a DFR gene that controls anthocyanin accumulation in the spiny Solanum group: roles of a natural promoter variant and alternative splicing

Xing Wang, Xueping Chen*, Shuangxia Luo, Wei Ma, Na Li, Weiwei Zhang, Yury Tikunov, Shuxin Xuan, Jianjun Zhao, Yanhua Wang, Gengdi Zheng, Ping Yu, Yuling Bai, Arnaud Bovy*, Shuxing Shen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Anthocyanins are important pigments that impart color in plants. In Solanum, different species display various fruit or flower colors due to varying degrees of anthocyanin accumulation. Here we identified two anthocyanin-free mutants from an ethylmethane sulfonate-induced mutant library and naturally occurring mutants in Solanum melongena, with mutations in the 5′ splicing site of the second intron of dihydroflavonol-4-reductase (DFR) – leading to altered splicing. Further study revealed that alternative splicing of the second intron was closely related to anthocyanin accumulation in 17 accessions from three cultivated species: S. melongena, Solanum macrocarpon and Solanum aethiopicum, and their wild related species. Analysis of natural variations of DFR, using an expanded population including 282 accessions belonging to the spiny Solanum group, identified a single-nucleotide polymorphism in the MYB recognition site in the promoter region, which causes differential expression of DFR and affects anthocyanin accumulation in fruits of the detected accessions. Our study suggests that, owing to years of domestication, the natural variation in the DFR promoter region and the alternative splicing of the DFR gene account for altered anthocyanin accumulation during spiny Solanum domestication.

Original languageEnglish
Pages (from-to)1096-1109
JournalPlant Journal
Volume111
Issue number4
Early online date24 Jun 2022
DOIs
Publication statusPublished - Aug 2022

Keywords

  • alternative splicing
  • anthocyanin biosynthesis
  • dihydroflavonol-4-reductase
  • flower color
  • fruit color
  • natural variations
  • Solanum aethiopicum
  • Solanum melongena

Fingerprint

Dive into the research topics of 'Discovery of a DFR gene that controls anthocyanin accumulation in the spiny Solanum group: roles of a natural promoter variant and alternative splicing'. Together they form a unique fingerprint.

Cite this