Direct biosensor immunoassays for the detection of nonmilk proteins in milk powder

W. Haasnoot, K. Olieman, G. Cazemier, R. Verheijen

Research output: Contribution to journalArticleAcademicpeer-review

58 Citations (Scopus)


The low prices of some nonmilk proteins make them attractive as potential adulterants in dairy products. An optical biosensor (BIACORE 3000) was used to develop a direct and combined biosensor immunoassay (BIA) for the simultaneous detection of soy, pea, and soluble wheat proteins in milk powders. Affinity-purified polyclonal antibodies raised against the three protein sources were immobilized in different flow channels (Fcs) on the biosensor chip (CM5). Dissolved milk powders were injected (20 L injections at 20 L min-1) through the serially connected Fcs, and the antibody-bound plant proteins were detected directly. The total run time between samples, including a regeneration step with 5 L of 10 mM HCl, was 5 min. The limits of detection in milk powder were below 0.1␘f plant protein in the total milk protein content. The antibodies also recognized some proteins from other plant sources, which made this BIA even more suitable as a broad screening assay for nonmilk proteins.
Original languageEnglish
Pages (from-to)5201-5206
JournalJournal of Agricultural and Food Chemistry
Issue number11
Publication statusPublished - 2001


  • plant proteins
  • soy
  • pea
  • soluble wheat proteins


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