Diffusible repression of cytokinin signalling produces endodermal symmetry and passage cells

Tonni Grube Andersen*, Sadaf Naseer, Robertas Ursache, Brecht Wybouw, Wouter Smet, Bert De Rybel, Joop E.M. Vermeer, Niko Geldner

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)

Abstract

In vascular plants, the root endodermis surrounds the central vasculature as a protective sheath that is analogous to the polarized epithelium in animals, and contains ring-shaped Casparian strips that restrict diffusion. After an initial lag phase, individual endodermal cells suberize in an apparently random fashion to produce 'patchy' suberization that eventually generates a zone of continuous suberin deposition. Casparian strips and suberin lamellae affect paracellular and transcellular transport, respectively. Most angiosperms maintain some isolated cells in an unsuberized state as so-called 'passage cells', which have previously been suggested to enable uptake across an otherwise-impermeable endodermal barrier. Here we demonstrate that these passage cells are late emanations of a meristematic patterning process that reads out the underlying non-radial symmetry of the vasculature. This process is mediated by the non-cell-autonomous repression of cytokinin signalling in the root meristem, and leads to distinct phloem- and xylem-pole-associated endodermal cells. The latter cells can resist abscisic acid-dependent suberization to produce passage cells. Our data further demonstrate that, during meristematic patterning, xylem-pole-associated endodermal cells can dynamically alter passage-cell numbers in response to nutrient status, and that passage cells express transporters and locally affect the expression of transporters in adjacent cortical cells.
Original languageEnglish
Pages (from-to)529-533
JournalNature
Volume555
Issue number7697
DOIs
Publication statusPublished - 22 Mar 2018

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Cytokinins
Xylem
Transcytosis
Phloem
Angiosperms
Abscisic Acid
Plant Roots
Meristem
Blood Vessels
Epithelium
Cell Count

Cite this

Andersen, T. G., Naseer, S., Ursache, R., Wybouw, B., Smet, W., De Rybel, B., ... Geldner, N. (2018). Diffusible repression of cytokinin signalling produces endodermal symmetry and passage cells. Nature, 555(7697), 529-533. https://doi.org/10.1038/nature25976
Andersen, Tonni Grube ; Naseer, Sadaf ; Ursache, Robertas ; Wybouw, Brecht ; Smet, Wouter ; De Rybel, Bert ; Vermeer, Joop E.M. ; Geldner, Niko. / Diffusible repression of cytokinin signalling produces endodermal symmetry and passage cells. In: Nature. 2018 ; Vol. 555, No. 7697. pp. 529-533.
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Andersen, TG, Naseer, S, Ursache, R, Wybouw, B, Smet, W, De Rybel, B, Vermeer, JEM & Geldner, N 2018, 'Diffusible repression of cytokinin signalling produces endodermal symmetry and passage cells', Nature, vol. 555, no. 7697, pp. 529-533. https://doi.org/10.1038/nature25976

Diffusible repression of cytokinin signalling produces endodermal symmetry and passage cells. / Andersen, Tonni Grube; Naseer, Sadaf; Ursache, Robertas; Wybouw, Brecht; Smet, Wouter; De Rybel, Bert; Vermeer, Joop E.M.; Geldner, Niko.

In: Nature, Vol. 555, No. 7697, 22.03.2018, p. 529-533.

Research output: Contribution to journalArticleAcademicpeer-review

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Andersen TG, Naseer S, Ursache R, Wybouw B, Smet W, De Rybel B et al. Diffusible repression of cytokinin signalling produces endodermal symmetry and passage cells. Nature. 2018 Mar 22;555(7697):529-533. https://doi.org/10.1038/nature25976