Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

Jochem N.A. Vink, Koen J.A. Martens, Marnix Vlot, Rebecca E. McKenzie, Cristóbal Almendros, Boris Estrada Bonilla, Daan J.W. Brocken, Johannes Hohlbein*, Stan J.J. Brouns

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic elements from the cell.

Original languageEnglish
Pages (from-to)39-50.e10
JournalMolecular Cell
Volume77
Issue number1
DOIs
Publication statusPublished - 2 Jan 2020

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Keywords

  • arms race
  • Cascade
  • CRISPR-Cas
  • PALM
  • PAM
  • photo-activation localization microscopy
  • single molecule
  • single-particle tracking
  • target search

Cite this

Vink, J. N. A., Martens, K. J. A., Vlot, M., McKenzie, R. E., Almendros, C., Estrada Bonilla, B., ... Brouns, S. J. J. (2020). Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism. Molecular Cell, 77(1), 39-50.e10. https://doi.org/10.1016/j.molcel.2019.10.021