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Abstract
Certain mycotoxins may be present in plant materials as their glucosides. The question is whether these glucosides may be hydrolysed into their parent compounds in the gastro-intestinal tract (GI-tract), thus increasing the exposure. Therefore, the potential hydrolysis of deoxynivalenol-3-ß-glucoside (DON-3G) to deoxynivalenol (DON) was assessed in two in vitro models representing the human upper GI-tract (mouth, stomach and small intestine). In a fed digestion model, there was no evidence of release of DON from DON-3G, spiked at a level of 2,778 µg DON- 3G/kg food. This shows that the conditions in the GI-tract do not result in hydrolysis of this glucoside into the original mycotoxin. The absorption and transformation of DON-3G in the small intestine was assessed in an in vitro model with human Caco-2 cells in a Transwell system. No evidence was found for the transformation of DON-3G to DON by the Caco-2 cells in both the apical or basolateral side in 24 hours (cells were exposed to 2.4 nmol DON- 3G/ml medium). However, when DON itself was added to the apical side an amount of 23% of the spiked DON was detected in the basolateral side after 24 hours (cells were exposed to 2.3 nmol/ml medium). In conclusion, no evidence was found in the in vitro experiments for significant elevated exposure of humans to DON, since DON- 3G was not hydrolysed to DON in the digestion model representing the upper part of the GI-tract and DON-3G was not hydrolysed to DON by the intestinal epithelial Caco-2 cells. It was shown that bioavailability of DON-3G in humans may be low as compared to DON since Caco-2 cells did not absorb DON-3G, in contrast to DON.
Original language | English |
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Pages (from-to) | 319-324 |
Journal | World Mycotoxin Journal |
Volume | 5 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2012 |
Keywords
- mass-spectrometry
- digestion model
- deoxynivalenol
- wheat
- bioaccessibility
- mycotoxins
- caco-2
- food
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Dive into the research topics of 'Digestibility and absorption of deoxynivalenol-3-ß-glucoside in in vitro models'. Together they form a unique fingerprint.Projects
- 1 Finished
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Methode-ontwikkeling en surveys plantentoxinen t.b.v. WOT (WOT-02-001-018, WOT-02-001-052)
de Nijs, M. (Project Leader)
1/01/10 → 31/12/21
Project: LVVN project