Direct profiling of environmental microbial populations by thermal dissociation analysis of native rRNAs hybridized to oligonucleotide microarrays

S. El Fantroussi, H. Urakawa, A.E. Bernhard, J.J. Kelly, P.A. Noble, H. Smidt, G.M. Yershov, D.A. Stahl

Research output: Contribution to journalArticleAcademicpeer-review

95 Citations (Scopus)

Abstract

Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences
Original languageEnglish
Pages (from-to)2377-2382
JournalApplied and Environmental Microbiology
Volume69
Issue number4
DOIs
Publication statusPublished - 2003

Keywords

  • 16s ribosomal-rna
  • gradient gel-electrophoresis
  • oligodeoxynucleotide probes
  • genes
  • pcr
  • identification
  • bacteria
  • bacterioplankton
  • diversity
  • database

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