Diploid apomicts of the Boechera holboelli complex display large scale chromosome substitutions and aberrant chromosomes

L. Kantama, T.F. Sharbel, M.E. Schranz, T. Mitchell-Olds, S.C. de Vries, J.H.S.G.M. de Jong

Research output: Contribution to journalArticleAcademicpeer-review

90 Citations (Scopus)

Abstract

We conducted a cytogenetic study of sexual lines of Boechera stricta and Boechera holboellii (2n = 14) and seven diploid apomictic accessions of their interspecific hybrid Boechera divaricarpa and B. holboellii (2n = 14 or 15). By studying chromosome morphology, rDNA repeats, genome painting, male meiosis, pollen morphology, and flow-cytometry seed screens, we revealed an unexpected plethora of chromosome forms, pairing behavior, and hybrid composition in all apomictic lines. Genome painting demonstrated that the apomicts are alloploid with variable numbers of B. stricta and B. holboellii-like chromosomes. We assume that large-scale homeologous chromosome substitutions took place in the apomictic hybrids that resulted from recurrent diploid-polyploid transitions through restitutional meiosis and polyploicly-diploid transitions through reductional meiosis. A second peculiarity was the presence of a largely heterochromatic chromosome (Het) in all apomictic accessions (2n = 14 and 15) and an additional smaller chromosome (Den in the aneuploids (2n = 15). Both chromosomes share repetitive pericentromere repeats with those from the sexual B. stricta, suggesting that they originated from this species. Pairing and behavior at meiosis I of the Het share features with both Y and B chromosomes and suggest that the Del arose from a translocation event or homeologous recombination between a B. holboellii (or related taxon) and a B. stricta chromosome. Based on its presence exclusively in apomictic accessions, we propose that the Het chromosome plays a role in the genetic control of apomixis.
Original languageEnglish
Pages (from-to)14026-14031
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
DOIs
Publication statusPublished - 2007

Keywords

  • in-situ hybridization
  • gene-expression
  • pennisetum-squamulatum
  • cenchrus-ciliaris
  • arabis-drummondii
  • b-chromosome
  • brassicaceae
  • evolution
  • arabidopsis
  • apomixis

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