Discovery and reconstitution of the secoiridoid pathway of Catharanthus roseus

 

Terpene indole alkaloids (TIAs) are important plant-produced secondary metabolites for

humans, because of their anti-cancer properties. The production of TIAs still fully relies on

extraction from medicinal plants like Catharanthus roseus, which only contains extreme low

amounts of these compounds and new ways need to be found to efficiently produce these

anticancer drugs at low cost. The common precursor for TIAs is strictosidine and in my PhD

project I tried to produce strictosidine in fast-growing tobacco by transferring the genes of the

whole biosynthesis pathway into tobacco. At the onset of my project 6 out of the presumed

12 genes of the pathway in C. roseus had not been discovered yet. My thesis tells the story of

discovery and characterization of the missing genes and reconstruction of the full strictosidine

pathway in tobacco.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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