Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution.

C.E. Klostermann, P.L. Buwalda, H. Leemhuis, P. de Vos, H.A. Schols, J.H. Bitter*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14–76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A >>> B), followed by molecular weight (Mw) (high DP >> low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.

Original languageEnglish
Article number118069
JournalCarbohydrate Polymers
Volume265
DOIs
Publication statusPublished - 1 Aug 2021

Keywords

  • Dietary fiber
  • HPSEC
  • Prebiotics
  • Resistant starch type 3
  • α-glucan

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