Differential induction of electrophile-responsive element-regulated genes by n-3 and n-6 polyunsaturated fatty acids

V.A. van Beelen, M.G.M. Aarts, A. Reus, H. Mooibroek, L. Sijtsma, H.J. Bosch, I.M.C.M. Rietjens, G.M. Alink

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16 Citations (Scopus)

Abstract

In this study the n-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid and docosahexaenoic acid appear to be effective inducers of electrophile-responsive element (EpRE) regulated genes, whereas the n-6 PUFA arachidonic acid is not. These n-3 PUFAs need to be oxidized to induce EpRE-regulated gene expression, as the antioxidant vitamin E can partially inhibit the PUFA induced dose-dependent effect. Results were obtained using a reporter gene assay, real-time RT-PCR and enzyme activity assays. The induction of EpRE-regulated phase II genes by n-3 PUFAs may be a major pathway by which n-3 PUFAs, in contrast to n-6 PUFAs, are chemopreventive and anticarcinogenic.
Original languageEnglish
Pages (from-to)4587-4590
JournalFEBS Letters
Volume580
Issue number19
DOIs
Publication statusPublished - 2006

Keywords

  • antioxidant response
  • fish-oil
  • dietary
  • carcinogenesis
  • mechanisms
  • protection
  • oxidation
  • reductase
  • pathway
  • cancer

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