Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution.

C.E. Klostermann; P.L. Buwalda; H. Leemhuis; P. de Vos; H.A. Schols; J.H. Bitter

doi:10.1016/j.carbpol.2021.118069

Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution.

C.E. Klostermann, P.L. Buwalda, H. Leemhuis, P. de Vos, H.A. Schols, J.H. Bitter^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

35 Citations (Scopus)

Abstract

Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14–76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A >>> B), followed by molecular weight (Mw) (high DP >> low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.

Original language	English
Article number	118069
Journal	Carbohydrate Polymers
Volume	265
DOIs	https://doi.org/10.1016/j.carbpol.2021.118069
Publication status	Published - 1 Aug 2021

Keywords

Dietary fiber
HPSEC
Prebiotics
Resistant starch type 3
α-glucan

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title = "Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution.",

abstract = "Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14–76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A >>> B), followed by molecular weight (Mw) (high DP >> low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.",

keywords = "Dietary fiber, HPSEC, Prebiotics, Resistant starch type 3, α-glucan",

author = "C.E. Klostermann and P.L. Buwalda and H. Leemhuis and {de Vos}, P. and H.A. Schols and J.H. Bitter",

year = "2021",

month = aug,

day = "1",

doi = "10.1016/j.carbpol.2021.118069",

language = "English",

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journal = "Carbohydrate Polymers",

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TY - JOUR

T1 - Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution.

AU - Klostermann, C.E.

AU - Buwalda, P.L.

AU - Leemhuis, H.

AU - de Vos, P.

AU - Schols, H.A.

AU - Bitter, J.H.

PY - 2021/8/1

Y1 - 2021/8/1

N2 - Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14–76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A >>> B), followed by molecular weight (Mw) (high DP >> low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.

AB - Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14–76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A >>> B), followed by molecular weight (Mw) (high DP >> low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.

KW - Dietary fiber

KW - HPSEC

KW - Prebiotics

KW - Resistant starch type 3

KW - α-glucan

U2 - 10.1016/j.carbpol.2021.118069

DO - 10.1016/j.carbpol.2021.118069

M3 - Article

AN - SCOPUS:85104103669

SN - 0144-8617

VL - 265

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

M1 - 118069

ER -

Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution.

Abstract

Keywords

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