Discovering novel hydrolases from hot environments

Roland Wohlgemuth, Jennifer Littlechild, Daniela Monti, Kirk Schnorr, Teunke van Rossum, Bettina Siebers, Peter Menzel, Ilya V. Kublanov, Anne Gunn Rike, Georgios Skretas, Zalan Szabo, Xu Peng, Mark J. Young

Research output: Contribution to journalReview articleAcademicpeer-review

3 Citations (Scopus)

Abstract

Novel hydrolases from hot and other extreme environments showing appropriate performance and/or novel functionalities and new approaches for their systematic screening are of great interest for developing new processes, for improving safety, health and environment issues. Existing processes could benefit as well from their properties. The workflow, based on the HotZyme project, describes a multitude of technologies and their integration from discovery to application, providing new tools for discovering, identifying and characterizing more novel thermostable hydrolases with desired functions from hot terrestrial and marine environments. To this end, hot springs worldwide were mined, resulting in hundreds of environmental samples and thousands of enrichment cultures growing on polymeric substrates of industrial interest. Using high-throughput sequencing and bioinformatics, 15 hot spring metagenomes, as well as several sequenced isolate genomes and transcriptomes were obtained. To facilitate the discovery of novel hydrolases, the annotation platform Anastasia and a whole-cell bioreporter-based functional screening method were developed. Sequence-based screening and functional screening together resulted in about 100 potentially new hydrolases of which more than a dozen have been characterized comprehensively from a biochemical and structural perspective. The characterized hydrolases include thermostable carboxylesterases, enol lactonases, quorum sensing lactonases, gluconolactonases, epoxide hydrolases, and cellulases. Apart from these novel thermostable hydrolases, the project generated an enormous amount of samples and data, thereby allowing the future discovery of even more novel enzymes.

LanguageEnglish
Pages2077-2100
JournalBiotechnology Advances
Volume36
Issue number8
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Hydrolases
Screening
Hot springs
Hot Springs
gluconolactonase
Carboxylic Ester Hydrolases
Metagenome
Epoxide Hydrolases
Cellulases
Quorum Sensing
Workflow
Bioinformatics
Computational Biology
Transcriptome
Genes
Throughput
Health
Genome
Technology
Enzymes

Keywords

  • Biocatalysis
  • Enrichment
  • Enzyme characterization
  • Enzyme screening
  • Gene expression
  • Genomics
  • Hydrolases
  • Metagenomics
  • Sequencing
  • Thermophiles

Cite this

Wohlgemuth, R., Littlechild, J., Monti, D., Schnorr, K., van Rossum, T., Siebers, B., ... Young, M. J. (2018). Discovering novel hydrolases from hot environments. Biotechnology Advances, 36(8), 2077-2100. https://doi.org/10.1016/j.biotechadv.2018.09.004
Wohlgemuth, Roland ; Littlechild, Jennifer ; Monti, Daniela ; Schnorr, Kirk ; van Rossum, Teunke ; Siebers, Bettina ; Menzel, Peter ; Kublanov, Ilya V. ; Rike, Anne Gunn ; Skretas, Georgios ; Szabo, Zalan ; Peng, Xu ; Young, Mark J. / Discovering novel hydrolases from hot environments. In: Biotechnology Advances. 2018 ; Vol. 36, No. 8. pp. 2077-2100.
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Wohlgemuth, R, Littlechild, J, Monti, D, Schnorr, K, van Rossum, T, Siebers, B, Menzel, P, Kublanov, IV, Rike, AG, Skretas, G, Szabo, Z, Peng, X & Young, MJ 2018, 'Discovering novel hydrolases from hot environments', Biotechnology Advances, vol. 36, no. 8, pp. 2077-2100. https://doi.org/10.1016/j.biotechadv.2018.09.004

Discovering novel hydrolases from hot environments. / Wohlgemuth, Roland; Littlechild, Jennifer; Monti, Daniela; Schnorr, Kirk; van Rossum, Teunke; Siebers, Bettina; Menzel, Peter; Kublanov, Ilya V.; Rike, Anne Gunn; Skretas, Georgios; Szabo, Zalan; Peng, Xu; Young, Mark J.

In: Biotechnology Advances, Vol. 36, No. 8, 01.12.2018, p. 2077-2100.

Research output: Contribution to journalReview articleAcademicpeer-review

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T1 - Discovering novel hydrolases from hot environments

AU - Wohlgemuth, Roland

AU - Littlechild, Jennifer

AU - Monti, Daniela

AU - Schnorr, Kirk

AU - van Rossum, Teunke

AU - Siebers, Bettina

AU - Menzel, Peter

AU - Kublanov, Ilya V.

AU - Rike, Anne Gunn

AU - Skretas, Georgios

AU - Szabo, Zalan

AU - Peng, Xu

AU - Young, Mark J.

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N2 - Novel hydrolases from hot and other extreme environments showing appropriate performance and/or novel functionalities and new approaches for their systematic screening are of great interest for developing new processes, for improving safety, health and environment issues. Existing processes could benefit as well from their properties. The workflow, based on the HotZyme project, describes a multitude of technologies and their integration from discovery to application, providing new tools for discovering, identifying and characterizing more novel thermostable hydrolases with desired functions from hot terrestrial and marine environments. To this end, hot springs worldwide were mined, resulting in hundreds of environmental samples and thousands of enrichment cultures growing on polymeric substrates of industrial interest. Using high-throughput sequencing and bioinformatics, 15 hot spring metagenomes, as well as several sequenced isolate genomes and transcriptomes were obtained. To facilitate the discovery of novel hydrolases, the annotation platform Anastasia and a whole-cell bioreporter-based functional screening method were developed. Sequence-based screening and functional screening together resulted in about 100 potentially new hydrolases of which more than a dozen have been characterized comprehensively from a biochemical and structural perspective. The characterized hydrolases include thermostable carboxylesterases, enol lactonases, quorum sensing lactonases, gluconolactonases, epoxide hydrolases, and cellulases. Apart from these novel thermostable hydrolases, the project generated an enormous amount of samples and data, thereby allowing the future discovery of even more novel enzymes.

AB - Novel hydrolases from hot and other extreme environments showing appropriate performance and/or novel functionalities and new approaches for their systematic screening are of great interest for developing new processes, for improving safety, health and environment issues. Existing processes could benefit as well from their properties. The workflow, based on the HotZyme project, describes a multitude of technologies and their integration from discovery to application, providing new tools for discovering, identifying and characterizing more novel thermostable hydrolases with desired functions from hot terrestrial and marine environments. To this end, hot springs worldwide were mined, resulting in hundreds of environmental samples and thousands of enrichment cultures growing on polymeric substrates of industrial interest. Using high-throughput sequencing and bioinformatics, 15 hot spring metagenomes, as well as several sequenced isolate genomes and transcriptomes were obtained. To facilitate the discovery of novel hydrolases, the annotation platform Anastasia and a whole-cell bioreporter-based functional screening method were developed. Sequence-based screening and functional screening together resulted in about 100 potentially new hydrolases of which more than a dozen have been characterized comprehensively from a biochemical and structural perspective. The characterized hydrolases include thermostable carboxylesterases, enol lactonases, quorum sensing lactonases, gluconolactonases, epoxide hydrolases, and cellulases. Apart from these novel thermostable hydrolases, the project generated an enormous amount of samples and data, thereby allowing the future discovery of even more novel enzymes.

KW - Biocatalysis

KW - Enrichment

KW - Enzyme characterization

KW - Enzyme screening

KW - Gene expression

KW - Genomics

KW - Hydrolases

KW - Metagenomics

KW - Sequencing

KW - Thermophiles

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DO - 10.1016/j.biotechadv.2018.09.004

M3 - Review article

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Wohlgemuth R, Littlechild J, Monti D, Schnorr K, van Rossum T, Siebers B et al. Discovering novel hydrolases from hot environments. Biotechnology Advances. 2018 Dec 1;36(8):2077-2100. https://doi.org/10.1016/j.biotechadv.2018.09.004