Differential gene expression is not required for facultative sex allocation: A transcriptome analysis of brain tissue in the parasitoid wasp nasonia vitripennis

Nicola Cook*, Rebecca A. Boulton, Jade Green, Urmi Trivedi, Eran Tauber, Bart A. Pannebakker, Michael G. Ritchie, David M. Shuker

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Whole-transcriptome technologies have been widely used in behavioural genetics to identify genes associated with the performance of a behaviour and provide clues to its mechanistic basis. Here, we consider the genetic basis of sex allocation behaviour in the parasitoid wasp Nasonia vitripennis. Female Nasonia facultatively vary their offspring sex ratio in line with Hamilton’s theory of local mate competition (LMC). A single female or ‘foundress’ laying eggs on a patch will lay just enough sons to fertilize her daughters. As the number of ‘foundresses’ laying eggs on a patch increases (and LMC declines), females produce increasingly male-biased sex ratios. Phenotypic studies have revealed the cues females use to estimate the level of LMC their sons will experience, but our understanding of the genetics underlying sex allocation is limited. Here, we exposed females to three foundress number conditions, i.e. three LMC conditions, and allowed them to oviposit. mRNA was extracted from only the heads of these females to target the brain tissue. The subsequent RNA-seq experiment confirmed that differential gene expression is not
Original languageEnglish
Article number171718
Number of pages8
JournalRoyal Society Open Science
Volume5
Issue number2
DOIs
Publication statusPublished - 21 Feb 2018

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Keywords

  • Behavioural genetics
  • Local mate competition
  • Nasonia
  • Parasitoid
  • Sex allocation
  • Transcriptomics

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