Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

Jochem N.A. Vink; Koen J.A. Martens; Marnix Vlot; Rebecca E. McKenzie; Cristóbal Almendros; Boris Estrada Bonilla; Daan J.W. Brocken; Johannes Hohlbein; Stan J.J. Brouns

doi:10.1016/j.molcel.2019.10.021

Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

Jochem N.A. Vink, Koen J.A. Martens, Marnix Vlot, Rebecca E. McKenzie, Cristóbal Almendros, Boris Estrada Bonilla, Daan J.W. Brocken, Johannes Hohlbein^*, Stan J.J. Brouns

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

1 Citation (Scopus)

Abstract

Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic elements from the cell.

Original language	English
Pages (from-to)	39-50.e10
Journal	Molecular Cell
Volume	77
Issue number	1
DOIs	https://doi.org/10.1016/j.molcel.2019.10.021
Publication status	Published - 2 Jan 2020

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Keywords

arms race
Cascade
CRISPR-Cas
PALM
PAM
photo-activation localization microscopy
single molecule
single-particle tracking
target search

Cite this

Vink, J. N. A., Martens, K. J. A., Vlot, M., McKenzie, R. E., Almendros, C., Estrada Bonilla, B., ... Brouns, S. J. J. (2020). Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism. Molecular Cell, 77(1), 39-50.e10. https://doi.org/10.1016/j.molcel.2019.10.021

@article{8168469a46b94ac5b11f112d9653644b,

title = "Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism",

abstract = "Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic elements from the cell.",

keywords = "arms race, Cascade, CRISPR-Cas, PALM, PAM, photo-activation localization microscopy, single molecule, single-particle tracking, target search",

author = "Vink, {Jochem N.A.} and Martens, {Koen J.A.} and Marnix Vlot and McKenzie, {Rebecca E.} and Crist{\'o}bal Almendros and {Estrada Bonilla}, Boris and Brocken, {Daan J.W.} and Johannes Hohlbein and Brouns, {Stan J.J.}",

year = "2020",

month = "1",

day = "2",

doi = "10.1016/j.molcel.2019.10.021",

language = "English",

volume = "77",

pages = "39--50.e10",

journal = "Molecular Cell",

issn = "1097-2765",

publisher = "Elsevier",

number = "1",

}

Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism. / Vink, Jochem N.A.; Martens, Koen J.A.; Vlot, Marnix; McKenzie, Rebecca E.; Almendros, Cristóbal; Estrada Bonilla, Boris; Brocken, Daan J.W.; Hohlbein, Johannes; Brouns, Stan J.J.

In: Molecular Cell, Vol. 77, No. 1, 02.01.2020, p. 39-50.e10.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

AU - Vink, Jochem N.A.

AU - Martens, Koen J.A.

AU - Vlot, Marnix

AU - McKenzie, Rebecca E.

AU - Almendros, Cristóbal

AU - Estrada Bonilla, Boris

AU - Brocken, Daan J.W.

AU - Hohlbein, Johannes

AU - Brouns, Stan J.J.

PY - 2020/1/2

Y1 - 2020/1/2

N2 - Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic elements from the cell.

AB - Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic elements from the cell.

KW - arms race

KW - Cascade

KW - CRISPR-Cas

KW - PALM

KW - PAM

KW - photo-activation localization microscopy

KW - single molecule

KW - single-particle tracking

KW - target search

U2 - 10.1016/j.molcel.2019.10.021

DO - 10.1016/j.molcel.2019.10.021

M3 - Article

VL - 77

SP - 39-50.e10

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 1

ER -

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10.1016/j.molcel.2019.10.021

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Adaptive immunity in prokaryotes: how Bacteria do not forgive and do not forget their enemies

Project: EU research project

Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

Abstract

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Keywords

Cite this

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Projects

Adaptive immunity in prokaryotes: how Bacteria do not forgive and do not forget their enemies