Direct comparative analysis of conventional and directional freezing for the cryopreservation of whole ovaries

S. Maffei, M. Hanenberg, G. Pennarossa, J. Roberto V. Silva, A. Tiziana, L. Brevini, D. Pharm, A. Arav, F. Gandolfi

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12 Citations (Scopus)

Abstract

INTERVENTION(S): Eighty-one ovaries were randomly assigned to fresh control, conventional freezing (CF), and directional freezing (DF) group. Ovaries of CF and DF groups were perfused via the ovarian artery with Leibovitz L-15 medium, 10% fetal bovine serum, and 1.5 M dimethyl sulfoxide for 5 minutes. Each ovary was inserted into a glass test tube containing 10 mL of the same solution and cooled to -100°C or -70°C, respectively. Ovaries were stored in liquid nitrogen for a minimum of 2 weeks. MAIN OUTCOME MEASURE(S): Structural integrity of cortical and medulla regions, vascular integrity, follicle in vitro development, cell proliferation, and DNA damage and repair. RESULT(S): All examined parameters indicate that the structure of DF ovaries remains largely intact and comparable to fresh controls, whereas significant damages were observed in CF ovaries. CONCLUSION(S): Directional freezing allows good preservation of whole ovaries, with most of the parameters taken into consideration almost identical to those recorded in fresh control samples. This encourages a reconsideration of the possible use of whole-ovary cryopreservation as a viable alternative to cortical fragments.
Original languageEnglish
Pages (from-to)1122-1131
JournalFertility and Sterility
Volume100
Issue number4
DOIs
Publication statusPublished - 2013

Keywords

  • fertility preservation
  • sheep ovaries
  • in-vitro
  • heterotopic autotransplantation
  • microvascular anastomosis
  • primordial follicles
  • vascular pedicle
  • ovine ovaries
  • intact ovary
  • live birth

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    Maffei, S., Hanenberg, M., Pennarossa, G., Roberto V. Silva, J., Tiziana, A., Brevini, L., ... Gandolfi, F. (2013). Direct comparative analysis of conventional and directional freezing for the cryopreservation of whole ovaries. Fertility and Sterility, 100(4), 1122-1131. https://doi.org/10.1016/j.fertnstert.2013.06.003