Differentiation of osteoblasts on pectin-coated titanium

H. Kokkonen, C. Cassinelli, R. Verhoef, M. Morra, H.A. Schols, J. Tuukkanen

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44 Citations (Scopus)

Abstract

The gold standard for implant metals is titanium, and coatings such as collagen-I, RGD-peptide, chondroitin sulfate, and calcium phosphate have been used to modify its biocompatibility. We investigated how titanium coated with pectins, adaptable bioactive plant polysaccharides with anti-inflammatory effects, supports osteoblast differentiation. MC3T3-E1 cells, primary murine osteoblasts, and human mesenchymal cells (hMC) were cultured on titanium coated with rhamnogalacturonan-rich modified hairy regions (MHR-A and MHR-B) of apple pectin. Alkaline phosphatase (ALP) expression and activity, calcium deposition, and cell spreading were investigated. MHR-B, but not MHR-A, supports osteoblast differentiation. The MHR-A surface was not mineralized, but on MHR-B, the average mineralized area was 14.0% with MC3T3-E1 cells and 26.6% with primary osteoblasts. The ALP activity of hMCs on MHR-A was 58.3% at day 7 and 9.3% from that of MHR-B at day 10. These data indicate that modified pectin nanocoatings may enhance the biocompatibility of bone and dental implants.
Original languageEnglish
Pages (from-to)2369-2376
JournalBiomacromolecules
Volume9
Issue number9
DOIs
Publication statusPublished - 2008

Keywords

  • in-vitro
  • hairy regions
  • extracellular-matrix
  • protein adsorption
  • gene-expression
  • mc3t3-e1 cells
  • stromal cells
  • mineralization
  • proliferation
  • attachment

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