Differential induction of electrophile-responsive element-regulated genes by n-3 and n-6 polyunsaturated fatty acids

V.A. van Beelen; M.G.M. Aarts; A. Reus; H. Mooibroek; L. Sijtsma; H.J. Bosch; I.M.C.M. Rietjens; G.M. Alink

doi:10.1016/j.febslet.2006.07.028

Differential induction of electrophile-responsive element-regulated genes by n-3 and n-6 polyunsaturated fatty acids

V.A. van Beelen, M.G.M. Aarts, A. Reus, H. Mooibroek, L. Sijtsma, H.J. Bosch, I.M.C.M. Rietjens, G.M. Alink

Research output: Contribution to journal › Article › Academic › peer-review

23 Citations (Scopus)

Abstract

In this study the n-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid and docosahexaenoic acid appear to be effective inducers of electrophile-responsive element (EpRE) regulated genes, whereas the n-6 PUFA arachidonic acid is not. These n-3 PUFAs need to be oxidized to induce EpRE-regulated gene expression, as the antioxidant vitamin E can partially inhibit the PUFA induced dose-dependent effect. Results were obtained using a reporter gene assay, real-time RT-PCR and enzyme activity assays. The induction of EpRE-regulated phase II genes by n-3 PUFAs may be a major pathway by which n-3 PUFAs, in contrast to n-6 PUFAs, are chemopreventive and anticarcinogenic.

Original language	English
Pages (from-to)	4587-4590
Journal	FEBS Letters
Volume	580
Issue number	19
DOIs	https://doi.org/10.1016/j.febslet.2006.07.028
Publication status	Published - 2006

Keywords

antioxidant response
fish-oil
dietary
carcinogenesis
mechanisms
protection
oxidation
reductase
pathway
cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{6e842ab2027a4f919a29e049e81dd912,

title = "Differential induction of electrophile-responsive element-regulated genes by n-3 and n-6 polyunsaturated fatty acids",

abstract = "In this study the n-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid and docosahexaenoic acid appear to be effective inducers of electrophile-responsive element (EpRE) regulated genes, whereas the n-6 PUFA arachidonic acid is not. These n-3 PUFAs need to be oxidized to induce EpRE-regulated gene expression, as the antioxidant vitamin E can partially inhibit the PUFA induced dose-dependent effect. Results were obtained using a reporter gene assay, real-time RT-PCR and enzyme activity assays. The induction of EpRE-regulated phase II genes by n-3 PUFAs may be a major pathway by which n-3 PUFAs, in contrast to n-6 PUFAs, are chemopreventive and anticarcinogenic.",

keywords = "antioxidant response, fish-oil, dietary, carcinogenesis, mechanisms, protection, oxidation, reductase, pathway, cancer",

author = "{van Beelen}, V.A. and M.G.M. Aarts and A. Reus and H. Mooibroek and L. Sijtsma and H.J. Bosch and I.M.C.M. Rietjens and G.M. Alink",

year = "2006",

doi = "10.1016/j.febslet.2006.07.028",

language = "English",

volume = "580",

pages = "4587--4590",

journal = "FEBS Letters",

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publisher = "Wiley",

number = "19",

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TY - JOUR

T1 - Differential induction of electrophile-responsive element-regulated genes by n-3 and n-6 polyunsaturated fatty acids

AU - van Beelen, V.A.

AU - Aarts, M.G.M.

AU - Reus, A.

AU - Mooibroek, H.

AU - Sijtsma, L.

AU - Bosch, H.J.

AU - Rietjens, I.M.C.M.

AU - Alink, G.M.

PY - 2006

Y1 - 2006

N2 - In this study the n-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid and docosahexaenoic acid appear to be effective inducers of electrophile-responsive element (EpRE) regulated genes, whereas the n-6 PUFA arachidonic acid is not. These n-3 PUFAs need to be oxidized to induce EpRE-regulated gene expression, as the antioxidant vitamin E can partially inhibit the PUFA induced dose-dependent effect. Results were obtained using a reporter gene assay, real-time RT-PCR and enzyme activity assays. The induction of EpRE-regulated phase II genes by n-3 PUFAs may be a major pathway by which n-3 PUFAs, in contrast to n-6 PUFAs, are chemopreventive and anticarcinogenic.

AB - In this study the n-3 polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid and docosahexaenoic acid appear to be effective inducers of electrophile-responsive element (EpRE) regulated genes, whereas the n-6 PUFA arachidonic acid is not. These n-3 PUFAs need to be oxidized to induce EpRE-regulated gene expression, as the antioxidant vitamin E can partially inhibit the PUFA induced dose-dependent effect. Results were obtained using a reporter gene assay, real-time RT-PCR and enzyme activity assays. The induction of EpRE-regulated phase II genes by n-3 PUFAs may be a major pathway by which n-3 PUFAs, in contrast to n-6 PUFAs, are chemopreventive and anticarcinogenic.

KW - antioxidant response

KW - fish-oil

KW - dietary

KW - carcinogenesis

KW - mechanisms

KW - protection

KW - oxidation

KW - reductase

KW - pathway

KW - cancer

U2 - 10.1016/j.febslet.2006.07.028

DO - 10.1016/j.febslet.2006.07.028

M3 - Article

SN - 0014-5793

VL - 580

SP - 4587

EP - 4590

JO - FEBS Letters

JF - FEBS Letters

IS - 19

ER -

Differential induction of electrophile-responsive element-regulated genes by n-3 and n-6 polyunsaturated fatty acids

Abstract

Keywords

UN SDGs

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