Direct creation of biopatterns via a combination of laser-based techniques and click chemistry

Marianneza Chatzipetrou, Maria Massaouti, George Tsekenis, Anke K. Trilling, Esther van Andel, Luc Scheres, Maarten M.J. Smulders, Han Zuilhof*, Ioanna Zergioti

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

In this paper, we present the immobilization of thiol-modified aptamers on alkyne- or alkene-terminated silicon nitride surfaces by laser-induced click chemistry reactions. The aptamers are printed onto the surface by laser-induced forward transfer (LIFT), which also induces the covalent bonding of the aptamers by thiol-ene or thiol-yne reactions that occur upon UV irradiation of the thiol-modified aptamers with ns laser pulses. This combination of LIFT and laser-induced click chemistry allows the creation of high-resolution patterns without the need for masks. Whereas the click chemistry already takes place during the printing process (single-step process) by the laser pulse used for the printing process, further irradiation of the LIFT-printed aptamers by laser pulses (two-step process) leads to a further increase in the immobilization efficiency. (Figure Presented).

Original languageEnglish
Pages (from-to)848-853
JournalLangmuir
Volume33
Issue number4
DOIs
Publication statusPublished - 2017

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